Comparative analysis of cell-free DNA content in culture

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Comparative analysis of cell-free DNA content in culture medium and mitochondrial DNA copy number in porcine parthenogenetically activated embryos

We examined the effect of ploidy on mitochondrial DNA (mtDNA) copy number in embryos and the amount of cell-free mitochondrial and nucleic DNA content (cf-mtDNA and cf-nDNA) in spent culture medium (SCM). Oocytes collected from the ovaries were matured, activated, incubated in medium containing cycloheximide (CHX) or CHX and cytochalasin B (CB) for 4.5 h to produce haploid or diploid embryos (H-group and D-group embryos).
These embryos were cultured for 7 days, and the blastocysts and SCM were examined. The amount of mtDNA and nDNA was determined by real-time PCR. The rate of development to the blastocyst stage was higher for the D-group than for the H-group. Moreover, D-group blastocysts had less mtDNA compared to the H-group blastocysts. After activation, the mitochondrial content was constant before the blastocyst stage in D-group embryos, but increased earlier in H-group embryos. The amount of cf-mtDNA in the SCM of D-group blastocysts was greater than that of H-group blastocysts.
However, when the cf-mtDNA in the SCM of 2 cell-stage embryos (day 2 post-activation) was examined, the amount of cf-mtDNA was greater in the H-group than in the D-group embryos. When D-group embryos were cultured for 7 days, a significant correlation was observed between the total cell number of blastocysts and cf-nDNA content in the SCM. Hence, although careful consideration is needed regarding the time point for evaluating mtDNA content in the embryos and SCM, this study demonstrates that mtDNA in the embryos and SCM was affected by the ploidy of the embryos.
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Human Oncostatin M (OSM) ELISA Kit
RDR-OSM-Hu-48Tests 48 Tests
EUR 500

Human Oncostatin M (OSM) ELISA Kit
RDR-OSM-Hu-96Tests 96 Tests
EUR 692

Mouse Oncostatin M (OSM) ELISA Kit
RDR-OSM-Mu-48Tests 48 Tests
EUR 511

Mouse Oncostatin M (OSM) ELISA Kit
RDR-OSM-Mu-96Tests 96 Tests
EUR 709

Rat Oncostatin M (OSM) ELISA Kit
RDR-OSM-Ra-48Tests 48 Tests
EUR 534

Rat Oncostatin M (OSM) ELISA Kit
RDR-OSM-Ra-96Tests 96 Tests
EUR 742

Bovine Oncostatin M (OSM) ELISA Kit
RD-OSM-b-48Tests 48 Tests
EUR 555

Bovine Oncostatin M (OSM) ELISA Kit
RD-OSM-b-96Tests 96 Tests
EUR 771

Human Oncostatin M (OSM) ELISA Kit
RD-OSM-Hu-48Tests 48 Tests
EUR 478

Human Oncostatin M (OSM) ELISA Kit
RD-OSM-Hu-96Tests 96 Tests
EUR 662

Mouse Oncostatin M (OSM) ELISA Kit
RD-OSM-Mu-48Tests 48 Tests
EUR 489

Mouse Oncostatin M (OSM) ELISA Kit
RD-OSM-Mu-96Tests 96 Tests
EUR 677

Rat Oncostatin M (OSM) ELISA Kit
RD-OSM-Ra-48Tests 48 Tests
EUR 511

Rat Oncostatin M (OSM) ELISA Kit
RD-OSM-Ra-96Tests 96 Tests
EUR 709

Bovine Oncostatin M (OSM) ELISA Kit
DLR-OSM-b-48T 48T
EUR 547
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Oncostatin M (OSM) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Bovine Oncostatin M (OSM) ELISA Kit
DLR-OSM-b-96T 96T
EUR 715
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Oncostatin M (OSM) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Oncostatin M (OSM) ELISA Kit
DLR-OSM-Hu-48T 48T
EUR 479
Description: A sandwich quantitative ELISA assay kit for detection of Human Oncostatin M (OSM) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Oncostatin M (OSM) ELISA Kit
DLR-OSM-Hu-96T 96T
EUR 621
Description: A sandwich quantitative ELISA assay kit for detection of Human Oncostatin M (OSM) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Mouse Oncostatin M (OSM) ELISA Kit
DLR-OSM-Mu-48T 48T
EUR 489
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Oncostatin M (OSM) in samples from serum, plasma or other biological fluids.

Mouse Oncostatin M (OSM) ELISA Kit
DLR-OSM-Mu-96T 96T
EUR 635
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Oncostatin M (OSM) in samples from serum, plasma or other biological fluids.

Rat Oncostatin M (OSM) ELISA Kit
DLR-OSM-Ra-48T 48T
EUR 508
Description: A sandwich quantitative ELISA assay kit for detection of Rat Oncostatin M (OSM) in samples from serum, plasma, tissue homogenates or other biological fluids.

Rat Oncostatin M (OSM) ELISA Kit
DLR-OSM-Ra-96T 96T
EUR 661
Description: A sandwich quantitative ELISA assay kit for detection of Rat Oncostatin M (OSM) in samples from serum, plasma, tissue homogenates or other biological fluids.

OSM, Mouse
HY-P7275 50ug
EUR 762

OSM, Rat
HY-P7105 100ug
EUR 1256

OSM siRNA
20-abx927424
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

OSM siRNA
20-abx927425
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

OSM Antibody
37783-100ul 100ul
EUR 252

OSM siRNA
20-abx903778
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

OSM Antibody
1-CSB-PA09694A0Rb
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against OSM. Recognizes OSM from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200

OSM Antibody
1-CSB-PA244255
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against OSM. Recognizes OSM from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:50-1:200

Osm Antibody
1-CSB-PA723970LA01RA
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against Osm. Recognizes Osm from Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IF; Recommended dilution: IF:1:50-1:200

OSM Antibody
1-CSB-PA934414
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against OSM. Recognizes OSM from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:2000, IHC:1:25-1:100

OSM antibody
10R-5127 100 ul
EUR 726
Description: Mouse monoclonal OSM antibody

OSM protein
30R-3271 50 ug
EUR 257
Description: Purified recombinant OSM protein

rHu Insulin
AK8211-0025 25mg Ask for price

rHu Insulin
AK8211-0250 250mg Ask for price

rHu Insulin
AK8211-1000 1g Ask for price

rHu aFGF
AK8230-0010 10µg Ask for price

rHu aFGF
AK8230-0050 50µg Ask for price

rHu aFGF
AK8230-0100 100µg Ask for price

rHu aFGF
AK8230-1000 1mg Ask for price

rHu MCSF
AK8238-0002 2µg Ask for price

rHu MCSF
AK8238-0010 10µg Ask for price

rHu MCSF
AK8238-0100 100µg Ask for price

rHu MCSF
AK8238-1000 1mg Ask for price

rHu CTGF
AK8240-0005 5µg Ask for price

rHu CTGF
AK8240-0020 20µg Ask for price

rHu CTGF
AK8240-0100 100µg Ask for price

rHu CTGF
AK8240-1000 1mg Ask for price

rHu MIF
AK8255-0005 5µg Ask for price

rHu MIF
AK8255-0025 25µg Ask for price

rHu MIF
AK8255-0100 100µg Ask for price

rHu MIF
AK8255-1000 1mg Ask for price

rHu Myostatin
AK8256-0002 2µg Ask for price

rHu Myostatin
AK8256-0010 10µg Ask for price

rHu Myostatin
AK8256-0100 100µg Ask for price

rHu Myostatin
AK8256-1000 1mg Ask for price

rHu Leptin
AK8265-0200 200µg Ask for price

rHu Leptin
AK8265-1000 1mg Ask for price

rHu Leptin
AK8265-5000 5mg Ask for price

rHu RANTES
AK8275-0005 5µg Ask for price

rHu RANTES
AK8275-0020 20µg Ask for price

rHu RANTES
AK8275-0100 100µg Ask for price

rHu RANTES
AK8275-1000 1mg Ask for price

rHu BDNF
AK8286-0002 2µg Ask for price

rHu BDNF
AK8286-0010 10µg Ask for price

rHu BDNF
AK8286-0100 100µg Ask for price

rHu BDNF
AK8286-1000 1mg Ask for price

rHu CNTF
AK8302-0005 5µg Ask for price

rHu CNTF
AK8302-0020 20µg Ask for price

rHu CNTF
AK8302-0100 100µg Ask for price

rHu CNTF
AK8302-1000 1mg Ask for price

rHu EGF
AK8327-0100 100µg Ask for price

rHu EGF
AK8327-0500 500µg Ask for price

rHu EGF
AK8327-1000 1mg Ask for price

rHu GDNF
AK8335-0002 2µg Ask for price

rHu GDNF
AK8335-0010 10µg Ask for price

rHu GDNF
AK8335-0100 100µg Ask for price

rHu GDNF
AK8335-1000 1mg Ask for price

rHu BAFF
AK8540-0005 5µg Ask for price

rHu BAFF
AK8540-0020 20µg Ask for price

rHu BAFF
AK8540-0100 100µg Ask for price

rHu BAFF
AK8540-1000 1mg Ask for price

rHu Artemin
AK9076-0005 5µg Ask for price

rHu Artemin
AK9076-0020 20µg Ask for price

rHu Artemin
AK9076-0100 100µg Ask for price

rHu Artemin
AK9076-1000 1mg Ask for price

rHu Omentin
AK9083-0002 2µg Ask for price

rHu Omentin
AK9083-0010 10µg Ask for price

rHu Omentin
AK9083-0100 100µg Ask for price

rHu Omentin
AK9083-1000 1mg Ask for price

rHu Procalcitonin
AK9084-0002 2µg Ask for price

rHu Procalcitonin
AK9084-0010 10µg Ask for price

rHu Procalcitonin
AK9084-0100 100µg Ask for price

Fast compressive lens-free tomography for 3D biological cell culture imaging

We present a compressive lens-free technique that performs tomographic imaging across a cubic millimeter-scale volume from highly sparse data. Compared with existing lens-free 3D microscopy systems, our method requires an order of magnitude fewer multi-angle illuminations for tomographic reconstruction, leading to a compact, cost-effective and scanning-free setup with a reduced data acquisition time to enable high-throughput 3D imaging of dynamic biological processes.
We apply a fast proximal gradient algorithm with composite regularization to address the ill-posed tomographic inverse problem. Using simulated data, we show that the proposed method can achieve a reconstruction speed ∼10× faster than the state-of-the-art inverse problem approach in 3D lens-free microscopy. We experimentally validate the effectiveness of our method by imaging a resolution test chart and polystyrene beads, demonstrating its capability to resolve micron-size features in both lateral and axial directions.
Furthermore, tomographic reconstruction results of neuronspheres and intestinal organoids reveal the potential of this 3D imaging technique for high-resolution and high-throughput biological applications.

Adipose Tissue from Lean and Obese Mice Induces a Mesenchymal to Epithelial Transition-Like Effect in Triple Negative Breast Cancers Cells Grown in 3-Dimensional Culture

Breast cancer is the second leading cause of cancer-related mortality among women globally with obesity being one risk factor. Obese breast cancer patients have at least a 30% increased risk of death from breast cancer compared to non-obese breast cancer patients because they present with larger tumors and generally have increased rates of metastasis.
Moreover, obese breast cancer patients respond more poorly to treatment compared to non-obese patients, particularly pre-menopausal women diagnosed with triple negative breast cancer (TNBC). To help understand the molecular mechanisms underlying the increased metastasis associated with obesity, we previously established a three-dimensional culture system that permits the co-culture of adipocytes and TNBC cells in a manner that mimics an in vivo milieu.
Using this system, we demonstrate that white adipose tissue from both lean and obese mice can induce a partial mesenchymal-to-epithelial transition (MET). Triple negative breast cancer cells adopt an epithelial morphology and have an increased expression of some epithelial markers, but they maintain the expression of mesenchymal markers, furnishing the breast cancer cells with hybrid properties that are associated with more aggressive tumors.
Thus, these data suggest that adipose tissue has the potential to promote secondary tumor formation in lean and obese women. Further work is needed to determine if targeting the partial MET induced by adipose tissue could reduce metastasis.

1 mm Preparative Comb, RM
2328934 3unit
EUR 253
Description: 1 sampl e wel l and 1 r efer ence wel l

0.75mm Plate Set,RM-075
2393014 2unit
EUR 263
Description: 1 pai r of gl ass pl at es + 1 gask et , for 0.75 mm gel

5 mm Dummy Plate, RM
2393074 10unit
EUR 241
Description: Pl ast i c pl at e for r unni ng 1 Pagel pr ecast gel onl y

2mm Plate Set,RM-22
2393740 3unit
EUR 281
Description: 1 pai r of gl ass pl at es + 1 gask et , for 2 mm gel

7 mm Dummy Plate, RM
2398247 10unit
EUR 241
Description: Pl ast i c pl at e for r unni ng 1 hand-cast gel onl y

1 mm Blank Comb, RM
2399041 4unit
EUR 272

1 mm 6-well Comb, RM
2328375 3unit
EUR 255

1 mm 8-well Comb, RM
2328376 3unit
EUR 255

1 mm 20-well Comb, RM
2328422 3unit
EUR 311

1 mm 10-well Comb, RM
2328948 3unit
EUR 288

A-M310 agarGEL RM, 10/pk
2332200 2unit
EUR 351
Description: pH 3 - 10

A-M38 agarGEL RM, 10/pk
2332210 2unit
EUR 351
Description: pH 3 - 8

A-M58 agarGEL RM, 10/pk
2332220 2unit
EUR 351
Description: pH 5 - 8

A-M510 agarGEL RM, 10/pk
2332230 2unit
EUR 351
Description: pH 5 - 10

1 mm Plate Set, RM-10
2398214 3unit
EUR 281
Description: 1 pai r of gl ass pl at es + 1 gask et , for 1 mm gel

0.75mm Glass plates 2/pk,RM
2398228 2unit
EUR 279
Description: N ot ched pl at e wi t h 0.75mm si de-spacer s

2mm Glass plates 2/pk, RM
2398236 2unit
EUR 249
Description: N ot ched pl at e wi t h 2mm si de-spacer s

1 mm Gaskets 3/pk, RM
2398237 5unit
EUR 257

0.75mm 12-well Combs 2pk,RM
2398271 2unit
EUR 269

Leptin
E21-067 10ug
EUR 343

Leptin
RA21019 50 ug
EUR 435

Leptin
RA30011 50 ug
EUR 605

Leptin
GT15011 100 ug
EUR 526

1mm 14-well Combs 2/pk,RM
2398224 5unit
EUR 272

1mm 18-well Combs 2/pk,RM
2398252 5unit
EUR 272

Leptin, Human
HY-P7232 100ug
EUR 187

Leptin, Rat
HY-P7233 50ug
EUR 153

Leptin Protein
20-abx060295
  • EUR 411.00
  • EUR 1330.00
  • 100 ug
  • 1 mg

Human Leptin
7-01138 200µg Ask for price

Human Leptin
7-01139 1mg Ask for price

Human Leptin
7-01140 5mg Ask for price

Leptin Protein
20-abx260622
  • EUR 3042.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 25 ug
  • 5 ug

Leptin Protein
20-abx262112
  • EUR 328.00
  • EUR 230.00
  • EUR 704.00
  • 1 mg
  • 200 ug
  • 5 mg

Leptin Protein
20-abx262117
  • EUR 328.00
  • EUR 230.00
  • EUR 704.00
  • 1 mg
  • 200 ug
  • 5 mg

Leptin Protein
20-abx262118
  • EUR 328.00
  • EUR 230.00
  • EUR 704.00
  • 1 mg
  • 200 ug
  • 5 mg

Leptin Protein
20-abx262119
  • EUR 328.00
  • EUR 230.00
  • EUR 704.00
  • 1 mg
  • 200 ug
  • 5 mg

Leptin Protein
20-abx263119
  • EUR 328.00
  • EUR 1302.00
  • EUR 230.00
  • 100 ug
  • 1 mg
  • 20 ug

Leptin Protein
20-abx263120
  • EUR 328.00
  • EUR 1302.00
  • EUR 230.00
  • 100 ug
  • 1 mg
  • 20 ug

Leptin Protein
20-abx263121
  • EUR 328.00
  • EUR 1302.00
  • EUR 230.00
  • 100 ug
  • 1 mg
  • 20 ug

Leptin Protein
20-abx263122
  • EUR 328.00
  • EUR 1302.00
  • EUR 230.00
  • 100 ug
  • 1 mg
  • 20 ug

Leptin Protein
20-abx263123
  • EUR 328.00
  • EUR 1302.00
  • EUR 230.00
  • 100 ug
  • 1 mg
  • 20 ug

Leptin Protein
20-abx263124
  • EUR 328.00
  • EUR 1302.00
  • EUR 230.00
  • 100 ug
  • 1 mg
  • 20 ug

Leptin Protein
20-abx263175
  • EUR 328.00
  • EUR 1428.00
  • EUR 230.00
  • 100 ug
  • 1 mg
  • 20 ug

Leptin, human
RC412-17 5mg
EUR 490.8

Leptin (Human)
MO15019 500 ug
EUR 910

Leptin-Human
PR27097 200 ug
EUR 191

Leptin-Mouse
PR27099 200 ug
EUR 191

Leptin-Rat
PR27100 200 ug
EUR 191

Leptin-Pig
PR27137 20 ug
EUR 191

Leptin Receptor
GT15012 100 ug
EUR 526

Leptin Receptor
GT15059 100 ug
EUR 526

rHu Leptin
AK8265-0200 200µg Ask for price

rHu Leptin
AK8265-1000 1mg Ask for price

rHu Leptin
AK8265-5000 5mg Ask for price

Leptin Antibody
5366-100
EUR 316

Leptin Antibody
5366-30T
EUR 146

Leptin Antibody
5367-100
EUR 316

Leptin Antibody
5367-30T
EUR 146

Leptin Antibody
5368-100
EUR 316

Leptin Antibody
5368-30T
EUR 146

Leptin antibody
10-1757 200 ul
EUR 543
Description: Mouse monoclonal Leptin antibody

Leptin antibody
10-1758 200 ul
EUR 543
Description: Mouse monoclonal Leptin antibody

Leptin antibody
10-1759 200 ul
EUR 543
Description: Mouse monoclonal Leptin antibody

Leptin antibody
10-2297 100 ug
EUR 408
Description: Recombinant Fab monoclonal Leptin antibody

Leptin antibody
10-2298 100 ug
EUR 408
Description: Recombinant Fab monoclonal Leptin antibody

Leptin antibody
10-2399 250 ug
EUR 349
Description: Mouse Monoclonal Leptin antibody

Leptin antibody
10-2400 250 ug
EUR 403
Description: Mouse Monoclonal Leptin antibody

Leptin antibody
10-2401 500 ug
EUR 500
Description: Mouse Monoclonal Leptin antibody

Leptin antibody
10-7820 500 ug
EUR 403
Description: Mouse monoclonal Leptin antibody

Leptin antibody
10-7821 500 ug
EUR 403
Description: Mouse monoclonal Leptin antibody

Leptin antibody
10-7822 500 ug
EUR 403
Description: Mouse monoclonal Leptin antibody

Leptin antibody
10R-7769 500 ug
EUR 565
Description: Mouse monoclonal Leptin antibody

Leptin antibody
10R-L116A 500 ug
EUR 273
Description: Mouse monoclonal Leptin antibody

Leptin antibody
70R-6221 50 ug
EUR 467
Description: Rabbit polyclonal Leptin antibody raised against the N terminal of LEP

Leptin antibody
70R-6223 50 ug
EUR 467
Description: Rabbit polyclonal Leptin antibody raised against the middle region of LEP

Leptin antibody
70R-LG001 50 ug
EUR 273
Description: Affinity purified Goat polyclonal Leptin antibody

Leptin antibody
70R-LR002 100 ug
EUR 476
Description: Affinity purified Rabbit polyclonal Leptin antibody

Leptin antibody
70R-LR007 100 ug
EUR 327
Description: Affinity purified Rabbit polyclonal Leptin antibody

Leptin protein
30-AL12 1 mg
EUR 335
Description: Purified recombinant Human Leptin protein

Leptin protein
30R-1049 100 ug
EUR 300
Description: Purified recombinant Human Leptin protein

Leptin protein
30R-2359 1 mg
EUR 302
Description: Purified recombinant Human Leptin protein

Leptin protein
30R-2360 1 mg
EUR 317
Description: Purified recombinant Mouse Leptin protein

Leptin protein
30R-2361 1 mg
EUR 302
Description: Purified recombinant Human Leptin protein

Leptin protein
30R-3140 50 ug
EUR 257
Description: Purified recombinant Leptin protein

Leptin protein
30R-AL001 1 mg
EUR 273
Description: Purified recombinant Murin Leptin protein

Leptin protein
30R-AL018 1 mg
EUR 273
Description: Purified recombinant Human Leptin protein

Leptin antibody
70R-12330 100 ug
EUR 403
Description: Rabbit polyclonal Leptin antibody

Leptin antibody
70R-12331 100 ug
EUR 403
Description: Rabbit polyclonal Leptin antibody

Leptin antibody
70R-12332 100 ug
EUR 403
Description: Rabbit polyclonal Leptin antibody

Plain Glass Plates 2/pk, RM(MB-00)
2398232 10unit
EUR 241
Description: Rect angul ar wi t hout not ch & si de-spacer s

1 mm 21-well Combs 2/pk, RM
2398255 5unit
EUR 272

Mouse Leptin R(Leptin Receptor) ELISA Kit
EM0130 96T
EUR 524.1
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 18.75pg/ml

Human Leptin R(Leptin receptor) ELISA Kit
EH0217 96T
EUR 524.1
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 9.375pg/ml

1 mm Glass Plates 2/pk, RM(MAB-10)
2398230 2unit
EUR 249
Description: N ot ched pl at e wi t h 1 mm si de-spacer s

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