Comparative evaluation of cell-free DNA content material in tradition medium and mitochondrial DNA copy quantity in porcine parthenogenetically activated embryos
We examined the impact of ploidy on mitochondrial DNA (mtDNA) copy quantity in embryos and the quantity of cell-free mitochondrial and nucleic DNA content material (cf-mtDNA and cf-nDNA) in spent tradition medium (SCM).
- Oocytes collected from the ovaries had been matured, activated, incubated in medium containing cycloheximide (CHX) or CHX and cytochalasin B (CB) for 4.5 h to supply haploid or diploid embryos (H-group and D-group embryos).
- These embryos had been cultured for 7 days, and the blastocysts and SCM had been examined.
- The quantity of mtDNA and nDNA was decided by real-time PCR. The speed of growth to the blastocyst stage was increased for the D-group than for the H-group.
- Furthermore, D-group blastocysts had much less mtDNA in comparison with the H-group blastocysts.
- After activation, the mitochondrial content material was fixed earlier than the blastocyst stage in D-group embryos, however elevated earlier in H-group embryos.
- The quantity of cf-mtDNA within the SCM of D-group blastocysts was higher than that of H-group blastocysts. Nevertheless, when the cf-mtDNA within the SCM of two cell-stage embryos (day 2 post-activation) was examined, the quantity of cf-mtDNA was higher within the H-group than within the D-group embryos.
- When D-group embryos had been cultured for 7 days, a big correlation was noticed between the full cell variety of blastocysts and cf-nDNA content material within the SCM.
- Therefore, though cautious consideration is required relating to the time level for evaluating mtDNA content material within the embryos and SCM, this research demonstrates that mtDNA within the embryos and SCM was affected by the ploidy of the embryos.
Quick compressive lens-free tomography for 3D organic cell tradition imaging
We current a compressive lens-free method that performs tomographic imaging throughout a cubic millimeter-scale quantity from extremely sparse knowledge.
In contrast with present lens-free 3D microscopy methods, our technique requires an order of magnitude fewer multi-angle illuminations for tomographic reconstruction, resulting in a compact, cost-effective and scanning-free setup with a lowered knowledge acquisition time to allow high-throughput 3D imaging of dynamic organic processes. We apply a quick proximal gradient algorithm with composite regularization to deal with the ill-posed tomographic inverse downside.
Utilizing simulated knowledge, we present that the proposed technique can obtain a reconstruction pace ∼10× sooner than the state-of-the-art inverse downside strategy in 3D lens-free microscopy.
We experimentally validate the effectiveness of our technique by imaging a decision check chart and polystyrene beads, demonstrating its functionality to resolve micron-size options in each lateral and axial instructions.
Moreover, tomographic reconstruction outcomes of neuronspheres and intestinal organoids reveal the potential of this 3D imaging method for high-resolution and high-throughput organic purposes.
pharmacodane
 rHu MIP-3-beta |
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| AK8232-0005 | Akron Biotech | 5µg | Ask for price |
rHu MIP-3-beta |
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| AK8232-0020 | Akron Biotech | 20µg | Ask for price |
rHu MIP-3-beta |
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| AK8232-0100 | Akron Biotech | 100µg | Ask for price |
rHu MIP-3-beta |
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| AK8232-1000 | Akron Biotech | 1mg | Ask for price |
 rHu MIP-1-alpha |
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| AK8250-0005 | Akron Biotech | 5µg | Ask for price |
rHu MIP-1-alpha |
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| AK8250-0020 | Akron Biotech | 20µg | Ask for price |
rHu MIP-1-alpha |
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| AK8250-0100 | Akron Biotech | 100µg | Ask for price |
rHu MIP-1-alpha |
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| AK8250-1000 | Akron Biotech | 1mg | Ask for price |
 rHu MIP-1-beta |
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| AK8264-0002 | Akron Biotech | 2µg | Ask for price |
rHu MIP-1-beta |
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| AK8264-0010 | Akron Biotech | 10µg | Ask for price |
rHu MIP-1-beta |
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| AK8264-0100 | Akron Biotech | 100µg | Ask for price |
rHu MIP-1-beta |
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| AK8264-1000 | Akron Biotech | 1mg | Ask for price |
 rHu MIP-3-alpha |
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| AK8271-0005 | Akron Biotech | 5µg | Ask for price |
rHu MIP-3-alpha |
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| AK8271-0020 | Akron Biotech | 20µg | Ask for price |
rHu MIP-3-alpha |
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| AK8271-0100 | Akron Biotech | 100µg | Ask for price |
rHu MIP-3-alpha |
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| AK8271-1000 | Akron Biotech | 1mg | Ask for price |
 rHu IL-4 |
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| AK8329-0005 | Akron Biotech | 5µg | Ask for price |
rHu IL-4 |
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| AK8329-0020 | Akron Biotech | 20µg | Ask for price |
rHu IL-4 |
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| AK8329-0100 | Akron Biotech | 100µg | Ask for price |
rHu IL-4 |
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| AK8329-1000 | Akron Biotech | 1mg | Ask for price |
 rHu MCP-4 |
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| AK8332-0005 | Akron Biotech | 5µg | Ask for price |
rHu MCP-4 |
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| AK8332-0020 | Akron Biotech | 20µg | Ask for price |
rHu MCP-4 |
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| AK8332-0100 | Akron Biotech | 100µg | Ask for price |
rHu MCP-4 |
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| AK8332-1000 | Akron Biotech | 1mg | Ask for price |
 rHu FGF-4 |
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| AK9698-0005 | Akron Biotech | 5µg | Ask for price |
rHu FGF-4 |
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| AK9698-0025 | Akron Biotech | 25µg | Ask for price |
rHu FGF-4 |
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| AK9698-0100 | Akron Biotech | 100µg | Ask for price |
rHu FGF-4 |
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| AK9698-1000 | Akron Biotech | 1mg | Ask for price |
) rHu IL-4 (cGMP) |
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| AK9839-0000 | Akron Biotech | 1mg | Ask for price |
rHu IL-4 (cGMP) |
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| AK9839-0100 | Akron Biotech | 100ug | Ask for price |
) rHu BMP-4 (CHO) |
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| AK9996-0005 | Akron Biotech | 5µg | Ask for price |
rHu BMP-4 (CHO) |
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| AK9996-0020 | Akron Biotech | 20µg | Ask for price |
rHu BMP-4 (CHO) |
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| AK9996-0100 | Akron Biotech | 100µg | Ask for price |
rHu BMP-4 (CHO) |
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| AK9996-1000 | Akron Biotech | 1mg | Ask for price |
 MIP-4/CCL18, Human |
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| HY-P7265 | MedChemExpress | 50ug | EUR 533.00 |
, human recombinant) MIP-4 (PARC), human recombinant |
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| 4257-10 | Biovision | EUR 338.00 | |
MIP-4 (PARC), human recombinant |
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| 4257-1000 | Biovision | EUR 5917.00 | |
 Human MIP-4 ELISA Kit |
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| EHM0021 | Abclonal | 96Tests | EUR 521.00 |
 Bovine MIP-4 ELISA Kit |
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| EBM0021 | Abclonal | 96Tests | EUR 521.00 |
 Chicken MIP-4 ELISA Kit |
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| ECKM0021 | Abclonal | 96Tests | EUR 521.00 |
 Anserini MIP-4 ELISA Kit |
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| EAM0021 | Abclonal | 96Tests | EUR 521.00 |
 Canine MIP-4 ELISA Kit |
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| ECM0021 | Abclonal | 96Tests | EUR 521.00 |
 Goat MIP-4 ELISA Kit |
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| EGTM0021 | Abclonal | 96Tests | EUR 521.00 |
 Porcine MIP-4 ELISA Kit |
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| EPM0021 | Abclonal | 96Tests | EUR 521.00 |
 Sheep MIP-4 ELISA Kit |
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| ESM0021 | Abclonal | 96Tests | EUR 521.00 |
 Rat MIP-4 ELISA Kit |
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| ERM0021 | Abclonal | 96Tests | EUR 521.00 |
 Rabbit MIP-4 ELISA Kit |
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| ERTM0021 | Abclonal | 96Tests | EUR 521.00 |
 Monkey MIP-4 ELISA Kit |
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| EMKM0021 | Abclonal | 96Tests | EUR 521.00 |
 Mouse MIP-4 ELISA Kit |
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| EMM0021 | Abclonal | 96Tests | EUR 521.00 |
 MIP-4, PARC, CCL18, human |
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| RC315-29 | Bio Basic | 2ug | EUR 104.38 |
|
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 Guinea Pig MIP-4 ELISA Kit |
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| EGM0021 | Abclonal | 96Tests | EUR 521.00 |
 Protein) Recombinant Human MIP-4 (CCL18) Protein |
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| PROTP55774-2 | BosterBio | 10ug | EUR 317.00 |
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Description: MIP-4 is a CC chemokine that is expressed in lymph nodes, lungs, placenta and bone marrow. MIP-4's primary receptor is unknown. MIP-4 chemoattracts lymphocytes, and has been shown to exert activity on both CD4+ and CD8+ T cells. Recombinant human MIP-4 is a 7.8 kDa protein containing 68 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines. |
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 IL-4 Interleukin 4 Human Recombinant Protein, Yeast |
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| PROTP05112-4 | BosterBio | Regular: 10ug | EUR 317.00 |
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Description: Interleukin-4 Human Recombinant produced in yeast is a single, glycosylated polypeptide chain containing 129 amino acids.;The IL-4 is purified by proprietary chromatographic techniques. |
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 Individual Reaction Mix 4 |
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| G065-4 | ABM | 200 reactions | EUR 167.00 |
 rHu Insulin |
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| AK8211-0025 | Akron Biotech | 25mg | Ask for price |
rHu Insulin |
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| AK8211-0250 | Akron Biotech | 250mg | Ask for price |
rHu Insulin |
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| AK8211-1000 | Akron Biotech | 1g | Ask for price |
 rHu aFGF |
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| AK8230-0010 | Akron Biotech | 10µg | Ask for price |
rHu aFGF |
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| AK8230-0050 | Akron Biotech | 50µg | Ask for price |
rHu aFGF |
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| AK8230-0100 | Akron Biotech | 100µg | Ask for price |
rHu aFGF |
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| AK8230-1000 | Akron Biotech | 1mg | Ask for price |
 rHu MCSF |
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| AK8238-0002 | Akron Biotech | 2µg | Ask for price |
rHu MCSF |
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| AK8238-0010 | Akron Biotech | 10µg | Ask for price |
rHu MCSF |
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| AK8238-0100 | Akron Biotech | 100µg | Ask for price |
rHu MCSF |
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| AK8238-1000 | Akron Biotech | 1mg | Ask for price |
 rHu CTGF |
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| AK8240-0005 | Akron Biotech | 5µg | Ask for price |
rHu CTGF |
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| AK8240-0020 | Akron Biotech | 20µg | Ask for price |
rHu CTGF |
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| AK8240-0100 | Akron Biotech | 100µg | Ask for price |
rHu CTGF |
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| AK8240-1000 | Akron Biotech | 1mg | Ask for price |
 rHu MIF |
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| AK8255-0005 | Akron Biotech | 5µg | Ask for price |
rHu MIF |
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| AK8255-0025 | Akron Biotech | 25µg | Ask for price |
rHu MIF |
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| AK8255-0100 | Akron Biotech | 100µg | Ask for price |
rHu MIF |
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| AK8255-1000 | Akron Biotech | 1mg | Ask for price |
 rHu Myostatin |
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| AK8256-0002 | Akron Biotech | 2µg | Ask for price |
rHu Myostatin |
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| AK8256-0010 | Akron Biotech | 10µg | Ask for price |
rHu Myostatin |
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| AK8256-0100 | Akron Biotech | 100µg | Ask for price |
rHu Myostatin |
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| AK8256-1000 | Akron Biotech | 1mg | Ask for price |
Adipose Tissue from Lean and Overweight Mice Induces a Mesenchymal to Epithelial Transition-Like Impact in Triple Unfavourable Breast Cancers Cells Grown in 3-Dimensional Tradition
Breast most cancers is the second main reason behind cancer-related mortality amongst ladies globally with weight problems being one threat issue. Overweight breast most cancers sufferers have a minimum of a 30% elevated threat of loss of life from breast most cancers in comparison with non-obese breast most cancers sufferers as a result of they current with bigger tumors and customarily have elevated charges of metastasis.
Furthermore, overweight breast most cancers sufferers reply extra poorly to therapy in comparison with non-obese sufferers, significantly pre-menopausal ladies identified with triple detrimental breast most cancers (TNBC).
To assist perceive the molecular mechanisms underlying the elevated metastasis related to weight problems, we beforehand established a three-dimensional tradition system that allows the co-culture of adipocytes and TNBC cells in a way that mimics an in vivo milieu.
Utilizing this technique, we display that white adipose tissue from each lean and overweight mice can induce a partial mesenchymal-to-epithelial transition (MET).
Triple detrimental breast most cancers cells undertake an epithelial morphology and have an elevated expression of some epithelial markers, however they keep the expression of mesenchymal markers, furnishing the breast most cancers cells with hybrid properties which are related to extra aggressive tumors.
Thus, these knowledge counsel that adipose tissue has the potential to promote secondary tumor formation in lean and overweight ladies. Additional work is required to find out if focusing on the partial MET induced by adipose tissue may scale back metastasis.
 Procalcitonin |
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| AT215 | Unibiotest | 1mg | EUR 1114.00 |
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Procalcitonin |
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| AG215 | Unibiotest | 1 mg | EUR 523.00 |
 Procalcitonin protein |
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| 30R-2425 | Fitzgerald | 10 ug | EUR 328.00 |
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Description: Purified recombinant Human Procalcitonin protein |
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|
Procalcitonin protein |
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| 30-1379 | Fitzgerald | 500 ug | EUR 392.00 |
|
Description: Recombinant Procalcitonin protein |
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|
Procalcitonin protein |
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| 30-1908 | Fitzgerald | 100 ug | EUR 931.00 |
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Description: Purified Recombinant Procalcitonin protein |
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 Procalcitonin Antibody |
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| 6640-100 | Biovision | EUR 272.00 | |
|
Procalcitonin Antibody |
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| 6640-30T | Biovision | EUR 146.00 | |
 Procalcitonin antibody |
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| 10-7941 | Fitzgerald | 500 ug | EUR 338.00 |
|
Description: Mouse monoclonal Procalcitonin antibody |
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|
Procalcitonin antibody |
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| 10-7942 | Fitzgerald | 500 ug | EUR 338.00 |
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Description: Mouse monoclonal Procalcitonin antibody |
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|
Procalcitonin antibody |
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| 10-7943 | Fitzgerald | 500 ug | EUR 338.00 |
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Description: Mouse monoclonal Procalcitonin antibody |
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Procalcitonin antibody |
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| 10-7944 | Fitzgerald | 500 ug | EUR 338.00 |
|
Description: Mouse monoclonal Procalcitonin antibody |
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|
Procalcitonin Antibody |
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| abx023816-1mg | Abbexa | 1 mg | EUR 878.00 |
|
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|
Procalcitonin Antibody |
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| abx023817-1mg | Abbexa | 1 mg | EUR 878.00 |
|
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|
Procalcitonin Antibody |
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| 20-abx121310 | Abbexa |
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|
|
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 Procalcitonin Protein |
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| abx061017-100ug | Abbexa | 100 ug | EUR 1915.00 |
|
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|
Procalcitonin Protein |
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| 20-abx262327 | Abbexa |
|
|
|
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|
Procalcitonin Protein |
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| 20-abx262966 | Abbexa |
|
|
|
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|
Procalcitonin Protein |
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| 20-abx262989 | Abbexa |
|
|
|
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rHu Insulin |
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| AK8211-0025 | Akron Biotech | 25mg | Ask for price |
|
rHu Insulin |
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| AK8211-0250 | Akron Biotech | 250mg | Ask for price |
|
rHu Insulin |
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| AK8211-1000 | Akron Biotech | 1g | Ask for price |
|
rHu aFGF |
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| AK8230-0010 | Akron Biotech | 10µg | Ask for price |
|
rHu aFGF |
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| AK8230-0050 | Akron Biotech | 50µg | Ask for price |
|
rHu aFGF |
|||
| AK8230-0100 | Akron Biotech | 100µg | Ask for price |
|
rHu aFGF |
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| AK8230-1000 | Akron Biotech | 1mg | Ask for price |
|
rHu MCSF |
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| AK8238-0002 | Akron Biotech | 2µg | Ask for price |
|
rHu MCSF |
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| AK8238-0010 | Akron Biotech | 10µg | Ask for price |
|
rHu MCSF |
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| AK8238-0100 | Akron Biotech | 100µg | Ask for price |
|
rHu MCSF |
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| AK8238-1000 | Akron Biotech | 1mg | Ask for price |
|
rHu CTGF |
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| AK8240-0005 | Akron Biotech | 5µg | Ask for price |
|
rHu CTGF |
|||
| AK8240-0020 | Akron Biotech | 20µg | Ask for price |
|
rHu CTGF |
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| AK8240-0100 | Akron Biotech | 100µg | Ask for price |
|
rHu CTGF |
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| AK8240-1000 | Akron Biotech | 1mg | Ask for price |
|
rHu MIF |
|||
| AK8255-0005 | Akron Biotech | 5µg | Ask for price |
|
rHu MIF |
|||
| AK8255-0025 | Akron Biotech | 25µg | Ask for price |
|
rHu MIF |
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| AK8255-0100 | Akron Biotech | 100µg | Ask for price |
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rHu MIF |
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| AK8255-1000 | Akron Biotech | 1mg | Ask for price |
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rHu Myostatin |
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| AK8256-0002 | Akron Biotech | 2µg | Ask for price |
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rHu Myostatin |
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| AK8256-0010 | Akron Biotech | 10µg | Ask for price |
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rHu Myostatin |
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| AK8256-0100 | Akron Biotech | 100µg | Ask for price |
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rHu Myostatin |
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| AK8256-1000 | Akron Biotech | 1mg | Ask for price |
 rHu Leptin |
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| AK8265-0200 | Akron Biotech | 200µg | Ask for price |
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rHu Leptin |
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| AK8265-1000 | Akron Biotech | 1mg | Ask for price |
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rHu Leptin |
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| AK8265-5000 | Akron Biotech | 5mg | Ask for price |
 rHu RANTES |
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| AK8275-0005 | Akron Biotech | 5µg | Ask for price |
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rHu RANTES |
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| AK8275-0020 | Akron Biotech | 20µg | Ask for price |
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rHu RANTES |
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| AK8275-0100 | Akron Biotech | 100µg | Ask for price |
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rHu RANTES |
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| AK8275-1000 | Akron Biotech | 1mg | Ask for price |
 rHu BDNF |
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| AK8286-0002 | Akron Biotech | 2µg | Ask for price |
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rHu BDNF |
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| AK8286-0010 | Akron Biotech | 10µg | Ask for price |
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rHu BDNF |
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| AK8286-0100 | Akron Biotech | 100µg | Ask for price |
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rHu BDNF |
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| AK8286-1000 | Akron Biotech | 1mg | Ask for price |
 rHu CNTF |
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| AK8302-0005 | Akron Biotech | 5µg | Ask for price |
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rHu CNTF |
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| AK8302-0020 | Akron Biotech | 20µg | Ask for price |
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rHu CNTF |
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| AK8302-0100 | Akron Biotech | 100µg | Ask for price |
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rHu CNTF |
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| AK8302-1000 | Akron Biotech | 1mg | Ask for price |
 rHu EGF |
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| AK8327-0100 | Akron Biotech | 100µg | Ask for price |
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rHu EGF |
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| AK8327-0500 | Akron Biotech | 500µg | Ask for price |
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rHu EGF |
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| AK8327-1000 | Akron Biotech | 1mg | Ask for price |
 rHu GDNF |
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| AK8335-0002 | Akron Biotech | 2µg | Ask for price |
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rHu GDNF |
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| AK8335-0010 | Akron Biotech | 10µg | Ask for price |
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rHu GDNF |
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| AK8335-0100 | Akron Biotech | 100µg | Ask for price |
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rHu GDNF |
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| AK8335-1000 | Akron Biotech | 1mg | Ask for price |
 rHu OSM |
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| AK8531-0002 | Akron Biotech | 2µg | Ask for price |
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rHu OSM |
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| AK8531-0010 | Akron Biotech | 10µg | Ask for price |
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rHu OSM |
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| AK8531-0100 | Akron Biotech | 100µg | Ask for price |
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rHu OSM |
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| AK8531-1000 | Akron Biotech | 1mg | Ask for price |
 rHu BAFF |
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| AK8540-0005 | Akron Biotech | 5µg | Ask for price |
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rHu BAFF |
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| AK8540-0020 | Akron Biotech | 20µg | Ask for price |
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rHu BAFF |
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| AK8540-0100 | Akron Biotech | 100µg | Ask for price |
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rHu BAFF |
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| AK8540-1000 | Akron Biotech | 1mg | Ask for price |
 rHu Artemin |
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| AK9076-0005 | Akron Biotech | 5µg | Ask for price |
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rHu Artemin |
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| AK9076-0020 | Akron Biotech | 20µg | Ask for price |
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rHu Artemin |
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| AK9076-0100 | Akron Biotech | 100µg | Ask for price |
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rHu Artemin |
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| AK9076-1000 | Akron Biotech | 1mg | Ask for price |
 rHu Omentin |
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| AK9083-0002 | Akron Biotech | 2µg | Ask for price |
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rHu Omentin |
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| AK9083-0010 | Akron Biotech | 10µg | Ask for price |
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rHu Omentin |
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| AK9083-0100 | Akron Biotech | 100µg | Ask for price |
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rHu Omentin |
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| AK9083-1000 | Akron Biotech | 1mg | Ask for price |
 rHu Resistin |
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| AK9086-0005 | Akron Biotech | 5µg | Ask for price |
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rHu Resistin |
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| AK9086-0025 | Akron Biotech | 25µg | Ask for price |
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rHu Resistin |
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| AK9086-0100 | Akron Biotech | 100µg | Ask for price |
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rHu Resistin |
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| AK9086-1000 | Akron Biotech | 1mg | Ask for price |
 rHu SHH |
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| AK9088-0005 | Akron Biotech | 5µg | Ask for price |
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rHu SHH |
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| AK9088-0025 | Akron Biotech | 25µg | Ask for price |
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rHu SHH |
|||
| AK9088-0100 | Akron Biotech | 100µg | Ask for price |
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rHu SHH |
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| AK9088-1000 | Akron Biotech | 1mg | Ask for price |
 rHu TPO |
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| AK9089-0002 | Akron Biotech | 5µg | Ask for price |
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rHu TPO |
|||
| AK9089-0010 | Akron Biotech | 20µg | Ask for price |
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rHu TPO |
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| AK9089-0100 | Akron Biotech | 100µg | Ask for price |
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rHu TPO |
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| AK9089-1000 | Akron Biotech | 1mg | Ask for price |
 rHu TRAIL |
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| AK9090-0010 | Akron Biotech | 10µg | Ask for price |
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rHu TRAIL |
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| AK9090-0050 | Akron Biotech | 50µg | Ask for price |
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rHu TRAIL |
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| AK9090-0100 | Akron Biotech | 100µg | Ask for price |
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rHu TRAIL |
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| AK9090-1000 | Akron Biotech | 1mg | Ask for price |
 rHu TSLP |
|||
| AK9091-0002 | Akron Biotech | 2µg | Ask for price |
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rHu TSLP |
|||
| AK9091-0010 | Akron Biotech | 10µg | Ask for price |
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rHu TSLP |
|||
| AK9091-0100 | Akron Biotech | 100µg | Ask for price |