Cell Free DNA content material in tradition medium and mitochondrial DNA copy

pharmacodane

Comparative evaluation of cell-free DNA content material in tradition medium and mitochondrial DNA copy quantity in porcine parthenogenetically activated embryos

We examined the impact of ploidy on mitochondrial DNA (mtDNA) copy quantity in embryos and the quantity of cell-free mitochondrial and nucleic DNA content material (cf-mtDNA and cf-nDNA) in spent tradition medium (SCM).
  • Oocytes collected from the ovaries had been matured, activated, incubated in medium containing cycloheximide (CHX) or CHX and cytochalasin B (CB) for 4.5 h to supply haploid or diploid embryos (H-group and D-group embryos).
  • These embryos had been cultured for 7 days, and the blastocysts and SCM had been examined.
  • The quantity of mtDNA and nDNA was decided by real-time PCR. The speed of growth to the blastocyst stage was increased for the D-group than for the H-group.
  • Furthermore, D-group blastocysts had much less mtDNA in comparison with the H-group blastocysts.
  • After activation, the mitochondrial content material was fixed earlier than the blastocyst stage in D-group embryos, however elevated earlier in H-group embryos.
  • The quantity of cf-mtDNA within the SCM of D-group blastocysts was higher than that of H-group blastocysts. Nevertheless, when the cf-mtDNA within the SCM of two cell-stage embryos (day 2 post-activation) was examined, the quantity of cf-mtDNA was higher within the H-group than within the D-group embryos.
  • When D-group embryos had been cultured for 7 days, a big correlation was noticed between the full cell variety of blastocysts and cf-nDNA content material within the SCM.
  • Therefore, though cautious consideration is required relating to the time level for evaluating mtDNA content material within the embryos and SCM, this research demonstrates that mtDNA within the embryos and SCM was affected by the ploidy of the embryos.

Quick compressive lens-free tomography for 3D organic cell tradition imaging

We current a compressive lens-free method that performs tomographic imaging throughout a cubic millimeter-scale quantity from extremely sparse knowledge.

In contrast with present lens-free 3D microscopy methods, our technique requires an order of magnitude fewer multi-angle illuminations for tomographic reconstruction, resulting in a compact, cost-effective and scanning-free setup with a lowered knowledge acquisition time to allow high-throughput 3D imaging of dynamic organic processes. We apply a quick proximal gradient algorithm with composite regularization to deal with the ill-posed tomographic inverse downside.

Utilizing simulated knowledge, we present that the proposed technique can obtain a reconstruction pace ∼10× sooner than the state-of-the-art inverse downside strategy in 3D lens-free microscopy.

We experimentally validate the effectiveness of our technique by imaging a decision check chart and polystyrene beads, demonstrating its functionality to resolve micron-size options in each lateral and axial instructions.

Moreover, tomographic reconstruction outcomes of neuronspheres and intestinal organoids reveal the potential of this 3D imaging method for high-resolution and high-throughput organic purposes.

pharmacodane

pharmacodane

rHu MIP-1-beta

AK8264-0002 2µg Ask for price

rHu MIP-1-beta

AK8264-0010 10µg Ask for price

rHu MIP-1-beta

AK8264-0100 100µg Ask for price

rHu MIP-1-beta

AK8264-1000 1mg Ask for price

rHu MIP-1-alpha

AK8250-0005 5µg Ask for price

rHu MIP-1-alpha

AK8250-0020 20µg Ask for price

rHu MIP-1-alpha

AK8250-0100 100µg Ask for price

rHu MIP-1-alpha

AK8250-1000 1mg Ask for price

rHu MIP-3-alpha

AK8271-0005 5µg Ask for price

rHu MIP-3-alpha

AK8271-0020 20µg Ask for price

rHu MIP-3-alpha

AK8271-0100 100µg Ask for price

rHu MIP-3-alpha

AK8271-1000 1mg Ask for price

rHu IL-4

AK8329-0005 5µg Ask for price

rHu IL-4

AK8329-0020 20µg Ask for price

rHu IL-4

AK8329-0100 100µg Ask for price

rHu IL-4

AK8329-1000 1mg Ask for price

rHu MCP-4

AK8332-0005 5µg Ask for price

rHu MCP-4

AK8332-0020 20µg Ask for price

rHu MCP-4

AK8332-0100 100µg Ask for price

rHu MCP-4

AK8332-1000 1mg Ask for price

rHu FGF-4

AK9698-0005 5µg Ask for price

rHu FGF-4

AK9698-0025 25µg Ask for price

rHu FGF-4

AK9698-0100 100µg Ask for price

rHu FGF-4

AK9698-1000 1mg Ask for price

rHu IL-4 (cGMP)

AK9839-0000 1mg Ask for price

rHu BMP-4 (CHO)

AK9996-0005 5µg Ask for price

rHu BMP-4 (CHO)

AK9996-0020 20µg Ask for price

rHu BMP-4 (CHO)

AK9996-0100 100µg Ask for price

rHu BMP-4 (CHO)

AK9996-1000 1mg Ask for price

MIP-4

GWB-915E16 0.01 mg Ask for price

MIP 4

chm-322 2µg
EUR 60
Description: Recombinant Human Macrophage Inflammatory protein-4 (CCL18)

rHu MIF

AK8255-0005 5µg Ask for price

rHu MIF

AK8255-0025 25µg Ask for price

rHu MIF

AK8255-0100 100µg Ask for price

rHu MIF

AK8255-1000 1mg Ask for price

rHu EGF

AK8327-0100 100µg Ask for price

rHu EGF

AK8327-0500 500µg Ask for price

rHu EGF

AK8327-1000 1mg Ask for price

rHu OSM

AK8531-0002 2µg Ask for price

rHu OSM

AK8531-0010 10µg Ask for price

rHu OSM

AK8531-0100 100µg Ask for price

rHu OSM

AK8531-1000 1mg Ask for price

rHu SHH

AK9088-0005 5µg Ask for price

rHu SHH

AK9088-0025 25µg Ask for price

rHu SHH

AK9088-0100 100µg Ask for price

rHu SHH

AK9088-1000 1mg Ask for price

rHu TPO

AK9089-0002 5µg Ask for price

rHu TPO

AK9089-0010 20µg Ask for price

rHu TPO

AK9089-0100 100µg Ask for price

rHu TPO

AK9089-1000 1mg Ask for price

rHu PTN

AK9912-0005 5µg Ask for price

rHu PTN

AK9912-0020 20µg Ask for price

rHu PTN

AK9912-0100 100µg Ask for price

rHu PTN

AK9912-1000 1mg Ask for price

rHu LIF

AK9977-0005 5µg Ask for price

rHu LIF

AK9977-0025 25µg Ask for price

rHu LIF

AK9977-0100 100µg Ask for price

rHu LIF

AK9977-1000 1mg Ask for price

rHu MIG

AK9979-0005 5µg Ask for price

rHu MIG

AK9979-0020 20µg Ask for price

rHu MIG

AK9979-0100 100µg Ask for price

rHu MIG

AK9979-1000 1mg Ask for price

rHu aFGF

AK8230-0010 10µg Ask for price

rHu aFGF

AK8230-0050 50µg Ask for price

rHu aFGF

AK8230-0100 100µg Ask for price

rHu aFGF

AK8230-1000 1mg Ask for price

rHu MCSF

AK8238-0002 2µg Ask for price

rHu MCSF

AK8238-0010 10µg Ask for price

rHu MCSF

AK8238-0100 100µg Ask for price

rHu MCSF

AK8238-1000 1mg Ask for price

rHu CTGF

AK8240-0005 5µg Ask for price

rHu CTGF

AK8240-0020 20µg Ask for price

rHu CTGF

AK8240-0100 100µg Ask for price

rHu CTGF

AK8240-1000 1mg Ask for price

rHu BDNF

AK8286-0002 2µg Ask for price

rHu BDNF

AK8286-0010 10µg Ask for price

rHu BDNF

AK8286-0100 100µg Ask for price

Adipose Tissue from Lean and Overweight Mice Induces a Mesenchymal to Epithelial Transition-Like Impact in Triple Unfavourable Breast Cancers Cells Grown in 3-Dimensional Tradition

Breast most cancers is the second main reason behind cancer-related mortality amongst ladies globally with weight problems being one threat issue. Overweight breast most cancers sufferers have a minimum of a 30% elevated threat of loss of life from breast most cancers in comparison with non-obese breast most cancers sufferers as a result of they current with bigger tumors and customarily have elevated charges of metastasis.
Furthermore, overweight breast most cancers sufferers reply extra poorly to therapy in comparison with non-obese sufferers, significantly pre-menopausal ladies identified with triple detrimental breast most cancers (TNBC).
To assist perceive the molecular mechanisms underlying the elevated metastasis related to weight problems, we beforehand established a three-dimensional tradition system that allows the co-culture of adipocytes and TNBC cells in a way that mimics an in vivo milieu.
Utilizing this technique, we display that white adipose tissue from each lean and overweight mice can induce a partial mesenchymal-to-epithelial transition (MET).
Triple detrimental breast most cancers cells undertake an epithelial morphology and have an elevated expression of some epithelial markers, however they keep the expression of mesenchymal markers, furnishing the breast most cancers cells with hybrid properties which are related to extra aggressive tumors.
Thus, these knowledge counsel that adipose tissue has the potential to promote secondary tumor formation in lean and overweight ladies. Additional work is required to find out if focusing on the partial MET induced by adipose tissue may scale back metastasis.

Procalcitonin

hor-304 2µg
EUR 60
Description: Recombinant Human Procalcitonin

Recombinant Human Procalcitonin His Tag, Procalcitonin Human, His

MBS142688-0002mg 0.002mg
EUR 240

Recombinant Human Procalcitonin His Tag, Procalcitonin Human, His

MBS142688-001mg 0.01mg
EUR 310

Recombinant Human Procalcitonin His Tag, Procalcitonin Human, His

MBS142688-1mg 1mg
EUR 5040

Recombinant Human Procalcitonin His Tag, Procalcitonin Human, His

MBS142688-5x1mg 5x1mg
EUR 22345

Procalcitonin (PE)

MBS6129538-01mL 0.1(mL
EUR 1345

Procalcitonin (PE)

MBS6129538-5x01mL 5x0.1mL
EUR 5900

Procalcitonin (PE)

MBS6129539-01mL 0.1(mL
EUR 1170

Procalcitonin (PE)

MBS6129539-5x01mL 5x0.1mL
EUR 5115

Procalcitonin (AP)

MBS6126442-01mL 0.1(mL
EUR 1345

Procalcitonin (AP)

MBS6126442-5x01mL 5x0.1mL
EUR 5900

Procalcitonin (AP)

MBS6126443-01mL 0.1(mL
EUR 1170

Procalcitonin (AP)

MBS6126443-5x01mL 5x0.1mL
EUR 5115

Procalcitonin (PCT)

E35P1103 100ug
EUR 295

Procalcitonin His

hor-295 2µg
EUR 60
Description: Recombinant Human Procalcitonin His Tag

Procalcitonin (HRP)

MBS6128918-01mL 0.1(mL
EUR 1345

Procalcitonin (HRP)

MBS6128918-5x01mL 5x0.1mL
EUR 5900

Procalcitonin (HRP)

MBS6128919-01mL 0.1(mL
EUR 1170

Procalcitonin (HRP)

MBS6128919-5x01mL 5x0.1mL
EUR 5115

Procalcitonin (APC)

MBS6127062-01mL 0.1(mL
EUR 1345

Procalcitonin (APC)

MBS6127062-5x01mL 5x0.1mL
EUR 5900

Procalcitonin (APC)

MBS6127063-01mL 0.1(mL
EUR 1170

Procalcitonin (APC)

MBS6127063-5x01mL 5x0.1mL
EUR 5115

Procalcitonin (PCT)

MBS8575447-01mg 0.1mg
EUR 370

Procalcitonin (PCT)

MBS8575447-5x01mg 5x0.1mg
EUR 1520

Procalcitonin (PCT)

MBS343114-001mg 0.01mg
EUR 245

Procalcitonin (PCT)

MBS343114-01mg 0.1mg
EUR 585

Procalcitonin (PCT)

MBS343114-1mg 1mg
EUR 3300

Procalcitonin (PCT)

MBS343114-5x1mg 5x1mg
EUR 14680

Procalcitonin, PCT

MBS355754-001mg 0.01mg
EUR 235

Procalcitonin, PCT

MBS355754-5x001mg 5x0.01mg
EUR 755

Procalcitonin (PCT)

MBS340365-10mg 10mg
EUR 800

Procalcitonin (PCT)

MBS340365-5x10mg 5x10mg
EUR 3435

Procalcitonin (PCT)

MBS623342-5mg 5mg
EUR 970

Procalcitonin (PCT)

MBS623342-5x5mg 5x5mg
EUR 4210

Procalcitonin (FITC)

MBS6128297-01mL 0.1(mL
EUR 1345

Procalcitonin (FITC)

MBS6128297-5x01mL 5x0.1mL
EUR 5900

Procalcitonin (FITC)

MBS6128298-01mL 0.1(mL
EUR 1170

Procalcitonin (FITC)

MBS6128298-5x01mL 5x0.1mL
EUR 5115

Procalcitonin (Biotin)

MBS6127682-01mL 0.1(mL
EUR 1345

Procalcitonin (Biotin)

MBS6127682-5x01mL 5x0.1mL
EUR 5900

Procalcitonin (Biotin)

MBS6127683-01mL 0.1(mL
EUR 1170

Procalcitonin (Biotin)

MBS6127683-5x01mL 5x0.1mL
EUR 5115

Procalcitonin Protein

abx061017-100ug 100 ug
EUR 2298

Procalcitonin protein

30-1379 500 ug
EUR 338
Description: Recombinant Procalcitonin protein

Procalcitonin protein

30-1908 100 ug
EUR 680
Description: Purified Recombinant Procalcitonin protein

Procalcitonin protein

30R-2425 10 ug
EUR 287
Description: Purified recombinant Human Procalcitonin protein

Procalcitonin Protein

20-abx262327
  • Ask for price
  • Ask for price
  • Ask for price
  • 10 ug
  • 1 mg
  • 2 µg

Procalcitonin Protein

20-abx262966
  • Ask for price
  • Ask for price
  • Ask for price
  • 10 ug
  • 1 mg
  • 2 µg

Procalcitonin Protein

20-abx262989
  • Ask for price
  • Ask for price
  • Ask for price
  • 10 ug
  • 1 mg
  • 2 µg

Procalcitonin protein

MBS5308888-05mg 0.5mg
EUR 500

Procalcitonin protein

MBS5308888-5x05mg 5x0.5mg
EUR 2020

MAb to Procalcitonin

MBS313024-1mg 1mg
EUR 865

MAb to Procalcitonin

MBS313024-5x1mg 5x1mg
EUR 3725

MAb to Procalcitonin

MBS313117-1mg 1mg
EUR 1545

MAb to Procalcitonin

MBS313117-5x1mg 5x1mg
EUR 6770

MAb to Procalcitonin

MBS313256-1mg 1mg
EUR 900

MAb to Procalcitonin

MBS313256-5x1mg 5x1mg
EUR 3875

MAb to Procalcitonin

MBS313351-1mg 1mg
EUR 900

MAb to Procalcitonin

MBS313351-5x1mg 5x1mg
EUR 3875

MAb to Procalcitonin

MBS310731-1mg 1mg
EUR 865

MAb to Procalcitonin

MBS310731-5x1mg 5x1mg
EUR 3725

MAb to Procalcitonin

MBS310732-1mg 1mg
EUR 865

MAb to Procalcitonin

MBS310732-5x1mg 5x1mg
EUR 3725

MAb to Procalcitonin

MBS310747-1mg 1mg
EUR 865

MAb to Procalcitonin

MBS310747-5x1mg 5x1mg
EUR 3725

MAb to Procalcitonin

MBS312689-1mg 1mg
EUR 865

MAb to Procalcitonin

MBS312689-5x1mg 5x1mg
EUR 3725

Procalcitonin Antibody

20-abx121310
  • Ask for price
  • Ask for price
  • Ask for price
  • 100 ul
  • 200 ul
  • 30 ul

Procalcitonin Antibody

abx023816-1mg 1 mg
EUR 1053.6

Procalcitonin Antibody

abx023817-1mg 1 mg
EUR 1053.6

Procalcitonin Antibody

6640-100 each
EUR 326.4

Procalcitonin Antibody

6640-30T each
EUR 175.2

Procalcitonin antibody

10-7941 500 ug
EUR 262
Description: Mouse monoclonal Procalcitonin antibody

Procalcitonin antibody

10-7942 500 ug
EUR 262
Description: Mouse monoclonal Procalcitonin antibody

Procalcitonin antibody

10-7943 500 ug
EUR 262
Description: Mouse monoclonal Procalcitonin antibody

Procalcitonin antibody

10-7944 500 ug
EUR 262
Description: Mouse monoclonal Procalcitonin antibody

Procalcitonin Antibody

E38PA3809 100ul
EUR 225
Description: Available in various conjugation types.

Procalcitonin Antibody

GWB-26B91D 1 mg Ask for price

Procalcitonin Antibody

GWB-307E00 0.1 mg Ask for price

Procalcitonin Antibody

GWB-T00237 1 mg Ask for price

Procalcitonin Antibody

GWB-T00300 1 mg Ask for price

Procalcitonin Antibody

GWB-T00301 1 mg Ask for price

Procalcitonin Antibody

GWB-6E5D17 0.1 mg Ask for price

Procalcitonin Antibody

GWB-720CDE 1 mg Ask for price

Procalcitonin Antibody

GWB-74A5CF 0.1 mg Ask for price

Procalcitonin Antibody

GWB-B773E4 0.1 mg Ask for price

Procalcitonin Antibody

GWB-EFAC6B 1 mg Ask for price

Procalcitonin Antibody

GWB-C9812F 0.1 mg Ask for price

Procalcitonin Antibody

MBS8582169-01mL 0.1mL
EUR 305

Procalcitonin Antibody

MBS8582169-01mLAF405L 0.1mL(AF405L)
EUR 465

Procalcitonin Antibody

MBS8582169-01mLAF405S 0.1mL(AF405S)
EUR 465

Procalcitonin Antibody

MBS8582169-01mLAF610 0.1mL(AF610)
EUR 465

Procalcitonin Antibody

MBS8582169-01mLAF635 0.1mL(AF635)
EUR 465

Human Procalcitonin / PCT

MBS355727-5x96Tests 5x96Tests
EUR 1935

Human Procalcitonin / PCT

MBS355727-96Tests 96Tests
EUR 435

Control FD Procalcitonin

K071M-6 6 x 2 mL
EUR 147
Description: Bi-Level

Dog Procalcitonin Protein

20-abx262429
  • Ask for price
  • Ask for price
  • Ask for price
  • 10 ug
  • 1 mg
  • 2 µg

Skriv et svar

Din e-mailadresse vil ikke blive publiceret.