Comparative analysis of cell-free DNA content in culture

pharmacodane

Comparative analysis of cell-free DNA content in culture medium and mitochondrial DNA copy number in porcine parthenogenetically activated embryos

We examined the effect of ploidy on mitochondrial DNA (mtDNA) copy number in embryos and the amount of cell-free mitochondrial and nucleic DNA content (cf-mtDNA and cf-nDNA) in spent culture medium (SCM). Oocytes collected from the ovaries were matured, activated, incubated in medium containing cycloheximide (CHX) or CHX and cytochalasin B (CB) for 4.5 h to produce haploid or diploid embryos (H-group and D-group embryos).
These embryos were cultured for 7 days, and the blastocysts and SCM were examined. The amount of mtDNA and nDNA was determined by real-time PCR. The rate of development to the blastocyst stage was higher for the D-group than for the H-group. Moreover, D-group blastocysts had less mtDNA compared to the H-group blastocysts. After activation, the mitochondrial content was constant before the blastocyst stage in D-group embryos, but increased earlier in H-group embryos. The amount of cf-mtDNA in the SCM of D-group blastocysts was greater than that of H-group blastocysts.
However, when the cf-mtDNA in the SCM of 2 cell-stage embryos (day 2 post-activation) was examined, the amount of cf-mtDNA was greater in the H-group than in the D-group embryos. When D-group embryos were cultured for 7 days, a significant correlation was observed between the total cell number of blastocysts and cf-nDNA content in the SCM. Hence, although careful consideration is needed regarding the time point for evaluating mtDNA content in the embryos and SCM, this study demonstrates that mtDNA in the embryos and SCM was affected by the ploidy of the embryos.
pharmacodane

pharmacodane

rHu MIF

AK8255-0025 25µg Ask for price

rHu MIF

AK8255-0100 100µg Ask for price

rHu MIF

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rHu EGF

AK8327-0100 100µg Ask for price

rHu EGF

AK8327-0500 500µg Ask for price

rHu EGF

AK8327-1000 1mg Ask for price

rHu SHH

AK9088-0005 5µg Ask for price

rHu SHH

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rHu TPO

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AK9979-0020 20µg Ask for price

rHu MIG

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rHu MIG

AK9979-1000 1mg Ask for price

OSM

cyt-231 2µg
EUR 60
Description: Recombinant Human Oncostatin-M

OSM

CSB-CL017260HU 10 μg plasmid + 200μl Glycerol Ask for price

rHu aFGF

AK8230-0010 10µg Ask for price

rHu aFGF

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rHu BDNF

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rHu CNTF

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rHu GDNF

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rHu BAFF

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rHu TSLP

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rHu TRAIL

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rHu TRAIL

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rHu TRAIL

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rHu TRAIL

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rHu Leptin

AK8265-0200 200µg Ask for price

rHu Leptin

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rHu Leptin

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rHu RANTES

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rHu Insulin

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rHu Insulin

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rHu Artemin

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rHu Omentin

AK9083-0002 2µg Ask for price

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rHu Eotaxin

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rHu Visfatin

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AK9084-0002 2µg Ask for price

Fast compressive lens-free tomography for 3D biological cell culture imaging

We present a compressive lens-free technique that performs tomographic imaging across a cubic millimeter-scale volume from highly sparse data. Compared with existing lens-free 3D microscopy systems, our method requires an order of magnitude fewer multi-angle illuminations for tomographic reconstruction, leading to a compact, cost-effective and scanning-free setup with a reduced data acquisition time to enable high-throughput 3D imaging of dynamic biological processes.
We apply a fast proximal gradient algorithm with composite regularization to address the ill-posed tomographic inverse problem. Using simulated data, we show that the proposed method can achieve a reconstruction speed ∼10× faster than the state-of-the-art inverse problem approach in 3D lens-free microscopy. We experimentally validate the effectiveness of our method by imaging a resolution test chart and polystyrene beads, demonstrating its capability to resolve micron-size features in both lateral and axial directions.
Furthermore, tomographic reconstruction results of neuronspheres and intestinal organoids reveal the potential of this 3D imaging technique for high-resolution and high-throughput biological applications.

Adipose Tissue from Lean and Obese Mice Induces a Mesenchymal to Epithelial Transition-Like Effect in Triple Negative Breast Cancers Cells Grown in 3-Dimensional Culture

Breast cancer is the second leading cause of cancer-related mortality among women globally with obesity being one risk factor. Obese breast cancer patients have at least a 30% increased risk of death from breast cancer compared to non-obese breast cancer patients because they present with larger tumors and generally have increased rates of metastasis.
Moreover, obese breast cancer patients respond more poorly to treatment compared to non-obese patients, particularly pre-menopausal women diagnosed with triple negative breast cancer (TNBC). To help understand the molecular mechanisms underlying the increased metastasis associated with obesity, we previously established a three-dimensional culture system that permits the co-culture of adipocytes and TNBC cells in a manner that mimics an in vivo milieu.
Using this system, we demonstrate that white adipose tissue from both lean and obese mice can induce a partial mesenchymal-to-epithelial transition (MET). Triple negative breast cancer cells adopt an epithelial morphology and have an increased expression of some epithelial markers, but they maintain the expression of mesenchymal markers, furnishing the breast cancer cells with hybrid properties that are associated with more aggressive tumors.
Thus, these data suggest that adipose tissue has the potential to promote secondary tumor formation in lean and obese women. Further work is needed to determine if targeting the partial MET induced by adipose tissue could reduce metastasis.

Leptin

GWB-531D0A 1 mg Ask for price

Leptin

cyt-228 200µg
EUR 60
Description: Recombinant Human Leptin

Leptin

cyt-683 200µg
EUR 60
Description: Human Leptin

Leptin

DEE007 96
EUR 309

Leptin

AP84730 1mg
EUR 2640

Leptin

AP84748 1mg
EUR 2640

Leptin

AP84844 1mg
EUR 2640

Leptin

AP85016 1mg
EUR 2640

Leptin

AP85329 1mg
EUR 2640

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AP78370 1mg
EUR 2640

Leptin

AP78371 1mg
EUR 2640

Leptin

ant-172 0.5mg
EUR 275
Description: Mouse Anti Human Leptin

Leptin

RA21019 50 ug
EUR 522

Leptin

RA30011 50 ug
EUR 726

Mouse Leptin R(Leptin Receptor) ELISA Kit

EM0130 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 18.75pg/ml

Human Leptin R(Leptin receptor) ELISA Kit

EH0217 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 9.375pg/ml

Human Leptin R(Leptin receptor) ELISA Kit

EKF57080-48T 48T
EUR 396.9

Human Leptin R(Leptin receptor) ELISA Kit

EKF57080-5x96T 5x96T
EUR 2693.25

Human Leptin R(Leptin receptor) ELISA Kit

EKF57080-96T 96T
EUR 567

Leptin tA

cyt-352 10µg
EUR 60
Description: Recombinant Human Leptin Triple Antagonist

Leptin qA

cyt-353 10µg
EUR 60
Description: Recombinant Human Leptin Quadruple Antagonist

Leptin-A

cyt-1109 20µg
EUR 100
Description: Recombinant Tilapia Leptin-A

Leptin-B

cyt-1110 20µg
EUR 100
Description: Recombinant Tilapia Leptin-B

Leptin His

cyt-287 5µg
EUR 60
Description: Recombinant Human Leptin, His Tag

rHu Leptin

AK8265-0200 200µg Ask for price

rHu Leptin

AK8265-1000 1mg Ask for price

rHu Leptin

AK8265-5000 5mg Ask for price

Leptin rat

JP27295 96
EUR 611

Leptin, Rat

HY-P7233 50ug
EUR 183.6

OPPA01996-200UG - Leptin Human - Human Leptin Protein

OPPA01996-200UG 200ug
EUR 70

Leptin N82K

cyt-1107 50µg
EUR 60
Description: Recombinant Human Leptin N82K

Human Leptin

7-01138 200µg Ask for price

Human Leptin

7-01139 1mg Ask for price

Human Leptin

7-01140 5mg Ask for price

Leptin (Human)

MO15019 500 ug
EUR 1092

Leptin mouse

JP27160 96
EUR 611

Human Leptin

GWB-CAD18F 1 mg Ask for price

Leptin, Human

HY-P7232 100ug
EUR 224.4

Leptin-Rat

PR27100 200 ug
EUR 229.2

Leptin-Pig

PR27137 20 ug
EUR 229.2

Leptin, human

RC412-17 5mg
EUR 588.96

Leptin-Rat

PR27100-B each
EUR 184.5

Leptin-Pig

PR27137-B each
EUR 184.5

Human Leptin

cyt-683-1mg 1mg
EUR 145

Human Leptin

cyt-683-200g 200µg
EUR 60

Human Leptin

cyt-683-5mg 5mg
EUR 450

Human Leptin

rAP-2360 Inquiry Ask for price

Leptin protein

30-AL12 1 mg
EUR 312
Description: Purified recombinant Human Leptin protein

Leptin protein

30R-1049 100 ug
EUR 252
Description: Purified recombinant Human Leptin protein

Leptin protein

30R-2359 1 mg
EUR 165
Description: Purified recombinant Human Leptin protein

Leptin protein

30R-2360 1 mg
EUR 175
Description: Purified recombinant Mouse Leptin protein

Leptin protein

30R-2361 1 mg
EUR 165
Description: Purified recombinant Human Leptin protein

Leptin protein

30R-3140 50 ug
EUR 308.4
Description: Purified recombinant Leptin protein

Leptin protein

22061194-1 1 mg
EUR 459.5

Leptin protein

30R-AL001 1 mg
EUR 312
Description: Purified recombinant Murin Leptin protein

Leptin protein

30R-AL018 1 mg
EUR 312
Description: Purified recombinant Human Leptin protein

Leptin Protein

20-abx263119
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  • 100 ug
  • 1 mg
  • 20 ug

Leptin Protein

20-abx263120
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  • 100 ug
  • 1 mg
  • 20 ug

Leptin Protein

20-abx263121
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  • 100 ug
  • 1 mg
  • 20 ug

Leptin Protein

20-abx263122
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  • 100 ug
  • 1 mg
  • 20 ug

Leptin Protein

20-abx263123
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  • 100 ug
  • 1 mg
  • 20 ug

Leptin Protein

20-abx263124
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  • 100 ug
  • 1 mg
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Leptin Protein

20-abx263175
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  • 100 ug
  • 1 mg
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Leptin Protein

20-abx262112
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  • 1 mg
  • 200 ug
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Leptin Protein

20-abx262117
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  • 1 mg
  • 200 ug
  • 5 mg

Leptin Protein

20-abx262118
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  • 1 mg
  • 200 ug
  • 5 mg

Leptin Protein

20-abx262119
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  • 1 mg
  • 200 ug
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Leptin Protein

20-abx060295
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  • 100 ug
  • 1 mg

Leptin Protein

20-abx260622
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  • 1 mg
  • 25 ug
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Leptin-Human

PR27097 200 ug
EUR 229.2

Leptin-Mouse

PR27099 200 ug
EUR 229.2

Leptin-Human

PR27097-B each
EUR 184.5

Leptin-Mouse

PR27099-B each
EUR 184.5

Leptin Protein

abx263119-10mg 10 mg
EUR 512.5

Leptin Protein

abx263119-25mg 25 mg
EUR 2337.5

Leptin Protein

abx263119-5mg 5 mg
EUR 262.5

Leptin antibody

10-7820 500 ug
EUR 690
Description: Mouse monoclonal Leptin antibody

Leptin antibody

10-7821 500 ug
EUR 345
Description: Mouse monoclonal Leptin antibody

Leptin antibody

10-1757 200 ul
EUR 1475
Description: Mouse monoclonal Leptin antibody

Leptin antibody

10-1758 200 ul
EUR 1475
Description: Mouse monoclonal Leptin antibody

Leptin antibody

10-1759 200 ul
EUR 1475
Description: Mouse monoclonal Leptin antibody

Leptin antibody

10-2297 100 ug
EUR 489.6
Description: Recombinant Fab monoclonal Leptin antibody

Leptin antibody

10-2298 100 ug
EUR 489.6
Description: Recombinant Fab monoclonal Leptin antibody

Leptin antibody

10-2399 250 ug
EUR 166
Description: Mouse Monoclonal Leptin antibody

Leptin antibody

10-2400 250 ug
EUR 216
Description: Mouse Monoclonal Leptin antibody

Leptin antibody

10-2401 500 ug
EUR 277
Description: Mouse Monoclonal Leptin antibody

Leptin antibody

10-7822 500 ug
EUR 690
Description: Mouse monoclonal Leptin antibody

Leptin antibody

10R-7769 500 ug
EUR 678
Description: Mouse monoclonal Leptin antibody

Leptin antibody

10R-L116A 500 ug
EUR 240
Description: Mouse monoclonal Leptin antibody

Leptin Antibody

F44957-0.4ML 0.4 ml
EUR 322.15
Description: This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq].

Leptin Antibody

F49458-0.4ML 0.4 ml
EUR 322.15
Description: Leptin (LEP) is an adipocyte-secreted protein that regulates body fat and endocrine functions. Leptin is similar to the mouse obesity protein encoded by the obese gene (ob). In mouse studies, mutations in this gene are linked to severe and morbid obesity.

Leptin Receptor

E8ET1704-44 100ul
EUR 275
Description: Available in various conjugation types.

Leptin Receptor

GT15012 100 ug
EUR 631.2

Leptin Receptor

GT15059 100 ug
EUR 631.2

Leptin Antibody

GWB-2600AE 0.5 mg Ask for price

Leptin Antibody

GWB-2616CD 0.05 mg Ask for price

Leptin Antibody

GWB-2DC964 0.1 mg Ask for price

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GWB-565B0A 0.5 mg Ask for price

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GWB-58DD79 0.1 mg Ask for price

Leptin Antibody

GWB-3EAFF3 0.1 ml Ask for price

Leptin Antibody

DF8583 200ul
EUR 420

Leptin Antibody

DF8583-100ul 100ul
EUR 280

Leptin Antibody

DF8583-200ul 200ul
EUR 350

Leptin Antibody

E38PA6011 100ul
EUR 225
Description: Available in various conjugation types.

leptin antibody

E39-10463 100ug/100ul
EUR 225
Description: Available in various conjugation types.

Leptin Antibody

5366-100 each
EUR 379.2

Leptin Antibody

5366-30T each
EUR 175.2

Leptin Antibody

5367-100 each
EUR 379.2

Leptin Antibody

5367-30T each
EUR 175.2

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