Monitoring SARS-CoV-2 Growth in Cell Culture

A Simplified Quantitative Real-Time PCR Assay for Monitoring SARS-CoV-2 Growth in Cell Culture

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected millions within just a few months, causing severe respiratory disease and mortality. Assays to monitor SARS-CoV-2 growth in vitro depend on time-consuming and costly RNA extraction steps, hampering progress in basic research and drug development efforts.
Here, we developed a simplified quantitative real-time PCR assay that bypasses viral RNA extraction steps and can monitor SARS-CoV-2 growth from a small amount of cell culture supernatants. In addition, we show that this approach is easily adaptable to numerous other RNA and DNA viruses. Using this assay, we screened the activities of a number of compounds that were predicted to alter SARS-CoV-2 entry and replication as well as HIV-1-specific drugs in a proof-of-concept study. We found that E64D (inhibitor of endosomal proteases cathepsin B and L) and apilimod (endosomal trafficking inhibitor) potently decreased the amount of SARS-CoV-2 RNA in cell culture supernatants with minimal cytotoxicity.
Surprisingly, we found that the macropinocytosis inhibitor ethylisopropylamiloride (EIPA) similarly decreased SARS-CoV-2 RNA levels in supernatants, suggesting that entry may additionally be mediated by an alternative pathway. HIV-1-specific inhibitors nevirapine (a nonnucleoside reverse transcriptase inhibitor [NNRTI]), amprenavir (a protease inhibitor), and allosteric integrase inhibitor 2 (ALLINI-2) modestly inhibited SARS-CoV-2 replication, albeit the 50% inhibitory concentration (IC50) values were much higher than that required for HIV-1. Taking the data together, this simplified assay will expedite basic SARS-CoV-2 research, be amenable to mid-throughput screening assays (i.e., drug, CRISPR, small interfering RNA [siRNA], etc.), and be applicable to a broad number of RNA and DNA viruses.
IMPORTANCE Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the etiological agent of the coronavirus disease 2019 (COVID-19) pandemic, is continuing to cause immense respiratory disease and social and economic disruptions. Conventional assays that monitor SARS-CoV-2 growth in cell culture rely on costly and time-consuming RNA extraction procedures, hampering progress in basic SARS-CoV-2 research and development of effective therapeutics.
Here, we developed a simple quantitative real-time PCR assay to monitor SARS-CoV-2 growth in cell culture supernatants that does not necessitate RNA extraction and that is as accurate and sensitive as existing methods. In a proof-of-concept screen, we found that E64D, apilimod, EIPA, and remdesivir can substantially impede SARS-Cov-2 replication, providing novel insight into viral entry and replication mechanisms.
In addition, we show that this approach is easily adaptable to numerous other RNA and DNA viruses. This simplified assay will undoubtedly expedite basic SARS-CoV-2 and virology research and be amenable to use in drug screening platforms to identify therapeutics against SARS-CoV-2.
pharmacodane

pharmacodane

rHu MIP-3-beta

AK8232-0005 5µg Ask for price

rHu MIP-3-beta

AK8232-0020 20µg Ask for price

rHu MIP-3-beta

AK8232-0100 100µg Ask for price

rHu MIP-3-beta

AK8232-1000 1mg Ask for price

rHu MIP-4

AK8218-0002 2µg Ask for price

rHu MIP-4

AK8218-0010 10µg Ask for price

rHu MIP-4

AK8218-0100 100µg Ask for price

rHu MIP-4

AK8218-1000 1mg Ask for price

rHu MIP-1-beta

AK8264-0002 2µg Ask for price

rHu MIP-1-beta

AK8264-0010 10µg Ask for price

rHu MIP-1-beta

AK8264-0100 100µg Ask for price

rHu MIP-1-beta

AK8264-1000 1mg Ask for price

MIP-3 ALPHA

GWB-70396B 0.02 mg Ask for price

MIP-3 Alpha Antibody

GWB-65BCF6 0.1 mg Ask for price

Monoclonal anti-human Alpha (FSH-Alpha), IgG clone 3 aff pure

FSHA12-M 1 mg
EUR 578.4

Rabbit Anti-Human Alpha Defensin 1-3 (NP1-3/hNP1-3)antiserum #1

HDEFA11-S 100 ul
EUR 548.4

Human Alpha Defensin 1-3 (NP1-3/hNP1-3)control/blocking peptide #1

HDEFA11-P 100 ug
EUR 196.8

MIP-3 alpha, CCL20, human

RC315-31 5ug
EUR 125.26

Human MIP-3 alpha (CCL20)

MBS691737-0005mg 0.005mg
EUR 300

Human MIP-3 alpha (CCL20)

MBS691737-002mg 0.02mg
EUR 450

Human MIP-3 alpha (CCL20)

MBS691737-5x002mg 5x0.02mg
EUR 1725

Mouse MIP-3 alpha (CCL20)

MBS692015-0005mg 0.005mg
EUR 300

Mouse MIP-3 alpha (CCL20)

MBS692015-002mg 0.02mg
EUR 450

Mouse MIP-3 alpha (CCL20)

MBS692015-5x002mg 5x0.02mg
EUR 1725

Anti-Human MIP-3&alpha

GWB-BIG752 500ug Ask for price

Ccl20 Antibody / Mip-3 alpha

R32858 100 ug
EUR 356.15
Description: Macrophage Inflammatory Protein 3 alpha (MIP-3 alpha), is also called Chemokine, cc motif, ligand 20 (CCL20). The MIP-3 alpha / CCL20 gene was cloned and sequenced, revealing a four exon, three intron structure, and was localized by FISH analysis to 2q35-q36. MIP3 alpha is predominantly expressed in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. MIP3 alpha / CCL20 and its receptor CCR6 are markedly up-regulated in psoriasis, and they may play a role in the recruitment of T cells to lesional psoriatic skin.

Rabbit Anti-Human Alpha Defensin 1-3 (NP1-3/hNP1-3), IgG #1, aff pure

HDEFA11-A 100 ug
EUR 578.4

Recombinant (E.Coli) Human p38 alpha/SAPK2 alpha

RP-675 1 ug
EUR 343.2

Murinoglobulin (alpha 1-inhibitor 3) Rat, purified protein

MGBN11-N-25 25 ug
EUR 270

MIP-3 alpha Recombinant Protein

40-353-0005mg 0.005 mg
EUR 311.1
Description: MIP-3 alpha is a CC chemokine that is expressed in the liver, lymph nodes, appendix, PBL and lung and signals through the CCR6 receptor. MIP-3 alpha is chemotactic towards lymphocytes and dendritic cells. Additionally, it promotes the adhesion of memory CD4+ T cells and inhibits colony formation of bone marrow myeloid immature progenitors. Recombinant murine MIP-3 alpha is a 7.9 kDa protein containing 70 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

MIP-3 alpha Recombinant Protein

40-353-002mg 0.02 mg
EUR 437.1
Description: MIP-3 alpha is a CC chemokine that is expressed in the liver, lymph nodes, appendix, PBL and lung and signals through the CCR6 receptor. MIP-3 alpha is chemotactic towards lymphocytes and dendritic cells. Additionally, it promotes the adhesion of memory CD4+ T cells and inhibits colony formation of bone marrow myeloid immature progenitors. Recombinant murine MIP-3 alpha is a 7.9 kDa protein containing 70 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

MIP-3 alpha Recombinant Protein

40-380-0005mg 0.005 mg
EUR 311.1
Description: MIP-3 alpha is a CC chemokine that is expressed in the liver, lymph nodes, appendix, PBL and lung and can signal through the CCR6 receptor. MIP-3 alpha is chemotactic towards lymphocytes and dendritic cells. Additionally, it promotes the adhesion of memory CD4+ T cells and inhibits colony formation of bone marrow myeloid immature progenitors. Recombinant human MIP-3 alpha is an 8.0 kDa protein containing 70 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

MIP-3 alpha Recombinant Protein

40-380-002mg 0.02 mg
EUR 437.1
Description: MIP-3 alpha is a CC chemokine that is expressed in the liver, lymph nodes, appendix, PBL and lung and can signal through the CCR6 receptor. MIP-3 alpha is chemotactic towards lymphocytes and dendritic cells. Additionally, it promotes the adhesion of memory CD4+ T cells and inhibits colony formation of bone marrow myeloid immature progenitors. Recombinant human MIP-3 alpha is an 8.0 kDa protein containing 70 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

MIP-3 alpha Polyclonal Antibody

BT-AP06995-100ul 100ul Ask for price
Description: This antimicrobial gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The protein encoded by this gene displays chemotactic activity for lymphocytes and can repress proliferation of myeloid progenitors. Two transcript variants encoding different isoforms have been found for this gene.

MIP-3 alpha Polyclonal Antibody

BT-AP06995-20ul 20ul Ask for price
Description: This antimicrobial gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The protein encoded by this gene displays chemotactic activity for lymphocytes and can repress proliferation of myeloid progenitors. Two transcript variants encoding different isoforms have been found for this gene.

MIP-3 alpha Polyclonal Antibody

BT-AP06995-50ul 50ul Ask for price
Description: This antimicrobial gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The protein encoded by this gene displays chemotactic activity for lymphocytes and can repress proliferation of myeloid progenitors. Two transcript variants encoding different isoforms have been found for this gene.

Recombinant (E.Coli) Human GRO-beta/MIP-2 (CXCL2)

RP-1013 10 ug
EUR 343.2

Recombinant Mouse (E.Coli) GRO-beta/MIP-2 (CXCL2)

RP-1040 5 ug
EUR 196.8

Recombinant (Sf90) bovine Phosphoinositide 3-kinase alpha p110a/p85a

RP-733 1 ug
EUR 343.2

Monoclonal anti-bovine GABAa Receptor alpha 1 (1-269aa) IgG #3, aff pure

GAA13-M 100 ug
EUR 578.4

human Topoisomerase II alpha (TOP2 alpha) Control/blocking peptide

TOP2A11-P 100 ug
EUR 196.8

MIP-1 ALPHA

GWB-2915BC 0.02 mg Ask for price

MIP-1 ALPHA

GWB-4CEC3A 0.02 mg Ask for price

MIP-1 ALPHA

GWB-C911A5 0.01 mg Ask for price

Recombinant purified, human Interleukin-1 alpha (IL-1 alpha), active

IL1A15-R-10 10 ug
EUR 416.4

Rabbit Anti-human Topoisomerase II alpha (TOP2 alpha) IgG, aff pure

TOP2A11-A 100 ug
EUR 578.4

Human/Rat GABAa Receptor alpha 3 (GAA3) Control/blocking peptide #1

GAA31-P 100 ug
EUR 196.8

RABBIT ANTI HUMAN MIP-3 ALPHA

MBS221726-01mg 0.1mg
EUR 635

RABBIT ANTI HUMAN MIP-3 ALPHA

MBS221726-5x01mg 5x0.1mg
EUR 2695

Mouse Anti-Human MIP-3 alpha

MBS690971-05mg 0.5mg
EUR 525

Mouse Anti-Human MIP-3 alpha

MBS690971-5x05mg 5x0.5mg
EUR 2080

Human Core-binding factor alpha 3 (CBFA3) Control/blocking peptide # 1

CBFA31-P 100 ug
EUR 196.8

Rabbit Anti-Human/Rat GABAa Receptor alpha 3 (GAA3) IgG, aff pure

GAA31-A 100 ug
EUR 578.4

Endothelial intergerin Alpha-V Beta-3 (Clone 17.16), RNA Aptamer, unlabeled

AR-287-U Custom Ask for price

Anti-Human MIP-3 alpha Antibody

101-M79 500 µg
EUR 246.75
Description: MIP-3α is a CC chemokine that is expressed in the liver, lymph nodes, appendix, PBL and lung and can signal through the CCR6 receptor. MIP-3α is chemotactic towards lymphocytes and dendritic cells. Additionally, it promotes the adhesion of memory CD4+ T cells and inhibits colony formation of bone marrow myeloid immature progenitors. Human MIP-3α is an 8.0 kDa protein containing 70 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

Anti-Human MIP-3 alpha Antibody

102-P67 100 µg
EUR 245.7
Description: MIP-3α is a CC chemokine that is expressed in the liver, lymph nodes, appendix, PBL and lung and can signal through the CCR6 receptor. MIP-3α is chemotactic towards lymphocytes and dendritic cells. Additionally, it promotes the adhesion of memory CD4+ T cells and inhibits colony formation of bone marrow myeloid immature progenitors. Recombinant human MIP-3α is an 8.0 kDa protein containing 70 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

Recombinant Human MIP-3 alpha (CCL20)

Z100705 25 µg
EUR 235
Description: Macrophage inflammatory protein (MIP)-3/CCL20, also known as liver and activation-regulated chemokine (LARC) or Exodus, is a member of the CC chemokine subfamily initially noted to be expressed in human liver, lung, appendix, and tonsillar crypts. MIP-3 is selectively chemotactic for CD34(+) bone marrow cell-derived immature DCs and CD45RO(+) memory T cells that express the cognate receptor CCR6. MIP-3 produced at sites of inflammation may chemoattract CCR6-expressing immature DCs to the subepithelial region of mucosal surfaces.

Recombinant Human MIP-3 alpha (CCL20)

Z100709 1.0 mg
EUR 3900
Description: Macrophage inflammatory protein (MIP)-3/CCL20, also known as liver and activation-regulated chemokine (LARC) or Exodus, is a member of the CC chemokine subfamily initially noted to be expressed in human liver, lung, appendix, and tonsillar crypts. MIP-3 is selectively chemotactic for CD34(+) bone marrow cell-derived immature DCs and CD45RO(+) memory T cells that express the cognate receptor CCR6. MIP-3 produced at sites of inflammation may chemoattract CCR6-expressing immature DCs to the subepithelial region of mucosal surfaces.

Recombinant Human MIP-3 alpha (CCL20)

HECCP-2001 5ug Ask for price

Recombinant Human MIP-3 alpha (CCL20)

MBS4159697-0025mg 0.025mg
EUR 385

Recombinant Human MIP-3 alpha (CCL20)

MBS4159697-1mg 1(mg)
EUR 4600

Recombinant Human MIP-3 alpha (CCL20)

MBS4159697-5x1mg 5x1mg
EUR 20445

Human MIP-3 alpha (CCL20) Recombinant

MBS553195-0005mg 0.005mg
EUR 165

Human MIP-3 alpha (CCL20) Recombinant

MBS553195-0025mg 0.025mg
EUR 245

Recombinant Human MIP-3 alpha/CCL20

P1220-.005 5ug
EUR 108.8
Description: Peptides & Proteins|Recombinant Proteins

Recombinant Human MIP-3 alpha/CCL20

P1220-.1 100ug
EUR 900.8
Description: Peptides & Proteins|Recombinant Proteins

Recombinant Human MIP-3 alpha/CCL20

P1220-.5 500ug
EUR 2020.8
Description: Peptides & Proteins|Recombinant Proteins

Murinoglobulin (alpha 1-inhibitor 3) Rat, purified protein control for western

MGBN11-C 100 ul
EUR 343.2

Rabbit Anti-Rat Murinoglobulin (alpha 1-inhibitor 3) Rat, IgG, aff pure

MGBN11-A 100 ug
EUR 578.4

Rabbit polyclonal MIP-3 alpha antibody

TA319360 100 µg Ask for price

Rabbit Anti-human Core-binding factor alpha 3 (CBFA3) aff pure IgG # 1

CBFA31-A 100 ul
EUR 578.4

Rabbit polyclonal MIP 3 alpha antibody

TA319370 100 µg Ask for price

Recombinant Human Alpha-Synuclein

RP-847 20 ug
EUR 196.8

Monoclonal Anti-Human Hypoxia-inducible factor-1 alpha (HIF1A) IgG #3, aff pure

HIF1A13-M 100 ug
EUR 578.4

Alpha 2-Antiplasmin, Human Plasma

A2AP15-100 100 ug
EUR 196.8

Alpha 1-Antitrypsin, Human Plasma

A1AT15-N 1 mg
EUR 196.8

Rabbit Anti-Rat Glutathione Transferase alpha (GST-alpha) antiserum

GSTA11-S 100 ul
EUR 548.4

Rabbit Anti-Human Glutathione Transferase alpha (GST-alpha) antiserum

GSTA12-S 100 ul
EUR 548.4

Rabbit Anti-Human Estrogen receptor alpha (ER-alpha) peptide IgG

ERA12-A 100 ug
EUR 578.4

Rat MIP-3 Alpha/CCL20 ELISA Kit

EK5193 96 tests
EUR 599

Rat MIP-3 alpha/CCL20 ELISA Kit

GWB-4GLO39 1x96 well plate Ask for price

Mouse alpha Macroglobulin protein, pure

AMGL15-N-25 25 ug
EUR 270

Human hypoxia-inducible transcription factor 1 alpha (HIF-1 alpha) ELISA Kit, 96 tests, Quantitative

100-530-HIF 1 Kit
EUR 854.4

Alpha 1-Antichymotrypsin , Human Plasma

A1CT15-N-100 100 ug
EUR 196.8

Goat Anti-Human Lutenizing Hormone Alpha (LH-Alpha) IgG aff pure

LHA13-A 1 mg
EUR 343.2

Expression of ADR-α1, 2 and ADR-β2 in cumulus cellculture of infertile women with polycystic ovary syndrome and poor responder who are a candidate for IVF: the novel strategic role of clonidine in this expression

Objective: Alpha and beta-adrenoceptors (ADR-α1, 2, and β2) play a regulatory role in the folliculogenesis and steroidogenesis in the ovarian follicles. This study aimed to measure these adrenoceptors mRNA and its protein levels in cumulus cells (CCs) culture of poor ovarian reserve (POR) and polycystic ovarian syndrome (PCOS) infertile women (IVF candidate) and the effect of clonidine treatment at CCs culture.
Methods: This case/control study was conducted in 2017 includes a control (donation oocytes) and two studies (PCO and POR) groups. The ovulation induction drugs were prescribed in all groups. After the oocyte puncture, the follicular fluid was collected and CCs were isolated were cultured. RNA was extracted and cDNA was synthesized and designed the primer for the ADR-α1, 2 and ADR-β2 gene expression. The protein levels were investigated by Western Blot.
Results: The results showed a high level of three adrenergic expressions in PCO women compared to the control group (p-value <.001), which can be reduced by clonidine. POR group showed a significant decrease in the gene expression of ADR-α1 (p-value = .004) and ADR-α2 (p-value = .003) compared to the control group and clonidine treatment had no effect.
Conclusion: The significant increase of three adrenoceptors gene expression and protein levels in CCs culture indicate to the hyperactivity of the ovarian sympathetic nervous system at the receptor levels in women with PCOS, and clonidine confirmed it by reducing this expression. In POR women, the reduction of ADR-α1, 2 expressions maybe lead to the aging process in the ovary.

VEGF 121, CF

PR15029CF 5 ug
EUR 498

VEGF-121-Fc

E13-m005-1 10μg
EUR 225
Description: N/A

VEGF-121-Fc

E13-m005-2 50μg
EUR 1125
Description: N/A

VEGF-121 (His tag)

EGPS002 10ug
EUR 175

Human VEGF 121 Antibody

32562-05111 150 ug
EUR 215

VEGF 121 Recombinant Protein

96-768 0.02 mg
EUR 519
Description: Vascular endothelial growth factor (VEGF) is also known as vascular permeability factor (VPF) and VEGF-A, and is a member of the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. Alternatively spliced isoforms of 121,145,165,183,189 and 206 amino acids in length are expressed in humans. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189. VEGF121 is the only form that lacks a basic heparinbinding region and is freely diffusible. Mouse embryos expressing only the corresponding isoform (VEGF120) do not survive to term, and show defects in skeletogenesis. Human VEGF121 shares 87% aa sequence identity with corresponding regions of mouse and rat, 93% with feline, equine and bovine, and 91%, 95% and 96% with ovine, canine and porcine VEGF, respectively. VEGF121 induces the proliferation of lymphatic endothelial cells. The lymphangiogenesis may be promoted by upregulation of VEGF121, which may in turn act in part via induction of VEGF-C.

rHu EG-VEGF

AK8315-0005 5µg Ask for price

rHu EG-VEGF

AK8315-0020 20µg Ask for price

rHu EG-VEGF

AK8315-0100 100µg Ask for price

rHu EG-VEGF

AK8315-1000 1mg Ask for price

Recombinant Human VEGF 121 Protein

E40KMP1027 20ug
EUR 495

Recombinant human VEGF 121 Protein

MBS9144069-0005mg 0.005mg
EUR 145

Recombinant human VEGF 121 Protein

MBS9144069-002mg 0.02mg
EUR 195

Recombinant human VEGF 121 Protein

MBS9144069-005mg 0.05mg
EUR 255

Recombinant human VEGF 121 Protein

MBS9144069-01mg 0.1mg
EUR 335

Recombinant human VEGF 121 Protein

MBS9144069-5x01mg 5x0.1mg
EUR 1330

OPSD00063-2UG - Recombinant VEGF-121

OPSD00063-2UG 2ug
EUR 108

Recombinant Human VEGF165 (rHu VEGF165)

70505 10ul
EUR 235
Description: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human umbilical vein endothelial cells(HUVEC) is between 1.0-8.0 ng/ml.

Recombinant Human VEGF165 (rHu VEGF165)

MBS9419932-001mL 0.01mL
EUR 280

Recombinant Human VEGF165 (rHu VEGF165)

MBS9419932-5x001mL 5x0.01mL
EUR 1120

Vascular Endothelial Growth Factor-121, Recombinant, Human (VEGF-121)

MBS650596-0002mg 0.002mg
EUR 340

Vascular Endothelial Growth Factor-121, Recombinant, Human (VEGF-121)

MBS650596-001mg 0.01mg
EUR 490

Vascular Endothelial Growth Factor-121, Recombinant, Human (VEGF-121)

MBS650596-5x001mg 5x0.01mg
EUR 2055

Vascular Endothelial Growth Factor-121, Recombinant, Human (VEGF-121)

MBS650732-0002mg 0.002mg
EUR 375

Vascular Endothelial Growth Factor-121, Recombinant, Human (VEGF-121)

MBS650732-001mg 0.01mg
EUR 455

Vascular Endothelial Growth Factor-121, Recombinant, Human (VEGF-121)

MBS650732-5x001mg 5x0.01mg
EUR 1890

Human CellExp? VEGF 121, Human Recombinant

6484-10 each
EUR 411.6

Human CellExp? VEGF 121, Human Recombinant

6484-50 each
EUR 1599.6

Human VEGF 121 Antibody (Biotin Conjugate)

32562-05121 150 ug
EUR 315

Recombinant human VEGF 121 Protein, His Tag

E40KMP1359 20ug
EUR 495

VEGF 121 Recombinant Protein Tag free Lyophilized

IVEGF121RLY20UG each
EUR 592
Description: VEGF 121 Recombinant Protein Tag free Lyophilized

VEGF 121 Recombinant Protein Tag free Lyophilized

MBS8434669-INQUIRE INQUIRE Ask for price

Vascular Endothelial Growth Factor-121, aa207-327, Recombinant, Human (VEGF-121)

MBS636283-001mg 0.01mg
EUR 750

Vascular Endothelial Growth Factor-121, aa207-327, Recombinant, Human (VEGF-121)

MBS636283-5x001mg 5x0.01mg
EUR 3235

Vascular Endothelial Growth Factor-121, Recombinant, Human, aa207-327 (VEGF-121)

MBS650154-005mg 0.05mg
EUR 610

Vascular Endothelial Growth Factor-121, Recombinant, Human, aa207-327 (VEGF-121)

MBS650154-5x005mg 5x0.05mg
EUR 2600

Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF) (AP)

MBS6505503-01mL 0.1mL
EUR 1035

Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF) (AP)

MBS6505503-5x01mL 5x0.1mL
EUR 4515

Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF) (PE)

MBS6505512-01mL 0.1mL
EUR 1035

Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF) (PE)

MBS6505512-5x01mL 5x0.1mL
EUR 4515

Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF) (AP)

MBS6505513-01mL 0.1mL
EUR 1115

Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF) (AP)

MBS6505513-5x01mL 5x0.1mL
EUR 4875

Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF) (PE)

MBS6505523-01mL 0.1mL
EUR 1115

Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF) (PE)

MBS6505523-5x01mL 5x0.1mL
EUR 4875

Vascular Endothelial Growth Factor, Human (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF)

MBS634869-005mg 0.05mg
EUR 415

Vascular Endothelial Growth Factor, Human (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF)

MBS634869-5x005mg 5x0.05mg
EUR 1725

Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF) (APC)

MBS6505504-01mL 0.1mL
EUR 1035

Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF) (APC)

MBS6505504-5x01mL 5x0.1mL
EUR 4515

Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF) (FITC)

MBS6505505-01mL 0.1mL
EUR 1035

Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF) (FITC)

MBS6505505-5x01mL 5x0.1mL
EUR 4515

Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF) (HRP)

MBS6505506-01mL 0.1mL
EUR 1035

Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF) (HRP)

MBS6505506-5x01mL 5x0.1mL
EUR 4515

Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF) (APC)

MBS6505514-01mL 0.1mL
EUR 1115

Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF) (APC)

MBS6505514-5x01mL 5x0.1mL
EUR 4875

Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF) (Biotin)

MBS6505515-01mL 0.1mL
EUR 1115

Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF) (Biotin)

MBS6505515-5x01mL 5x0.1mL
EUR 4875

Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF) (FITC)

MBS6505516-01mL 0.1mL
EUR 1115

Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF) (FITC)

MBS6505516-5x01mL 5x0.1mL
EUR 4875

Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF) (HRP)

MBS6505517-01mL 0.1mL
EUR 1115

Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF) (HRP)

MBS6505517-5x01mL 5x0.1mL
EUR 4875

Recombinant Human VEGF121 Protein

PROTP15692-10 10ug
EUR 380.4
Description: VEGF is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, VEGF plays a prominent role in normal and pathological angiogenesis. VEGF signals through three receptors; fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product. Due to its increased acidity, VEGF121 circulates more freely than other VEGF forms, which bind more tightly with vascular heparin sulfates. Recombinant human VEGF121 is a 28.4 kDa disulfide-linked homodimeric protein consisting of two 121 amino acid polypeptide chains.

Human VEGF 121 AssayLite™ Antibody (RPE Conjugate)

32562-05151 150 ug
EUR 370

Human VEGF 121 AssayLite™ Antibody (APC Conjugate)

32562-05161 150 ug
EUR 370

Human VEGF 121 AssayLite™ Antibody (FITC Conjugate)

32562-05141 150 ug
EUR 370

Human VEGF 121 AssayLite™ Antibody (PerCP Conjugate)

32562-05171 150 ug
EUR 410

Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF) (MaxLight 405)

MBS6505507-01mL 0.1mL
EUR 1035

Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF) (MaxLight 405)

MBS6505507-5x01mL 5x0.1mL
EUR 4515

Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF) (MaxLight 490)

MBS6505508-01mL 0.1mL
EUR 1035

Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF) (MaxLight 490)

MBS6505508-5x01mL 5x0.1mL
EUR 4515

Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF) (MaxLight 550)

MBS6505509-01mL 0.1mL
EUR 1035

Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF) (MaxLight 550)

MBS6505509-5x01mL 5x0.1mL
EUR 4515

Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF) (MaxLight 650)

MBS6505510-01mL 0.1mL
EUR 1035

Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF) (MaxLight 650)

MBS6505510-5x01mL 5x0.1mL
EUR 4515

Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF) (MaxLight 750)

MBS6505511-01mL 0.1mL
EUR 1035

Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF) (MaxLight 750)

MBS6505511-5x01mL 5x0.1mL
EUR 4515

Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF) (MaxLight 405)

MBS6505518-01mL 0.1mL
EUR 1115

Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF) (MaxLight 405)

MBS6505518-5x01mL 5x0.1mL
EUR 4875

Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF) (MaxLight 490)

MBS6505519-01mL 0.1mL
EUR 1115

Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF) (MaxLight 490)

MBS6505519-5x01mL 5x0.1mL
EUR 4875

Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF) (MaxLight 550)

MBS6505520-01mL 0.1mL
EUR 1115

Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF) (MaxLight 550)

MBS6505520-5x01mL 5x0.1mL
EUR 4875

Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF) (MaxLight 650)

MBS6505521-01mL 0.1mL
EUR 1115

Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF) (MaxLight 650)

MBS6505521-5x01mL 5x0.1mL
EUR 4875

Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF) (MaxLight 750)

MBS6505522-01mL 0.1mL
EUR 1115

Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF) (MaxLight 750)

MBS6505522-5x01mL 5x0.1mL
EUR 4875

VEGF (121 a.a.), Vascular Endothelial Growth Factor-121 Human Recombinant Protein, His Tag

PROTP15692-8 Regular: 10ug
EUR 380.4
Description: Vascular Endothelial Growth Factor-121 Human Recombinant produced in E.Coli is a double, non-glycosylated, polypeptide chain (aa 207-327) containing a total of 142 amino acids and having a molecular mass of 16.3 kDa. ;The VEGF-121 is fused to a 20 amino acid His tag at N-terminus and purified by proprietary chromatographic techniques.

Vascular Endothelial Growth Factor-121, aa207-327, Recombinant, Human (VEGF-121) (BSA Free)

MBS635981-001mg 0.01mg
EUR 750