Cell Free DNA content material in tradition medium and mitochondrial DNA copy

pharmacodane

Comparative evaluation of cell-free DNA content material in tradition medium and mitochondrial DNA copy quantity in porcine parthenogenetically activated embryos

We examined the impact of ploidy on mitochondrial DNA (mtDNA) copy quantity in embryos and the quantity of cell-free mitochondrial and nucleic DNA content material (cf-mtDNA and cf-nDNA) in spent tradition medium (SCM).
  • Oocytes collected from the ovaries had been matured, activated, incubated in medium containing cycloheximide (CHX) or CHX and cytochalasin B (CB) for 4.5 h to supply haploid or diploid embryos (H-group and D-group embryos).
  • These embryos had been cultured for 7 days, and the blastocysts and SCM had been examined.
  • The quantity of mtDNA and nDNA was decided by real-time PCR. The speed of growth to the blastocyst stage was increased for the D-group than for the H-group.
  • Furthermore, D-group blastocysts had much less mtDNA in comparison with the H-group blastocysts.
  • After activation, the mitochondrial content material was fixed earlier than the blastocyst stage in D-group embryos, however elevated earlier in H-group embryos.
  • The quantity of cf-mtDNA within the SCM of D-group blastocysts was higher than that of H-group blastocysts. Nevertheless, when the cf-mtDNA within the SCM of two cell-stage embryos (day 2 post-activation) was examined, the quantity of cf-mtDNA was higher within the H-group than within the D-group embryos.
  • When D-group embryos had been cultured for 7 days, a big correlation was noticed between the full cell variety of blastocysts and cf-nDNA content material within the SCM.
  • Therefore, though cautious consideration is required relating to the time level for evaluating mtDNA content material within the embryos and SCM, this research demonstrates that mtDNA within the embryos and SCM was affected by the ploidy of the embryos.

Quick compressive lens-free tomography for 3D organic cell tradition imaging

We current a compressive lens-free method that performs tomographic imaging throughout a cubic millimeter-scale quantity from extremely sparse knowledge.

In contrast with present lens-free 3D microscopy methods, our technique requires an order of magnitude fewer multi-angle illuminations for tomographic reconstruction, resulting in a compact, cost-effective and scanning-free setup with a lowered knowledge acquisition time to allow high-throughput 3D imaging of dynamic organic processes. We apply a quick proximal gradient algorithm with composite regularization to deal with the ill-posed tomographic inverse downside.

Utilizing simulated knowledge, we present that the proposed technique can obtain a reconstruction pace ∼10× sooner than the state-of-the-art inverse downside strategy in 3D lens-free microscopy.

We experimentally validate the effectiveness of our technique by imaging a decision check chart and polystyrene beads, demonstrating its functionality to resolve micron-size options in each lateral and axial instructions.

Moreover, tomographic reconstruction outcomes of neuronspheres and intestinal organoids reveal the potential of this 3D imaging method for high-resolution and high-throughput organic purposes.

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rHu MIP-1-alpha

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rHu MIP-1-alpha

AK8250-0020 20µg Ask for price

rHu MIP-1-alpha

AK8250-0100 100µg Ask for price

rHu MIP-1-alpha

AK8250-1000 1mg Ask for price

rHu MIP-1-beta

AK8264-0002 2µg Ask for price

rHu MIP-1-beta

AK8264-0010 10µg Ask for price

rHu MIP-1-beta

AK8264-0100 100µg Ask for price

rHu MIP-1-beta

AK8264-1000 1mg Ask for price

rHu MIP-3-alpha

AK8271-0005 5µg Ask for price

rHu MIP-3-alpha

AK8271-0020 20µg Ask for price

rHu MIP-3-alpha

AK8271-0100 100µg Ask for price

rHu MIP-3-alpha

AK8271-1000 1mg Ask for price

rHu IL-4

AK8329-0005 5µg Ask for price

rHu IL-4

AK8329-0020 20µg Ask for price

rHu IL-4

AK8329-0100 100µg Ask for price

rHu IL-4

AK8329-1000 1mg Ask for price

rHu MCP-4

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rHu MCP-4

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rHu MCP-4

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rHu MCP-4

AK8332-1000 1mg Ask for price

rHu FGF-4

AK9698-0005 5µg Ask for price

rHu FGF-4

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rHu FGF-4

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rHu FGF-4

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rHu IL-4 (cGMP)

AK9839-0000 1mg Ask for price

rHu BMP-4 (CHO)

AK9996-0005 5µg Ask for price

rHu BMP-4 (CHO)

AK9996-0020 20µg Ask for price

rHu BMP-4 (CHO)

AK9996-0100 100µg Ask for price

rHu BMP-4 (CHO)

AK9996-1000 1mg Ask for price

MIP-4 Recombinant Protein

40-385-0002mg 0.002 mg
EUR 311.1
Description: MIP-4 is a CC chemokine that is expressed in lymph nodes, lungs, placenta and bone marrow. MIP-4's primary receptor is unknown. MIP-4 chemoattracts lymphocytes, and has been shown to exert activity on both CD4+ and CD8+ T cells. Recombinant human MIP-4 is a 7.8 kDa protein containing 69 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

MIP-4 Recombinant Protein

40-385-001mg 0.01 mg
EUR 437.1
Description: MIP-4 is a CC chemokine that is expressed in lymph nodes, lungs, placenta and bone marrow. MIP-4's primary receptor is unknown. MIP-4 chemoattracts lymphocytes, and has been shown to exert activity on both CD4+ and CD8+ T cells. Recombinant human MIP-4 is a 7.8 kDa protein containing 69 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

MIP-4/CCL18, Human

HY-P7265 50ug
EUR 639.6

Recombinant Human Interleukin-4 (rHu IL-4)

AK9839-0010 10ug
EUR 780

Recombinant Human Interleukin-4 (rHu IL-4)

AK9839-0030 30ug
EUR 1356

Recombinant Human Interleukin-4 (rHu IL-4)

AK9839-0100 100ug
EUR 2040

Sheep MIP-4 ELISA Kit

ESM0021 96Tests
EUR 625.2

Human MIP-4 ELISA Kit

EHM0021 96Tests
EUR 625.2

Porcine MIP-4 ELISA Kit

EPM0021 96Tests
EUR 625.2

Monkey MIP-4 ELISA Kit

EMKM0021 96Tests
EUR 625.2

Mouse MIP-4 ELISA Kit

EMM0021 96Tests
EUR 625.2

Rat MIP-4 ELISA Kit

ERM0021 96Tests
EUR 625.2

Rabbit MIP-4 ELISA Kit

ERTM0021 96Tests
EUR 625.2

Goat MIP-4 ELISA Kit

EGTM0021 96Tests
EUR 625.2

Bovine MIP-4 ELISA Kit

EBM0021 96Tests
EUR 625.2

Anserini MIP-4 ELISA Kit

EAM0021 96Tests
EUR 625.2

Chicken MIP-4 ELISA Kit

ECKM0021 96Tests
EUR 625.2

Canine MIP-4 ELISA Kit

ECM0021 96Tests
EUR 625.2

MIP-4, PARC, CCL18, human

RC315-29 2ug
EUR 125.26

MIP-4 (PARC), human recombinant

4257-10 each
EUR 405.6

MIP-4 (PARC), human recombinant

4257-1000 each
EUR 7100.4

Guinea Pig MIP-4 ELISA Kit

EGM0021 96Tests
EUR 625.2

Recombinant Human MIP-4 (CCL18) Protein

PROTP55774-2 10ug
EUR 380.4
Description: MIP-4 is a CC chemokine that is expressed in lymph nodes, lungs, placenta and bone marrow. MIP-4's primary receptor is unknown. MIP-4 chemoattracts lymphocytes, and has been shown to exert activity on both CD4+ and CD8+ T cells. Recombinant human MIP-4 is a 7.8 kDa protein containing 68 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

IL-4 Interleukin 4 Human Recombinant Protein, Yeast

PROTP05112-4 Regular: 10ug
EUR 380.4
Description: Interleukin-4 Human Recombinant produced in yeast is a single, glycosylated polypeptide chain containing 129 amino acids.;The IL-4 is purified by proprietary chromatographic techniques.

Individual Reaction Mix 4

G065-4 200 reactions
EUR 200.4

rHu Insulin

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rHu Insulin

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rHu Insulin

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rHu aFGF

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rHu aFGF

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rHu aFGF

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rHu aFGF

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rHu MCSF

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rHu MCSF

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rHu CTGF

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rHu CTGF

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rHu MIF

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rHu MIF

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rHu MIF

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rHu MIF

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rHu Myostatin

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rHu Myostatin

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rHu Myostatin

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rHu Myostatin

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Adipose Tissue from Lean and Overweight Mice Induces a Mesenchymal to Epithelial Transition-Like Impact in Triple Unfavourable Breast Cancers Cells Grown in 3-Dimensional Tradition

Breast most cancers is the second main reason behind cancer-related mortality amongst ladies globally with weight problems being one threat issue. Overweight breast most cancers sufferers have a minimum of a 30% elevated threat of loss of life from breast most cancers in comparison with non-obese breast most cancers sufferers as a result of they current with bigger tumors and customarily have elevated charges of metastasis.
Furthermore, overweight breast most cancers sufferers reply extra poorly to therapy in comparison with non-obese sufferers, significantly pre-menopausal ladies identified with triple detrimental breast most cancers (TNBC).
To assist perceive the molecular mechanisms underlying the elevated metastasis related to weight problems, we beforehand established a three-dimensional tradition system that allows the co-culture of adipocytes and TNBC cells in a way that mimics an in vivo milieu.
Utilizing this technique, we display that white adipose tissue from each lean and overweight mice can induce a partial mesenchymal-to-epithelial transition (MET).
Triple detrimental breast most cancers cells undertake an epithelial morphology and have an elevated expression of some epithelial markers, however they keep the expression of mesenchymal markers, furnishing the breast most cancers cells with hybrid properties which are related to extra aggressive tumors.
Thus, these knowledge counsel that adipose tissue has the potential to promote secondary tumor formation in lean and overweight ladies. Additional work is required to find out if focusing on the partial MET induced by adipose tissue may scale back metastasis.

Procalcitonin protein

30-1908 100 ug
EUR 1117.2
Description: Purified Recombinant Procalcitonin protein

Procalcitonin antibody

10-7941 500 ug
EUR 405.6
Description: Mouse monoclonal Procalcitonin antibody

Procalcitonin antibody

10-7942 500 ug
EUR 405.6
Description: Mouse monoclonal Procalcitonin antibody

Procalcitonin antibody

10-7943 500 ug
EUR 405.6
Description: Mouse monoclonal Procalcitonin antibody

Procalcitonin antibody

10-7944 500 ug
EUR 405.6
Description: Mouse monoclonal Procalcitonin antibody

Procalcitonin Antibody

20-abx121310
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

Procalcitonin Antibody

abx023816-1mg 1 mg
EUR 1053.6

Procalcitonin Antibody

abx023817-1mg 1 mg
EUR 1053.6

Procalcitonin Protein

abx061017-100ug 100 ug
EUR 2298

Procalcitonin Protein

20-abx262327
  • EUR 393.60
  • EUR 7191.60
  • EUR 276.00
  • 10 ug
  • 1 mg
  • 2 µg

Procalcitonin Protein

20-abx262966
  • EUR 393.60
  • EUR 7676.40
  • EUR 276.00
  • 10 ug
  • 1 mg
  • 2 µg

Procalcitonin Protein

20-abx262989
  • EUR 393.60
  • EUR 8010.00
  • EUR 276.00
  • 10 ug
  • 1 mg
  • 2 µg

Procalcitonin Antibody

6640-100 each
EUR 326.4

Procalcitonin Antibody

6640-30T each
EUR 175.2

rHu Insulin

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rHu Insulin

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rHu Insulin

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rHu aFGF

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rHu aFGF

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rHu aFGF

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rHu aFGF

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rHu MCSF

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rHu MCSF

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rHu MCSF

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rHu MCSF

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rHu CTGF

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rHu CTGF

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rHu CTGF

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rHu CTGF

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rHu MIF

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rHu MIF

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rHu MIF

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rHu MIF

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rHu Myostatin

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rHu Myostatin

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rHu Myostatin

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rHu Myostatin

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rHu Leptin

AK8265-0200 200µg Ask for price

rHu Leptin

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rHu Leptin

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rHu RANTES

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rHu RANTES

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rHu BDNF

AK8286-0002 2µg Ask for price

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rHu BDNF

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rHu CNTF

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rHu CNTF

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rHu EGF

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rHu GDNF

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rHu OSM

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rHu BAFF

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rHu Artemin

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rHu Omentin

AK9083-0002 2µg Ask for price

rHu Omentin

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rHu Omentin

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rHu Resistin

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rHu SHH

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rHu TPO

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rHu TPO

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rHu TRAIL

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rHu TRAIL

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rHu TRAIL

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rHu TSLP

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rHu TSLP

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rHu TSLP

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rHu Chemerin

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