Cell Free DNA content material in tradition medium and mitochondrial DNA copy

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Comparative evaluation of cell-free DNA content material in tradition medium and mitochondrial DNA copy quantity in porcine parthenogenetically activated embryos

We examined the impact of ploidy on mitochondrial DNA (mtDNA) copy quantity in embryos and the quantity of cell-free mitochondrial and nucleic DNA content material (cf-mtDNA and cf-nDNA) in spent tradition medium (SCM).
  • Oocytes collected from the ovaries had been matured, activated, incubated in medium containing cycloheximide (CHX) or CHX and cytochalasin B (CB) for 4.5 h to supply haploid or diploid embryos (H-group and D-group embryos).
  • These embryos had been cultured for 7 days, and the blastocysts and SCM had been examined.
  • The quantity of mtDNA and nDNA was decided by real-time PCR. The speed of growth to the blastocyst stage was increased for the D-group than for the H-group.
  • Furthermore, D-group blastocysts had much less mtDNA in comparison with the H-group blastocysts.
  • After activation, the mitochondrial content material was fixed earlier than the blastocyst stage in D-group embryos, however elevated earlier in H-group embryos.
  • The quantity of cf-mtDNA within the SCM of D-group blastocysts was higher than that of H-group blastocysts. Nevertheless, when the cf-mtDNA within the SCM of two cell-stage embryos (day 2 post-activation) was examined, the quantity of cf-mtDNA was higher within the H-group than within the D-group embryos.
  • When D-group embryos had been cultured for 7 days, a big correlation was noticed between the full cell variety of blastocysts and cf-nDNA content material within the SCM.
  • Therefore, though cautious consideration is required relating to the time level for evaluating mtDNA content material within the embryos and SCM, this research demonstrates that mtDNA within the embryos and SCM was affected by the ploidy of the embryos.

Quick compressive lens-free tomography for 3D organic cell tradition imaging

We current a compressive lens-free method that performs tomographic imaging throughout a cubic millimeter-scale quantity from extremely sparse knowledge.

In contrast with present lens-free 3D microscopy methods, our technique requires an order of magnitude fewer multi-angle illuminations for tomographic reconstruction, resulting in a compact, cost-effective and scanning-free setup with a lowered knowledge acquisition time to allow high-throughput 3D imaging of dynamic organic processes. We apply a quick proximal gradient algorithm with composite regularization to deal with the ill-posed tomographic inverse downside.

Utilizing simulated knowledge, we present that the proposed technique can obtain a reconstruction pace ∼10× sooner than the state-of-the-art inverse downside strategy in 3D lens-free microscopy.

We experimentally validate the effectiveness of our technique by imaging a decision check chart and polystyrene beads, demonstrating its functionality to resolve micron-size options in each lateral and axial instructions.

Moreover, tomographic reconstruction outcomes of neuronspheres and intestinal organoids reveal the potential of this 3D imaging method for high-resolution and high-throughput organic purposes.

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HY-P7265 50ug
EUR 533

MIP-4 Recombinant Protein
40-385-0002mg 0.002 mg
EUR 259.25
Description: MIP-4 is a CC chemokine that is expressed in lymph nodes, lungs, placenta and bone marrow. MIP-4's primary receptor is unknown. MIP-4 chemoattracts lymphocytes, and has been shown to exert activity on both CD4+ and CD8+ T cells. Recombinant human MIP-4 is a 7.8 kDa protein containing 69 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

MIP-4 Recombinant Protein
40-385-001mg 0.01 mg
EUR 364.25
Description: MIP-4 is a CC chemokine that is expressed in lymph nodes, lungs, placenta and bone marrow. MIP-4's primary receptor is unknown. MIP-4 chemoattracts lymphocytes, and has been shown to exert activity on both CD4+ and CD8+ T cells. Recombinant human MIP-4 is a 7.8 kDa protein containing 69 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

Sheep MIP-4 ELISA Kit
ESM0021 96Tests
EUR 521

MIP-4, PARC, CCL18, human
RC315-29 2ug
EUR 104.38

Anserini MIP-4 ELISA Kit
EAM0021 96Tests
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Rat MIP-4 ELISA Kit
ERM0021 96Tests
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Rabbit MIP-4 ELISA Kit
ERTM0021 96Tests
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Monkey MIP-4 ELISA Kit
EMKM0021 96Tests
EUR 521

Mouse MIP-4 ELISA Kit
EMM0021 96Tests
EUR 521

Porcine MIP-4 ELISA Kit
EPM0021 96Tests
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Human MIP-4 ELISA Kit
EHM0021 96Tests
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Chicken MIP-4 ELISA Kit
ECKM0021 96Tests
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Canine MIP-4 ELISA Kit
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Bovine MIP-4 ELISA Kit
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Goat MIP-4 ELISA Kit
EGTM0021 96Tests
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MIP-4 (PARC), human recombinant
4257-10
EUR 338

MIP-4 (PARC), human recombinant
4257-1000
EUR 5917

IL-4 Interleukin 4 Human Recombinant Protein, Yeast
PROTP05112-4 Regular: 10ug
EUR 317
Description: Interleukin-4 Human Recombinant produced in yeast is a single, glycosylated polypeptide chain containing 129 amino acids.;The IL-4 is purified by proprietary chromatographic techniques.

Recombinant Human MIP-4 (CCL18) Protein
PROTP55774-2 10ug
EUR 317
Description: MIP-4 is a CC chemokine that is expressed in lymph nodes, lungs, placenta and bone marrow. MIP-4's primary receptor is unknown. MIP-4 chemoattracts lymphocytes, and has been shown to exert activity on both CD4+ and CD8+ T cells. Recombinant human MIP-4 is a 7.8 kDa protein containing 68 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

Guinea Pig MIP-4 ELISA Kit
EGM0021 96Tests
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Individual Reaction Mix 4
G065-4 200 reactions
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Adipose Tissue from Lean and Overweight Mice Induces a Mesenchymal to Epithelial Transition-Like Impact in Triple Unfavourable Breast Cancers Cells Grown in 3-Dimensional Tradition

Breast most cancers is the second main reason behind cancer-related mortality amongst ladies globally with weight problems being one threat issue. Overweight breast most cancers sufferers have a minimum of a 30% elevated threat of loss of life from breast most cancers in comparison with non-obese breast most cancers sufferers as a result of they current with bigger tumors and customarily have elevated charges of metastasis.
Furthermore, overweight breast most cancers sufferers reply extra poorly to therapy in comparison with non-obese sufferers, significantly pre-menopausal ladies identified with triple detrimental breast most cancers (TNBC).
To assist perceive the molecular mechanisms underlying the elevated metastasis related to weight problems, we beforehand established a three-dimensional tradition system that allows the co-culture of adipocytes and TNBC cells in a way that mimics an in vivo milieu.
Utilizing this technique, we display that white adipose tissue from each lean and overweight mice can induce a partial mesenchymal-to-epithelial transition (MET).
Triple detrimental breast most cancers cells undertake an epithelial morphology and have an elevated expression of some epithelial markers, however they keep the expression of mesenchymal markers, furnishing the breast most cancers cells with hybrid properties which are related to extra aggressive tumors.
Thus, these knowledge counsel that adipose tissue has the potential to promote secondary tumor formation in lean and overweight ladies. Additional work is required to find out if focusing on the partial MET induced by adipose tissue may scale back metastasis.

Procalcitonin Antibody
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abx023817-1mg 1 mg
EUR 878

Procalcitonin Protein
20-abx262327
  • EUR 328.00
  • EUR 5993.00
  • EUR 230.00
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  • 1 mg
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20-abx262966
  • EUR 328.00
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6640-100
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Procalcitonin Antibody
6640-30T
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Procalcitonin antibody
10-7941 500 ug
EUR 338
Description: Mouse monoclonal Procalcitonin antibody

Procalcitonin antibody
10-7942 500 ug
EUR 338
Description: Mouse monoclonal Procalcitonin antibody

Procalcitonin antibody
10-7943 500 ug
EUR 338
Description: Mouse monoclonal Procalcitonin antibody

Procalcitonin antibody
10-7944 500 ug
EUR 338
Description: Mouse monoclonal Procalcitonin antibody

Procalcitonin protein
30-1379 500 ug
EUR 392
Description: Recombinant Procalcitonin protein

Procalcitonin protein
30-1908 100 ug
EUR 931
Description: Purified Recombinant Procalcitonin protein

Procalcitonin protein
30R-2425 10 ug
EUR 328
Description: Purified recombinant Human Procalcitonin protein

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