Monitoring SARS-CoV-2 Growth in Cell Culture

A Simplified Quantitative Real-Time PCR Assay for Monitoring SARS-CoV-2 Growth in Cell Culture

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected millions within just a few months, causing severe respiratory disease and mortality. Assays to monitor SARS-CoV-2 growth in vitro depend on time-consuming and costly RNA extraction steps, hampering progress in basic research and drug development efforts.
Here, we developed a simplified quantitative real-time PCR assay that bypasses viral RNA extraction steps and can monitor SARS-CoV-2 growth from a small amount of cell culture supernatants. In addition, we show that this approach is easily adaptable to numerous other RNA and DNA viruses. Using this assay, we screened the activities of a number of compounds that were predicted to alter SARS-CoV-2 entry and replication as well as HIV-1-specific drugs in a proof-of-concept study. We found that E64D (inhibitor of endosomal proteases cathepsin B and L) and apilimod (endosomal trafficking inhibitor) potently decreased the amount of SARS-CoV-2 RNA in cell culture supernatants with minimal cytotoxicity.
Surprisingly, we found that the macropinocytosis inhibitor ethylisopropylamiloride (EIPA) similarly decreased SARS-CoV-2 RNA levels in supernatants, suggesting that entry may additionally be mediated by an alternative pathway. HIV-1-specific inhibitors nevirapine (a nonnucleoside reverse transcriptase inhibitor [NNRTI]), amprenavir (a protease inhibitor), and allosteric integrase inhibitor 2 (ALLINI-2) modestly inhibited SARS-CoV-2 replication, albeit the 50% inhibitory concentration (IC50) values were much higher than that required for HIV-1. Taking the data together, this simplified assay will expedite basic SARS-CoV-2 research, be amenable to mid-throughput screening assays (i.e., drug, CRISPR, small interfering RNA [siRNA], etc.), and be applicable to a broad number of RNA and DNA viruses.
IMPORTANCE Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the etiological agent of the coronavirus disease 2019 (COVID-19) pandemic, is continuing to cause immense respiratory disease and social and economic disruptions. Conventional assays that monitor SARS-CoV-2 growth in cell culture rely on costly and time-consuming RNA extraction procedures, hampering progress in basic SARS-CoV-2 research and development of effective therapeutics.
Here, we developed a simple quantitative real-time PCR assay to monitor SARS-CoV-2 growth in cell culture supernatants that does not necessitate RNA extraction and that is as accurate and sensitive as existing methods. In a proof-of-concept screen, we found that E64D, apilimod, EIPA, and remdesivir can substantially impede SARS-Cov-2 replication, providing novel insight into viral entry and replication mechanisms.
In addition, we show that this approach is easily adaptable to numerous other RNA and DNA viruses. This simplified assay will undoubtedly expedite basic SARS-CoV-2 and virology research and be amenable to use in drug screening platforms to identify therapeutics against SARS-CoV-2.
pharmacodane

pharmacodane

rHu MIP-3-beta

AK8232-0005 5µg Ask for price

rHu MIP-3-beta

AK8232-0020 20µg Ask for price

rHu MIP-3-beta

AK8232-0100 100µg Ask for price

rHu MIP-3-beta

AK8232-1000 1mg Ask for price

rHu MIP-4

AK8218-0002 2µg Ask for price

rHu MIP-4

AK8218-0010 10µg Ask for price

rHu MIP-4

AK8218-0100 100µg Ask for price

rHu MIP-4

AK8218-1000 1mg Ask for price

rHu MIP-1-beta

AK8264-0002 2µg Ask for price

rHu MIP-1-beta

AK8264-0010 10µg Ask for price

rHu MIP-1-beta

AK8264-0100 100µg Ask for price

rHu MIP-1-beta

AK8264-1000 1mg Ask for price

MIP-3 ALPHA

GWB-70396B 0.02 mg Ask for price

MIP-3 Alpha Antibody

GWB-65BCF6 0.1 mg Ask for price

MIP-3 alpha, CCL20, human

RC315-31 5ug
EUR 125.26

Anti-Human MIP-3&alpha

GWB-BIG752 500ug Ask for price

Ccl20 Antibody / Mip-3 alpha

R32858 100 ug
EUR 356.15
Description: Macrophage Inflammatory Protein 3 alpha (MIP-3 alpha), is also called Chemokine, cc motif, ligand 20 (CCL20). The MIP-3 alpha / CCL20 gene was cloned and sequenced, revealing a four exon, three intron structure, and was localized by FISH analysis to 2q35-q36. MIP3 alpha is predominantly expressed in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. MIP3 alpha / CCL20 and its receptor CCR6 are markedly up-regulated in psoriasis, and they may play a role in the recruitment of T cells to lesional psoriatic skin.

Monoclonal anti-human Alpha (FSH-Alpha), IgG clone 3 aff pure

FSHA12-M 1 mg
EUR 578.4

MIP-3 alpha Recombinant Protein

40-353-0005mg 0.005 mg
EUR 311.1
Description: MIP-3 alpha is a CC chemokine that is expressed in the liver, lymph nodes, appendix, PBL and lung and signals through the CCR6 receptor. MIP-3 alpha is chemotactic towards lymphocytes and dendritic cells. Additionally, it promotes the adhesion of memory CD4+ T cells and inhibits colony formation of bone marrow myeloid immature progenitors. Recombinant murine MIP-3 alpha is a 7.9 kDa protein containing 70 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

MIP-3 alpha Recombinant Protein

40-353-002mg 0.02 mg
EUR 437.1
Description: MIP-3 alpha is a CC chemokine that is expressed in the liver, lymph nodes, appendix, PBL and lung and signals through the CCR6 receptor. MIP-3 alpha is chemotactic towards lymphocytes and dendritic cells. Additionally, it promotes the adhesion of memory CD4+ T cells and inhibits colony formation of bone marrow myeloid immature progenitors. Recombinant murine MIP-3 alpha is a 7.9 kDa protein containing 70 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

MIP-3 alpha Recombinant Protein

40-380-0005mg 0.005 mg
EUR 311.1
Description: MIP-3 alpha is a CC chemokine that is expressed in the liver, lymph nodes, appendix, PBL and lung and can signal through the CCR6 receptor. MIP-3 alpha is chemotactic towards lymphocytes and dendritic cells. Additionally, it promotes the adhesion of memory CD4+ T cells and inhibits colony formation of bone marrow myeloid immature progenitors. Recombinant human MIP-3 alpha is an 8.0 kDa protein containing 70 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

MIP-3 alpha Recombinant Protein

40-380-002mg 0.02 mg
EUR 437.1
Description: MIP-3 alpha is a CC chemokine that is expressed in the liver, lymph nodes, appendix, PBL and lung and can signal through the CCR6 receptor. MIP-3 alpha is chemotactic towards lymphocytes and dendritic cells. Additionally, it promotes the adhesion of memory CD4+ T cells and inhibits colony formation of bone marrow myeloid immature progenitors. Recombinant human MIP-3 alpha is an 8.0 kDa protein containing 70 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

Recombinant (E.Coli) Human p38 alpha/SAPK2 alpha

RP-675 1 ug
EUR 343.2

MIP-1 ALPHA

GWB-2915BC 0.02 mg Ask for price

MIP-1 ALPHA

GWB-4CEC3A 0.02 mg Ask for price

MIP-1 ALPHA

GWB-C911A5 0.01 mg Ask for price

Rabbit Anti-Human Alpha Defensin 1-3 (NP1-3/hNP1-3)antiserum #1

HDEFA11-S 100 ul
EUR 548.4

Human Alpha Defensin 1-3 (NP1-3/hNP1-3)control/blocking peptide #1

HDEFA11-P 100 ug
EUR 196.8

Murinoglobulin (alpha 1-inhibitor 3) Rat, purified protein

MGBN11-N-25 25 ug
EUR 270

human Topoisomerase II alpha (TOP2 alpha) Control/blocking peptide

TOP2A11-P 100 ug
EUR 196.8

Rabbit Anti-Human Alpha Defensin 1-3 (NP1-3/hNP1-3), IgG #1, aff pure

HDEFA11-A 100 ug
EUR 578.4

Recombinant purified, human Interleukin-1 alpha (IL-1 alpha), active

IL1A15-R-10 10 ug
EUR 416.4

Anti-Human MIP-3 alpha Antibody

101-M79 500 µg
EUR 246.75
Description: MIP-3α is a CC chemokine that is expressed in the liver, lymph nodes, appendix, PBL and lung and can signal through the CCR6 receptor. MIP-3α is chemotactic towards lymphocytes and dendritic cells. Additionally, it promotes the adhesion of memory CD4+ T cells and inhibits colony formation of bone marrow myeloid immature progenitors. Human MIP-3α is an 8.0 kDa protein containing 70 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

Anti-Human MIP-3 alpha Antibody

102-P67 100 µg
EUR 245.7
Description: MIP-3α is a CC chemokine that is expressed in the liver, lymph nodes, appendix, PBL and lung and can signal through the CCR6 receptor. MIP-3α is chemotactic towards lymphocytes and dendritic cells. Additionally, it promotes the adhesion of memory CD4+ T cells and inhibits colony formation of bone marrow myeloid immature progenitors. Recombinant human MIP-3α is an 8.0 kDa protein containing 70 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

Rabbit Anti-human Topoisomerase II alpha (TOP2 alpha) IgG, aff pure

TOP2A11-A 100 ug
EUR 578.4

Recombinant Human MIP-3 alpha (CCL20)

HECCP-2001 5ug Ask for price

Recombinant Human MIP-3 alpha (CCL20)

Z100705 25 µg
EUR 235
Description: Macrophage inflammatory protein (MIP)-3/CCL20, also known as liver and activation-regulated chemokine (LARC) or Exodus, is a member of the CC chemokine subfamily initially noted to be expressed in human liver, lung, appendix, and tonsillar crypts. MIP-3 is selectively chemotactic for CD34(+) bone marrow cell-derived immature DCs and CD45RO(+) memory T cells that express the cognate receptor CCR6. MIP-3 produced at sites of inflammation may chemoattract CCR6-expressing immature DCs to the subepithelial region of mucosal surfaces.

Recombinant Human MIP-3 alpha (CCL20)

Z100709 1.0 mg
EUR 3900
Description: Macrophage inflammatory protein (MIP)-3/CCL20, also known as liver and activation-regulated chemokine (LARC) or Exodus, is a member of the CC chemokine subfamily initially noted to be expressed in human liver, lung, appendix, and tonsillar crypts. MIP-3 is selectively chemotactic for CD34(+) bone marrow cell-derived immature DCs and CD45RO(+) memory T cells that express the cognate receptor CCR6. MIP-3 produced at sites of inflammation may chemoattract CCR6-expressing immature DCs to the subepithelial region of mucosal surfaces.

Monoclonal anti-bovine GABAa Receptor alpha 1 (1-269aa) IgG #3, aff pure

GAA13-M 100 ug
EUR 578.4

Recombinant (E.Coli) Human GRO-beta/MIP-2 (CXCL2)

RP-1013 10 ug
EUR 343.2

Recombinant Mouse (E.Coli) GRO-beta/MIP-2 (CXCL2)

RP-1040 5 ug
EUR 196.8

Recombinant (Sf90) bovine Phosphoinositide 3-kinase alpha p110a/p85a

RP-733 1 ug
EUR 343.2

Rabbit polyclonal MIP-3 alpha antibody

TA319360 100 µg Ask for price

Rabbit polyclonal MIP 3 alpha antibody

TA319370 100 µg Ask for price

MIP-1 alpha Antibody

20-abx148975
  • Ask for price
  • Ask for price
  • 100 ug
  • 50 ug

MIP-1 alpha Antibody

45363-100ul 100ul
EUR 302.4

MIP-1 alpha Antibody

45363-50ul 50ul
EUR 224.4

MIP-1 alpha Antibody

E19-8572-1 50ug/50ul
EUR 145
Description: Available in various conjugation types.

MIP-1 alpha Antibody

E19-8572-2 100ug/100ul
EUR 225
Description: Available in various conjugation types.

MIP-1 alpha Antibody

DF8572 200ul
EUR 420

MIP-1 alpha Antibody

DF8572-100ul 100ul
EUR 280

MIP-1 alpha Antibody

DF8572-200ul 200ul
EUR 350

MIP-1 Alpha Antibody

GWB-909B51 0.1 mg Ask for price

Human/Rat GABAa Receptor alpha 3 (GAA3) Control/blocking peptide #1

GAA31-P 100 ug
EUR 196.8

MIP 1 alpha antibody

20R-1788 100 ug
EUR 807.6
Description: Rabbit polyclonal MIP 1 alpha antibody

MIP- 1 alpha Antibody

ABD8572 100 ug
EUR 525.6

Human Core-binding factor alpha 3 (CBFA3) Control/blocking peptide # 1

CBFA31-P 100 ug
EUR 196.8

Rabbit Anti-Human/Rat GABAa Receptor alpha 3 (GAA3) IgG, aff pure

GAA31-A 100 ug
EUR 578.4

Rat MIP-3 Alpha/CCL20 ELISA Kit

EK5193 96 tests
EUR 599

Rat MIP-3 alpha/CCL20 ELISA Kit

GWB-4GLO39 1x96 well plate Ask for price

Recombinant Human Alpha-Synuclein

RP-847 20 ug
EUR 196.8

Endothelial intergerin Alpha-V Beta-3 (Clone 17.16), RNA Aptamer, unlabeled

AR-287-U Custom Ask for price

Human MIP-3 Alpha/CCL20 ELISA Kit

EK5191 96 tests
EUR 599

Mouse MIP-3 Alpha/CCL20 ELISA Kit

EK5192 96 tests
EUR 599

Human MIP-3 alpha/CCL20 ELISA Kit

GWB-R8MPA6 1x96 well plate Ask for price

Mouse MIP-3 alpha/CCL20 ELISA Kit

LF-EK50839 1×96T
EUR 777.6

Mouse MIP-3 alpha/CCL20 ELISA Kit

RK00129 96T
EUR 280

Human MIP-3 alpha/CCL20 ELISA Kit

RK00207 96 Tests
EUR 280

Alpha 2-Antiplasmin, Human Plasma

A2AP15-100 100 ug
EUR 196.8

Alpha 1-Antitrypsin, Human Plasma

A1AT15-N 1 mg
EUR 196.8

Rabbit Anti-Rat Murinoglobulin (alpha 1-inhibitor 3) Rat, IgG, aff pure

MGBN11-A 100 ug
EUR 578.4

Murinoglobulin (alpha 1-inhibitor 3) Rat, purified protein control for western

MGBN11-C 100 ul
EUR 343.2

Rabbit Anti-Rat Glutathione Transferase alpha (GST-alpha) antiserum

GSTA11-S 100 ul
EUR 548.4

Mouse alpha Macroglobulin protein, pure

AMGL15-N-25 25 ug
EUR 270

Rabbit Anti-human Core-binding factor alpha 3 (CBFA3) aff pure IgG # 1

CBFA31-A 100 ul
EUR 578.4

Rabbit Anti-Human Estrogen receptor alpha (ER-alpha) peptide IgG

ERA12-A 100 ug
EUR 578.4

Rabbit Anti-Human Glutathione Transferase alpha (GST-alpha) antiserum

GSTA12-S 100 ul
EUR 548.4

Alpha 1-Antichymotrypsin , Human Plasma

A1CT15-N-100 100 ug
EUR 196.8

Human hypoxia-inducible transcription factor 1 alpha (HIF-1 alpha) ELISA Kit, 96 tests, Quantitative

100-530-HIF 1 Kit
EUR 854.4

Goat Anti-Human Lutenizing Hormone Alpha (LH-Alpha) IgG aff pure

LHA13-A 1 mg
EUR 343.2

Recombinant (E.Coli) Human Thyrostimulin Alpha

RP-595 10 ug
EUR 343.2

Recombinant (E.Coli) Human Heat Shock Protein 90 alpha (hsp90/hsp90 alpha/dnak) protein control for Western

HSP903-C 100ul
EUR 343.2

Goat Anti-Ferret IgA (alpha), unlabeled

70519 0.5 mg
EUR 255.6

Alpha 2-HS Glycoprotein, Human Plasma

A2HG15-N 1 mg
EUR 343.2

Rabbit Anti-Human alpha-Tubulin IgG

TUBA13-A 100 ul
EUR 578.4

Expression of ADR-α1, 2 and ADR-β2 in cumulus cellculture of infertile women with polycystic ovary syndrome and poor responder who are a candidate for IVF: the novel strategic role of clonidine in this expression

Objective: Alpha and beta-adrenoceptors (ADR-α1, 2, and β2) play a regulatory role in the folliculogenesis and steroidogenesis in the ovarian follicles. This study aimed to measure these adrenoceptors mRNA and its protein levels in cumulus cells (CCs) culture of poor ovarian reserve (POR) and polycystic ovarian syndrome (PCOS) infertile women (IVF candidate) and the effect of clonidine treatment at CCs culture.
Methods: This case/control study was conducted in 2017 includes a control (donation oocytes) and two studies (PCO and POR) groups. The ovulation induction drugs were prescribed in all groups. After the oocyte puncture, the follicular fluid was collected and CCs were isolated were cultured. RNA was extracted and cDNA was synthesized and designed the primer for the ADR-α1, 2 and ADR-β2 gene expression. The protein levels were investigated by Western Blot.
Results: The results showed a high level of three adrenergic expressions in PCO women compared to the control group (p-value <.001), which can be reduced by clonidine. POR group showed a significant decrease in the gene expression of ADR-α1 (p-value = .004) and ADR-α2 (p-value = .003) compared to the control group and clonidine treatment had no effect.
Conclusion: The significant increase of three adrenoceptors gene expression and protein levels in CCs culture indicate to the hyperactivity of the ovarian sympathetic nervous system at the receptor levels in women with PCOS, and clonidine confirmed it by reducing this expression. In POR women, the reduction of ADR-α1, 2 expressions maybe lead to the aging process in the ovary.

VEGF 121, CF

PR15029CF 5 ug
EUR 498

VEGF-121-Fc

E13-m005-1 10μg
EUR 225
Description: N/A

VEGF-121-Fc

E13-m005-2 50μg
EUR 1125
Description: N/A

VEGF-121 (His tag)

EGPS002 10ug
EUR 175

Human VEGF 121 Antibody

32562-05111 150 ug
EUR 215

VEGF 121 Recombinant Protein

96-768 0.02 mg
EUR 519
Description: Vascular endothelial growth factor (VEGF) is also known as vascular permeability factor (VPF) and VEGF-A, and is a member of the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. Alternatively spliced isoforms of 121,145,165,183,189 and 206 amino acids in length are expressed in humans. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189. VEGF121 is the only form that lacks a basic heparinbinding region and is freely diffusible. Mouse embryos expressing only the corresponding isoform (VEGF120) do not survive to term, and show defects in skeletogenesis. Human VEGF121 shares 87% aa sequence identity with corresponding regions of mouse and rat, 93% with feline, equine and bovine, and 91%, 95% and 96% with ovine, canine and porcine VEGF, respectively. VEGF121 induces the proliferation of lymphatic endothelial cells. The lymphangiogenesis may be promoted by upregulation of VEGF121, which may in turn act in part via induction of VEGF-C.

rHu EG-VEGF

AK8315-0005 5µg Ask for price

rHu EG-VEGF

AK8315-0020 20µg Ask for price

rHu EG-VEGF

AK8315-0100 100µg Ask for price

rHu EG-VEGF

AK8315-1000 1mg Ask for price

Recombinant Human VEGF 121 Protein

E40KMP1027 20ug
EUR 495

OPSD00063-2UG - Recombinant VEGF-121

OPSD00063-2UG 2ug
EUR 108

Human CellExp? VEGF 121, Human Recombinant

6484-10 each
EUR 411.6

Human CellExp? VEGF 121, Human Recombinant

6484-50 each
EUR 1599.6

Human VEGF 121 Antibody (Biotin Conjugate)

32562-05121 150 ug
EUR 315

Recombinant Human VEGF165 (rHu VEGF165)

70505 10ul
EUR 235
Description: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human umbilical vein endothelial cells(HUVEC) is between 1.0-8.0 ng/ml.

Recombinant human VEGF 121 Protein, His Tag

E40KMP1359 20ug
EUR 495

VEGF 121 Recombinant Protein Tag free Lyophilized

IVEGF121RLY20UG each
EUR 592
Description: VEGF 121 Recombinant Protein Tag free Lyophilized

Recombinant Human VEGF121 Protein

PROTP15692-10 10ug
EUR 380.4
Description: VEGF is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, VEGF plays a prominent role in normal and pathological angiogenesis. VEGF signals through three receptors; fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product. Due to its increased acidity, VEGF121 circulates more freely than other VEGF forms, which bind more tightly with vascular heparin sulfates. Recombinant human VEGF121 is a 28.4 kDa disulfide-linked homodimeric protein consisting of two 121 amino acid polypeptide chains.

Human VEGF 121 AssayLite™ Antibody (RPE Conjugate)

32562-05151 150 ug
EUR 370

Human VEGF 121 AssayLite™ Antibody (APC Conjugate)

32562-05161 150 ug
EUR 370

Human VEGF 121 AssayLite™ Antibody (FITC Conjugate)

32562-05141 150 ug
EUR 370

Human VEGF 121 AssayLite™ Antibody (PerCP Conjugate)

32562-05171 150 ug
EUR 410

VEGF (121 a.a.), Vascular Endothelial Growth Factor-121 Human Recombinant Protein, His Tag

PROTP15692-8 Regular: 10ug
EUR 380.4
Description: Vascular Endothelial Growth Factor-121 Human Recombinant produced in E.Coli is a double, non-glycosylated, polypeptide chain (aa 207-327) containing a total of 142 amino acids and having a molecular mass of 16.3 kDa. ;The VEGF-121 is fused to a 20 amino acid His tag at N-terminus and purified by proprietary chromatographic techniques.

Recombinant Human VEGF-121 Isoform Protein, Untagged, E.coli-1mg

QP5207-1mg 1mg
EUR 4504.8

Recombinant Human VEGF-121 Isoform Protein, Untagged, E.coli-2ug

QP5207-2ug 2ug
EUR 163.2

Recombinant Human VEGF-121 Isoform Protein, Untagged, E.coli-10ug

QP5207-10ug 10ug
EUR 284.4

Recombinant Human VEGF-121 Isoform Protein, Untagged, E.coli-100ug

QP5207-100ug 100ug
EUR 654

Recombinant Human VEGF-121 Isoform Protein, Untagged, E.coli-500ug

QP5207-500ug 500ug
EUR 2786.4

Recombinant Mouse VEGF (121 a. a. ) Protein, Untagged, E.coli-1mg

QP13933-1mg 1mg
EUR 4287.6

Recombinant Mouse VEGF (121 a. a. ) Protein, Untagged, E.coli-2ug

QP13933-2ug 2ug
EUR 186

Recombinant Mouse VEGF (121 a. a. ) Protein, Untagged, E.coli-10ug

QP13933-10ug 10ug
EUR 241.2

VEGF Vascular Endothelial Growth Factor (121 a.a.) Human Recombinant Protein

PROTP15692-5 Regular: 10ug
EUR 380.4
Description: Vascular Endothelial Growth Factor-121 Human Recombinant produced in E.Coli is a double, non-glycosylated, polypeptide chain containing 121 amino acids and having a molecular mass of 28423 Dalton. VEGF121 circulates more freely than other VEGF forms, which bind more tightly with vascular heparin sulfates.;The VEGF-121 is purified by proprietary chromatographic techniques.

VEGF Vascular Endothelial Growth Factor (121 a.a.) Mouse Recombinant Protein

PROTQ00731-2 Regular: 10ug
EUR 380.4
Description: Vascular Endothelial Growth Factor-121 Mouse Recombinant produced in E.Coli is a homodimer, non-glycosylated, polypeptide chain containing 121 amino acids and having a molecular mass of 28.4 kDa.;Recombinant Mouse VEGF-121 is a truncated version of Murine VEGF-165.;The VEGF-121 is purified by proprietary chromatographic techniques.

VEGF (121a.a.) Human, Vascular Endothelial Growth Factor (121) Human Recombinant Protein, HEK

PROTP15692 Regular: 10ug
EUR 380.4
Description: Recombinant Human VEGF 121 amino acids, produced in HEK cells is a glycosylated 37kDa homodimer and 50kDa homotrimer.;The VEGF is purified by proprietary chromatographic techniques.

VEGF-A(VEGF/1063) Antibody

BNCAP1063-100 100uL
EUR 238.8
Description: Primary antibody against VEGF-A(VEGF/1063), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

VEGF-A(VEGF/1063) Antibody

BNCAP1063-500 500uL
EUR 652.8
Description: Primary antibody against VEGF-A(VEGF/1063), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

VEGF-A(VEGF/1063) Antibody

BNCH1063-100 100uL
EUR 238.8
Description: Primary antibody against VEGF-A(VEGF/1063), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

VEGF-A(VEGF/1063) Antibody

BNCH1063-500 500uL
EUR 652.8
Description: Primary antibody against VEGF-A(VEGF/1063), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

VEGF-A(VEGF/1063) Antibody

BNC701063-100 100uL
EUR 238.8
Description: Primary antibody against VEGF-A(VEGF/1063), CF770 conjugate, Concentration: 0.1mg/mL

VEGF-A(VEGF/1063) Antibody

BNC701063-500 500uL
EUR 652.8
Description: Primary antibody against VEGF-A(VEGF/1063), CF770 conjugate, Concentration: 0.1mg/mL

VEGF-A(VEGF/1063) Antibody

BNC811063-100 100uL
EUR 238.8
Description: Primary antibody against VEGF-A(VEGF/1063), CF680R conjugate, Concentration: 0.1mg/mL

VEGF-A(VEGF/1063) Antibody

BNC811063-500 500uL
EUR 652.8
Description: Primary antibody against VEGF-A(VEGF/1063), CF680R conjugate, Concentration: 0.1mg/mL

VEGF-A(VEGF/1063) Antibody

BNC801063-100 100uL
EUR 238.8
Description: Primary antibody against VEGF-A(VEGF/1063), CF680 conjugate, Concentration: 0.1mg/mL

VEGF-A(VEGF/1063) Antibody

BNC801063-500 500uL
EUR 652.8
Description: Primary antibody against VEGF-A(VEGF/1063), CF680 conjugate, Concentration: 0.1mg/mL

VEGF-A(VEGF/1063) Antibody

BNC551063-100 100uL
EUR 238.8
Description: Primary antibody against VEGF-A(VEGF/1063), CF555 conjugate, Concentration: 0.1mg/mL

VEGF-A(VEGF/1063) Antibody

BNC551063-500 500uL
EUR 652.8
Description: Primary antibody against VEGF-A(VEGF/1063), CF555 conjugate, Concentration: 0.1mg/mL

VEGF-A(VEGF/1063) Antibody

BNC431063-100 100uL
EUR 238.8
Description: Primary antibody against VEGF-A(VEGF/1063), CF543 conjugate, Concentration: 0.1mg/mL

VEGF-A(VEGF/1063) Antibody

BNC431063-500 500uL
EUR 652.8
Description: Primary antibody against VEGF-A(VEGF/1063), CF543 conjugate, Concentration: 0.1mg/mL

VEGF-A(VEGF/1063) Antibody

BNC611063-100 100uL
EUR 238.8
Description: Primary antibody against VEGF-A(VEGF/1063), CF660R conjugate, Concentration: 0.1mg/mL

VEGF-A(VEGF/1063) Antibody

BNC611063-500 500uL
EUR 652.8
Description: Primary antibody against VEGF-A(VEGF/1063), CF660R conjugate, Concentration: 0.1mg/mL

VEGF-A(VEGF/1063) Antibody

BNC051063-100 100uL
EUR 238.8
Description: Primary antibody against VEGF-A(VEGF/1063), CF405M conjugate, Concentration: 0.1mg/mL

VEGF-A(VEGF/1063) Antibody

BNC051063-500 500uL
EUR 652.8
Description: Primary antibody against VEGF-A(VEGF/1063), CF405M conjugate, Concentration: 0.1mg/mL

VEGF-A(VEGF/1063) Antibody

BNCA1063-250 250uL
EUR 459.6
Description: Primary antibody against VEGF-A(VEGF/1063), APC conjugate, Concentration: 0.1mg/mL

VEGF-A(VEGF/1063) Antibody

BNCP1063-250 250uL
EUR 459.6
Description: Primary antibody against VEGF-A(VEGF/1063), PerCP conjugate, Concentration: 0.1mg/mL

VEGF-A(VEGF/1063) Antibody

BNCR1063-250 250uL
EUR 459.6
Description: Primary antibody against VEGF-A(VEGF/1063), RPE conjugate, Concentration: 0.1mg/mL

VEGF/ Rat VEGF ELISA Kit

ELA-E0143r 96 Tests
EUR 1063.2

VEGF

E8ET1604-28 100ul
EUR 275
Description: Available in various conjugation types.

VEGF

GWB-400310 0.01 mg Ask for price

VEGF

cyt-241 2µg
EUR 60
Description: Recombinant Human Vascular Endothelial Growth Factor

VEGF

ant-125 0.5mg
EUR 275
Description: Mouse Anti Human Vascular Endothelial Growth Factor

VEGF

LF-PR009 10 ug
EUR 306
Description: VEGF protein

VEGF

MO15067 500 ug
EUR 1092

VEGF

PR15028 10 ug
EUR 553.2

VEGF

PA1080 100μg
EUR 260

VEGF

PA1080-M 100μg
EUR 290
Description: Polyclonal Antibodies Conjugated to Magnetic Beads

VEGF

PA1080-S 100μg
EUR 290
Description: Polyclonal Antibodies Conjugated to Sepharose Beads

VEGF-A(VEGF/1063), 1mg/mL

BNUM1063-50 50uL
EUR 396
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

VEGF-A(VEGF/1063), 0.2mg/mL

BNUB1063-100 100uL
EUR 225
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

VEGF-A(VEGF/1063), 0.2mg/mL

BNUB1063-500 500uL
EUR 485
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

VEGF Rabbit Polyclonal Antibody(VEGF)

RA20266-100ul 100 ul
EUR 298

VEGF Rabbit Polyclonal Antibody(VEGF)

RA20266-50ul 50 ul
EUR 198

VEGF Human, Vascular Endothelial Growth Factor (121 a.a.) Human Recombinant Protein, Sf9

PROTP15692-1 Regular: 10ug
EUR 380.4
Description: Vascular Endothelial Growth Factor-121 Human Recombinant produced in insect cells as an 18kDa homodimer, is a glycosylated, polypeptide chain containing 121 amino acids and having a molecular mass of approximately 36kDa.;VEGF121 circulates more freely than other VEGF forms, which bind more tightly with vascular heparin sulfates.;The VEGF-121 is purified by proprietary chromatographic techniques.

VEGF 162

PR15030 25 ug
EUR 553.2

VEGF 165

PR15031 50 ug
EUR 1477.2

VEGF 120

PR15033 5 ug
EUR 459.6

VEGF 164

PR15034 25 ug
EUR 1352.4

VEGF 164

PR15035 50 ug
EUR 1477.2

VEGF (121a.a.)

cyt-343 2µg
EUR 60
Description: Recombinant Human Vascular Endothelial Growth Factor (121)

VEGF-A/ Rat VEGF- A ELISA Kit

ELA-E1851r 96 Tests
EUR 1063.2

VEGF-B/ Rat VEGF- B ELISA Kit

ELA-E0144r 96 Tests
EUR 1063.2

VEGF-C/ Rat VEGF- C ELISA Kit

ELA-E0145r 96 Tests
EUR 1063.2

VEGF-D/ Rat VEGF- D ELISA Kit

ELA-E0146r 96 Tests
EUR 1063.2

VEGF D

cyt-045 2µg
EUR 60
Description: Recombinant Human Vascular Endothelial Growth Factor D

EG VEGF

cyt-338 5µg
EUR 60
Description: Recombinant Human Endocrine Gland Vascular Endothelial Growth Factor

VEGF, CF

PR15028CF 10 ug
EUR 553.2

VEGF, HEK

cyt-225 2µg
EUR 60
Description: Recombinant Human Vascular Endothelial Growth Factor, HEK