Cell Free DNA content material in tradition medium and mitochondrial DNA copy

      Ingen kommentarer til Cell Free DNA content material in tradition medium and mitochondrial DNA copy
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Comparative evaluation of cell-free DNA content material in tradition medium and mitochondrial DNA copy quantity in porcine parthenogenetically activated embryos

We examined the impact of ploidy on mitochondrial DNA (mtDNA) copy quantity in embryos and the quantity of cell-free mitochondrial and nucleic DNA content material (cf-mtDNA and cf-nDNA) in spent tradition medium (SCM).
  • Oocytes collected from the ovaries had been matured, activated, incubated in medium containing cycloheximide (CHX) or CHX and cytochalasin B (CB) for 4.5 h to supply haploid or diploid embryos (H-group and D-group embryos).
  • These embryos had been cultured for 7 days, and the blastocysts and SCM had been examined.
  • The quantity of mtDNA and nDNA was decided by real-time PCR. The speed of growth to the blastocyst stage was increased for the D-group than for the H-group.
  • Furthermore, D-group blastocysts had much less mtDNA in comparison with the H-group blastocysts.
  • After activation, the mitochondrial content material was fixed earlier than the blastocyst stage in D-group embryos, however elevated earlier in H-group embryos.
  • The quantity of cf-mtDNA within the SCM of D-group blastocysts was higher than that of H-group blastocysts. Nevertheless, when the cf-mtDNA within the SCM of two cell-stage embryos (day 2 post-activation) was examined, the quantity of cf-mtDNA was higher within the H-group than within the D-group embryos.
  • When D-group embryos had been cultured for 7 days, a big correlation was noticed between the full cell variety of blastocysts and cf-nDNA content material within the SCM.
  • Therefore, though cautious consideration is required relating to the time level for evaluating mtDNA content material within the embryos and SCM, this research demonstrates that mtDNA within the embryos and SCM was affected by the ploidy of the embryos.

Quick compressive lens-free tomography for 3D organic cell tradition imaging

We current a compressive lens-free method that performs tomographic imaging throughout a cubic millimeter-scale quantity from extremely sparse knowledge.

In contrast with present lens-free 3D microscopy methods, our technique requires an order of magnitude fewer multi-angle illuminations for tomographic reconstruction, resulting in a compact, cost-effective and scanning-free setup with a lowered knowledge acquisition time to allow high-throughput 3D imaging of dynamic organic processes. We apply a quick proximal gradient algorithm with composite regularization to deal with the ill-posed tomographic inverse downside.

Utilizing simulated knowledge, we present that the proposed technique can obtain a reconstruction pace ∼10× sooner than the state-of-the-art inverse downside strategy in 3D lens-free microscopy.

We experimentally validate the effectiveness of our technique by imaging a decision check chart and polystyrene beads, demonstrating its functionality to resolve micron-size options in each lateral and axial instructions.

Moreover, tomographic reconstruction outcomes of neuronspheres and intestinal organoids reveal the potential of this 3D imaging method for high-resolution and high-throughput organic purposes.

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rHu MIP-1-alpha
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rHu MIP-1-beta
AK8264-0002 2µg Ask for price

rHu MIP-1-beta
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rHu MIP-3-alpha
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rHu IL-4
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rHu IL-4
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rHu IL-4
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rHu IL-4
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rHu FGF-4
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rHu IL-4 (cGMP)
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rHu BMP-4 (CHO)
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MIP-4 Recombinant Protein
40-385-0002mg 0.002 mg
EUR 311.1
Description: MIP-4 is a CC chemokine that is expressed in lymph nodes, lungs, placenta and bone marrow. MIP-4's primary receptor is unknown. MIP-4 chemoattracts lymphocytes, and has been shown to exert activity on both CD4+ and CD8+ T cells. Recombinant human MIP-4 is a 7.8 kDa protein containing 69 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

MIP-4 Recombinant Protein
40-385-001mg 0.01 mg
EUR 437.1
Description: MIP-4 is a CC chemokine that is expressed in lymph nodes, lungs, placenta and bone marrow. MIP-4's primary receptor is unknown. MIP-4 chemoattracts lymphocytes, and has been shown to exert activity on both CD4+ and CD8+ T cells. Recombinant human MIP-4 is a 7.8 kDa protein containing 69 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

MIP-4/CCL18, Human
HY-P7265 50ug
EUR 639.6

Recombinant Human Interleukin-4 (rHu IL-4)
AK9839-0010 10ug
EUR 780

Recombinant Human Interleukin-4 (rHu IL-4)
AK9839-0030 30ug
EUR 1356

Recombinant Human Interleukin-4 (rHu IL-4)
AK9839-0100 100ug
EUR 2040

Sheep MIP-4 ELISA Kit
ESM0021 96Tests
EUR 625.2

Human MIP-4 ELISA Kit
EHM0021 96Tests
EUR 625.2

Porcine MIP-4 ELISA Kit
EPM0021 96Tests
EUR 625.2

Monkey MIP-4 ELISA Kit
EMKM0021 96Tests
EUR 625.2

Mouse MIP-4 ELISA Kit
EMM0021 96Tests
EUR 625.2

Rat MIP-4 ELISA Kit
ERM0021 96Tests
EUR 625.2

Rabbit MIP-4 ELISA Kit
ERTM0021 96Tests
EUR 625.2

Goat MIP-4 ELISA Kit
EGTM0021 96Tests
EUR 625.2

Bovine MIP-4 ELISA Kit
EBM0021 96Tests
EUR 625.2

Anserini MIP-4 ELISA Kit
EAM0021 96Tests
EUR 625.2

Chicken MIP-4 ELISA Kit
ECKM0021 96Tests
EUR 625.2

Canine MIP-4 ELISA Kit
ECM0021 96Tests
EUR 625.2

MIP-4, PARC, CCL18, human
RC315-29 2ug
EUR 125.26

MIP-4 (PARC), human recombinant
4257-10 each
EUR 405.6

MIP-4 (PARC), human recombinant
4257-1000 each
EUR 7100.4

Guinea Pig MIP-4 ELISA Kit
EGM0021 96Tests
EUR 625.2

Recombinant Human MIP-4 (CCL18) Protein
PROTP55774-2 10ug
EUR 380.4
Description: MIP-4 is a CC chemokine that is expressed in lymph nodes, lungs, placenta and bone marrow. MIP-4's primary receptor is unknown. MIP-4 chemoattracts lymphocytes, and has been shown to exert activity on both CD4+ and CD8+ T cells. Recombinant human MIP-4 is a 7.8 kDa protein containing 68 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

IL-4 Interleukin 4 Human Recombinant Protein, Yeast
PROTP05112-4 Regular: 10ug
EUR 380.4
Description: Interleukin-4 Human Recombinant produced in yeast is a single, glycosylated polypeptide chain containing 129 amino acids.;The IL-4 is purified by proprietary chromatographic techniques.

Individual Reaction Mix 4
G065-4 200 reactions
EUR 200.4

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Adipose Tissue from Lean and Overweight Mice Induces a Mesenchymal to Epithelial Transition-Like Impact in Triple Unfavourable Breast Cancers Cells Grown in 3-Dimensional Tradition

Breast most cancers is the second main reason behind cancer-related mortality amongst ladies globally with weight problems being one threat issue. Overweight breast most cancers sufferers have a minimum of a 30% elevated threat of loss of life from breast most cancers in comparison with non-obese breast most cancers sufferers as a result of they current with bigger tumors and customarily have elevated charges of metastasis.
Furthermore, overweight breast most cancers sufferers reply extra poorly to therapy in comparison with non-obese sufferers, significantly pre-menopausal ladies identified with triple detrimental breast most cancers (TNBC).
To assist perceive the molecular mechanisms underlying the elevated metastasis related to weight problems, we beforehand established a three-dimensional tradition system that allows the co-culture of adipocytes and TNBC cells in a way that mimics an in vivo milieu.
Utilizing this technique, we display that white adipose tissue from each lean and overweight mice can induce a partial mesenchymal-to-epithelial transition (MET).
Triple detrimental breast most cancers cells undertake an epithelial morphology and have an elevated expression of some epithelial markers, however they keep the expression of mesenchymal markers, furnishing the breast most cancers cells with hybrid properties which are related to extra aggressive tumors.
Thus, these knowledge counsel that adipose tissue has the potential to promote secondary tumor formation in lean and overweight ladies. Additional work is required to find out if focusing on the partial MET induced by adipose tissue may scale back metastasis.

Procalcitonin protein
30-1908 100 ug
EUR 1117.2
Description: Purified Recombinant Procalcitonin protein

Procalcitonin antibody
10-7941 500 ug
EUR 405.6
Description: Mouse monoclonal Procalcitonin antibody

Procalcitonin antibody
10-7942 500 ug
EUR 405.6
Description: Mouse monoclonal Procalcitonin antibody

Procalcitonin antibody
10-7943 500 ug
EUR 405.6
Description: Mouse monoclonal Procalcitonin antibody

Procalcitonin antibody
10-7944 500 ug
EUR 405.6
Description: Mouse monoclonal Procalcitonin antibody

Procalcitonin Antibody
20-abx121310
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

Procalcitonin Antibody
abx023816-1mg 1 mg
EUR 1053.6

Procalcitonin Antibody
abx023817-1mg 1 mg
EUR 1053.6

Procalcitonin Protein
abx061017-100ug 100 ug
EUR 2298

Procalcitonin Protein
20-abx262327
  • EUR 393.60
  • EUR 7191.60
  • EUR 276.00
  • 10 ug
  • 1 mg
  • 2 µg

Procalcitonin Protein
20-abx262966
  • EUR 393.60
  • EUR 7676.40
  • EUR 276.00
  • 10 ug
  • 1 mg
  • 2 µg

Procalcitonin Protein
20-abx262989
  • EUR 393.60
  • EUR 8010.00
  • EUR 276.00
  • 10 ug
  • 1 mg
  • 2 µg

Procalcitonin Antibody
6640-100 each
EUR 326.4

Procalcitonin Antibody
6640-30T each
EUR 175.2

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rHu BAFF
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rHu Artemin
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