Comparative analysis of cell-free DNA content in culture

      Ingen kommentarer til Comparative analysis of cell-free DNA content in culture
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Comparative analysis of cell-free DNA content in culture medium and mitochondrial DNA copy number in porcine parthenogenetically activated embryos

We examined the effect of ploidy on mitochondrial DNA (mtDNA) copy number in embryos and the amount of cell-free mitochondrial and nucleic DNA content (cf-mtDNA and cf-nDNA) in spent culture medium (SCM). Oocytes collected from the ovaries were matured, activated, incubated in medium containing cycloheximide (CHX) or CHX and cytochalasin B (CB) for 4.5 h to produce haploid or diploid embryos (H-group and D-group embryos).
These embryos were cultured for 7 days, and the blastocysts and SCM were examined. The amount of mtDNA and nDNA was determined by real-time PCR. The rate of development to the blastocyst stage was higher for the D-group than for the H-group. Moreover, D-group blastocysts had less mtDNA compared to the H-group blastocysts. After activation, the mitochondrial content was constant before the blastocyst stage in D-group embryos, but increased earlier in H-group embryos. The amount of cf-mtDNA in the SCM of D-group blastocysts was greater than that of H-group blastocysts.
However, when the cf-mtDNA in the SCM of 2 cell-stage embryos (day 2 post-activation) was examined, the amount of cf-mtDNA was greater in the H-group than in the D-group embryos. When D-group embryos were cultured for 7 days, a significant correlation was observed between the total cell number of blastocysts and cf-nDNA content in the SCM. Hence, although careful consideration is needed regarding the time point for evaluating mtDNA content in the embryos and SCM, this study demonstrates that mtDNA in the embryos and SCM was affected by the ploidy of the embryos.
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pharmacodane

OSM
MBS517355-02mg 0.2mg
EUR 1920

OSM
MBS517355-1mg 1mg
EUR 4835

OSM
MBS517355-5x1mg 5x1mg
EUR 21495

OSM
MBS553688-0002mg 0.002mg
EUR 210

OSM
MBS553688-001mg 0.01mg
EUR 290

OSM
MBS553688-1mg 1mg
EUR 5695

OSM
MBS9125368-002mL 0.02mL
EUR 200

OSM
MBS9125368-005mL 0.05mL
EUR 255

OSM
MBS9125368-01mL 0.1mL
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OSM
MBS9125368-02mL 0.2mL
EUR 545

OSM
MBS9125368-5x02mL 5x0.2mL
EUR 2265

OSM, CT (OSM, Oncostatin-M) (AP)
MBS6329746-02mL 0.2mL
EUR 980

OSM, CT (OSM, Oncostatin-M) (AP)
MBS6329746-5x02mL 5x0.2mL
EUR 4250

OSM, CT (OSM, Oncostatin-M) (PE)
MBS6329756-02mL 0.2mL
EUR 980

OSM, CT (OSM, Oncostatin-M) (PE)
MBS6329756-5x02mL 5x0.2mL
EUR 4250

OSM, CT (OSM, Oncostatin-M) (APC)
MBS6329747-02mL 0.2mL
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OSM, CT (OSM, Oncostatin-M) (APC)
MBS6329747-5x02mL 5x0.2mL
EUR 4250

OSM, CT (OSM, Oncostatin-M) (FITC)
MBS6329749-02mL 0.2mL
EUR 980

OSM, CT (OSM, Oncostatin-M) (FITC)
MBS6329749-5x02mL 5x0.2mL
EUR 4250

OSM, CT (OSM, Oncostatin-M) (Biotin)
MBS6329748-02mL 0.2mL
EUR 980

OSM, CT (OSM, Oncostatin-M) (Biotin)
MBS6329748-5x02mL 5x0.2mL
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rHu IL 2 , 3MIU
04-RHIL2-02F01 1 vial
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Description: Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated.

OSM (untagged)-Human oncostatin M (OSM)
SC121421 10 µg Ask for price

OSM, CT (OSM, Oncostatin-M) (MaxLight 405)
MBS6329751-01mL 0.1mL
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OSM, CT (OSM, Oncostatin-M) (MaxLight 405)
MBS6329751-5x01mL 5x0.1mL
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OSM, CT (OSM, Oncostatin-M) (MaxLight 490)
MBS6329752-01mL 0.1mL
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OSM, CT (OSM, Oncostatin-M) (MaxLight 490)
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OSM, CT (OSM, Oncostatin-M) (MaxLight 550)
MBS6329753-01mL 0.1mL
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OSM, CT (OSM, Oncostatin-M) (MaxLight 550)
MBS6329753-5x01mL 5x0.1mL
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OSM, CT (OSM, Oncostatin-M) (MaxLight 650)
MBS6329754-01mL 0.1mL
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OSM, CT (OSM, Oncostatin-M) (MaxLight 650)
MBS6329754-5x01mL 5x0.1mL
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OSM, CT (OSM, Oncostatin-M) (MaxLight 750)
MBS6329755-01mL 0.1mL
EUR 980

OSM, CT (OSM, Oncostatin-M) (MaxLight 750)
MBS6329755-5x01mL 5x0.1mL
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OSM, His
cyt-060 5µg
EUR 60
Description: Recombinant Human Oncostatin-M, His Tag

OSM, Rat
HY-P7105 100ug
EUR 1507.2

OSM (GFP-tagged) - Human oncostatin M (OSM)
RG204277 10 µg Ask for price

Osm (untagged) - Mouse oncostatin M (Osm), (10ug)
MC209127 10 µg Ask for price

OSM, Mouse
HY-P7275 50ug
EUR 914.4

pcDNA3.1(+)-OSM
PPL01674-2a 759bp
EUR 218
Description: pcDNA3.1(+)

OSM Antibody
37783 100ul
EUR 319

OSM Antibody
37783-100ul 100ul
EUR 302.4

OSM Antibody
1-CSB-PA934414
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  • 50ul
  • 100ul
Description: A polyclonal antibody against OSM. Recognizes OSM from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:2000, IHC:1:25-1:100

OSM Antibody
1-CSB-PA244255
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Description: A polyclonal antibody against OSM. Recognizes OSM from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:50-1:200

OSM Antibody
1-CSB-PA09694A0Rb
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  • 100ug
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Description: A polyclonal antibody against OSM. Recognizes OSM from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200

Osm Antibody
1-CSB-PA723970LA01RA
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Description: A polyclonal antibody against Osm. Recognizes Osm from Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IF; Recommended dilution: IF:1:50-1:200

OSM Antibody
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Description: OSM Rabbit Polyclonal Antibody

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Description: OSM Rabbit Polyclonal Antibody

OSM Antibody
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Description: Available in various conjugation types.

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Description: IHC,IF/ICC,ELISA(peptide)

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Description: IHC,IF/ICC,ELISA(peptide)

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MBS9629780-01mL 0.1mL
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MBS9632163-1mg 1mg
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osm-12 Antibody
CSB-PA244411XA01CXY-02mg 0.2mg Ask for price
Description: Recombinant Caenorhabditis elegans osm-12 protein

osm-12 Antibody
CSB-PA244411XA01CXY-10mg 10mg Ask for price
Description: Recombinant Caenorhabditis elegans osm-12 protein

OSM (209 a.a.)
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Description: Recombinant Human Oncostatin-M (209 a.a.)

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Description: Recombinant Human Oncostatin-M (195 a.a.)

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OSM (209a.a.), Human
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OSM, CT (OSM, Oncostatin-M) (Azide free) (HRP)
MBS6329750-02mL 0.2mL
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OSM, CT (OSM, Oncostatin-M) (Azide free) (HRP)
MBS6329750-5x02mL 5x0.2mL
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osm-8 Antibody
CSB-PA295214XA01CXY-02mg 0.2mg Ask for price
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osm-1 Antibody
CSB-PA635492XA01CXY-02mg 0.2mg Ask for price
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osm-1 Antibody
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osm-3 Antibody
CSB-PA343613XA01CXY-02mg 0.2mg Ask for price
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osm-3 Antibody
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Osm (GFP-tagged) - Mouse oncostatin M (Osm), (10ug)
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Osm (Myc-DDK-tagged) - Mouse oncostatin M (Osm)
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OSM (Myc-DDK-tagged)-Human oncostatin M (OSM)
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Osm (untagged ORF) - Rat oncostatin M (Osm), (10 ug)
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Recombinant Human VEGF165 (rHu VEGF165)
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EUR 235
Description: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human umbilical vein endothelial cells(HUVEC) is between 1.0-8.0 ng/ml.

Recombinant Human VEGF165 (rHu VEGF165)
MBS9419932-001mL 0.01mL
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Recombinant Human VEGF165 (rHu VEGF165)
MBS9419932-5x001mL 5x0.01mL
EUR 1120

Rat OSM siRNA
20-abx927424
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  • 15 nmol
  • 30 nmol

OSM-SMI-8
HY-148693 Get quote Ask for price
Description: OSM-SMI-8 (NSC642624) is a potent OSM (oncostatin M) antagonist. OSM-SMI-8 has the potential for the research of cancer[1].

OSM siRNA (Rat)
MBS8219448-15nmol 15nmol
EUR 405

OSM siRNA (Rat)
MBS8219448-30nmol 30nmol
EUR 565

OSM siRNA (Rat)
MBS8219448-5x30nmol 5x30nmol
EUR 2450

OSM Rabbit pAb
A6163-100ul 100 ul
EUR 369.6

OSM Rabbit pAb
A6163-200ul 200 ul
EUR 550.8

OSM Rabbit pAb
A6163-20ul 20 ul
EUR 219.6

OSM Rabbit pAb
A6163-50ul 50 ul
EUR 267.6

OSM cDNA Clone
MBS1272884-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

OSM cDNA Clone
MBS1272884-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

OSM Rabbit pAb
E2861991 100ul
EUR 225
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

OSM Rabbit pAb
E2251577 100ul
EUR 225
Description: Available in various conjugation types.

OSM Rabbit pAb
E2506163 100ul
EUR 225
Description: Available in various conjugation types.

OSM Rabbit pAb
MBS8540186-01mL 0.1mL
EUR 305

OSM Rabbit pAb
MBS8540186-01mLAF405L 0.1mL(AF405L)
EUR 565

OSM Rabbit pAb
MBS8540186-01mLAF405S 0.1mL(AF405S)
EUR 565

OSM Rabbit pAb
MBS8540186-01mLAF610 0.1mL(AF610)
EUR 565

OSM Rabbit pAb
MBS8540186-01mLAF635 0.1mL(AF635)
EUR 565

OSM Rabbit pAb
MBS8545936-01mL 0.1mL
EUR 305

OSM Rabbit pAb
MBS8545936-01mLAF405L 0.1mL(AF405L)
EUR 565

OSM Rabbit pAb
MBS8545936-01mLAF405S 0.1mL(AF405S)
EUR 565

OSM Rabbit pAb
MBS8545936-01mLAF610 0.1mL(AF610)
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OSM Rabbit pAb
MBS8545936-01mLAF635 0.1mL(AF635)
EUR 565

Human OSM siRNA
20-abx903778
  • Ask for price
  • Ask for price
  • 15 nmol
  • 30 nmol

Mouse OSM siRNA
20-abx927425
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  • Ask for price
  • 15 nmol
  • 30 nmol

Fast compressive lens-free tomography for 3D biological cell culture imaging

We present a compressive lens-free technique that performs tomographic imaging across a cubic millimeter-scale volume from highly sparse data. Compared with existing lens-free 3D microscopy systems, our method requires an order of magnitude fewer multi-angle illuminations for tomographic reconstruction, leading to a compact, cost-effective and scanning-free setup with a reduced data acquisition time to enable high-throughput 3D imaging of dynamic biological processes.
We apply a fast proximal gradient algorithm with composite regularization to address the ill-posed tomographic inverse problem. Using simulated data, we show that the proposed method can achieve a reconstruction speed ∼10× faster than the state-of-the-art inverse problem approach in 3D lens-free microscopy. We experimentally validate the effectiveness of our method by imaging a resolution test chart and polystyrene beads, demonstrating its capability to resolve micron-size features in both lateral and axial directions.
Furthermore, tomographic reconstruction results of neuronspheres and intestinal organoids reveal the potential of this 3D imaging technique for high-resolution and high-throughput biological applications.

Adipose Tissue from Lean and Obese Mice Induces a Mesenchymal to Epithelial Transition-Like Effect in Triple Negative Breast Cancers Cells Grown in 3-Dimensional Culture

Breast cancer is the second leading cause of cancer-related mortality among women globally with obesity being one risk factor. Obese breast cancer patients have at least a 30% increased risk of death from breast cancer compared to non-obese breast cancer patients because they present with larger tumors and generally have increased rates of metastasis.
Moreover, obese breast cancer patients respond more poorly to treatment compared to non-obese patients, particularly pre-menopausal women diagnosed with triple negative breast cancer (TNBC). To help understand the molecular mechanisms underlying the increased metastasis associated with obesity, we previously established a three-dimensional culture system that permits the co-culture of adipocytes and TNBC cells in a manner that mimics an in vivo milieu.
Using this system, we demonstrate that white adipose tissue from both lean and obese mice can induce a partial mesenchymal-to-epithelial transition (MET). Triple negative breast cancer cells adopt an epithelial morphology and have an increased expression of some epithelial markers, but they maintain the expression of mesenchymal markers, furnishing the breast cancer cells with hybrid properties that are associated with more aggressive tumors.
Thus, these data suggest that adipose tissue has the potential to promote secondary tumor formation in lean and obese women. Further work is needed to determine if targeting the partial MET induced by adipose tissue could reduce metastasis.

Leptin
ABS-022-11-04 400µl
EUR 1037.1
Description: Mouse

Leptin
ABS-022-11-1 1ml
EUR 2380.33
Description: Mouse

Leptin
ABS-022-11B-015 150µl
EUR 1193.29
Description: Mouse

Leptin
AP78370 1mg
EUR 2640

Leptin
AP78371 1mg
EUR 2640

Leptin
ant-172 0.5mg
EUR 275
Description: Mouse Anti Human Leptin

Leptin
AP84730 1mg
EUR 2640

Leptin
AP84748 1mg
EUR 2640

Leptin
AP84844 1mg
EUR 2640

Leptin
AP85016 1mg
EUR 2640

Leptin
AP85329 1mg
EUR 2640

Leptin
DEE007 96
EUR 309

Leptin
cyt-683 200µg
EUR 60
Description: Human Leptin

Leptin
cyt-228 200µg
EUR 60
Description: Recombinant Human Leptin

LEPTIN
GWB-52C816 1 mg Ask for price

Leptin
GWB-531D0A 1 mg Ask for price

Leptin
GT15011 100 ug
EUR 631.2

Leptin
E21-067 10ug
EUR 225
Description: Recombinant Human Leptin is produced by our E.coli expression system and the target gene encoding Val22-Cys167 is expressed.

Leptin
E8ER1912-27 100ul
EUR 275
Description: Available in various conjugation types.

Leptin
MBS355750-01mg 0.1mg
EUR 700

Leptin
MBS355750-5x01mg 5x0.1mg
EUR 2855

Leptin
MBS355805-01mg 0.1mg
EUR 335

Leptin
MBS355805-1mg 1mg
EUR 1060

Leptin
MBS355805-5x1mg 5x1mg
EUR 4460

Leptin
MBS556392-005mg 0.05mg
EUR 500

Leptin
MBS556392-5x005mg 5x0.05mg
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Leptin
MBS556419-005mg 0.05mg
EUR 610

Leptin
MBS556419-5x005mg 5x0.05mg
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Leptin
MBS692655-01mg 0.1mg
EUR 725

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MBS692655-5x01mg 5x0.1mg
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Leptin
MBS8577915-01mL 0.1mL
EUR 345

Leptin
MBS8577915-01mLAF405L 0.1mL(AF405L)
EUR 565

Leptin
MBS8577915-01mLAF405S 0.1mL(AF405S)
EUR 565

Leptin
MBS8577915-01mLAF610 0.1mL(AF610)
EUR 565

Leptin
MBS8577915-01mLAF635 0.1mL(AF635)
EUR 565

Leptin
MBS8532855-001mg 0.01mg
EUR 305

Leptin
MBS8532855-5x001mg 5x0.01mg
EUR 1220

Leptin
RA21019 50 ug
EUR 341.55

Leptin
RA30011 50 ug
EUR 391.5

Human Leptin R(Leptin receptor) ELISA Kit
EKF57080-48T 48T
EUR 396.9

Human Leptin R(Leptin receptor) ELISA Kit
EKF57080-5x96T 5x96T
EUR 2693.25

Human Leptin R(Leptin receptor) ELISA Kit
EKF57080-96T 96T
EUR 567

Human Leptin R(Leptin receptor) ELISA Kit
EH0217 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 9.375pg/ml

Mouse Leptin R(Leptin Receptor) ELISA Kit
EM0130 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 18.75pg/ml

Leptin (LEP)
MBS517173-02mg 0.2mg
EUR 235

Leptin (LEP)
MBS517173-1mg 1mg
EUR 350

Leptin (LEP)
MBS517173-2mg 2mg
EUR 445

Leptin (LEP)
MBS517173-5x2mg 5x2mg
EUR 1745

Leptin tA
cyt-352 10µg
EUR 60
Description: Recombinant Human Leptin Triple Antagonist

Leptin qA
cyt-353 10µg
EUR 60
Description: Recombinant Human Leptin Quadruple Antagonist

Leptin/OB
MBS200499-005mL 0.05mL
EUR 275

Leptin/OB
MBS200499-01mL 0.1mL
EUR 360

Leptin/OB
MBS200499-5x01mL 5x0.1mL
EUR 1375

Leptin (PE)
MBS6251979-01mL 0.1(mL
EUR 995

Leptin (PE)
MBS6251979-5x01mL 5x0.1mL
EUR 4325

Leptin (AP)
MBS6248093-01mL 0.1(mL
EUR 995

Leptin (AP)
MBS6248093-5x01mL 5x0.1mL
EUR 4325

OPPA01996-200UG - Leptin Human - Human Leptin Protein
OPPA01996-200UG 200ug
EUR 70

Leptin-A
cyt-1109 20µg
EUR 100
Description: Recombinant Tilapia Leptin-A

Leptin-B
cyt-1110 20µg
EUR 100
Description: Recombinant Tilapia Leptin-B

Leptin His
cyt-287 5µg
EUR 60
Description: Recombinant Human Leptin, His Tag

Leptin, Rat
HY-P7233 50ug
EUR 183.6

Leptin rat
JP27295 96
EUR 611

Leptin (HRP)
MBS6251029-01mL 0.1(mL
EUR 995

Leptin (HRP)
MBS6251029-5x01mL 5x0.1mL
EUR 4325

Leptin (APC)
MBS6268947-01mL 0.1mL
EUR 995

Leptin (APC)
MBS6268947-5x01mL 5x0.1mL
EUR 4325

Rat Leptin
MBS692099-02mg 0.2mg
EUR 300

Rat Leptin
MBS692099-1mg 1mg
EUR 450

Rat Leptin
MBS692099-5x1mg 5x1mg
EUR 1725

Rat leptin
MBS400026-01mg 0.1mg
EUR 170

Rat leptin
MBS400026-05mg 0.5mg
EUR 195

Rat leptin
MBS400026-10mg 10mg
EUR 1035

Rat leptin
MBS400026-1mg 1mg
EUR 245

Rat leptin
MBS400026-5mg 5mg
EUR 660

Dog leptin
MBS400028-01mg 0.1mg
EUR 220

Dog leptin
MBS400028-05mg 0.5mg
EUR 545

Dog leptin
MBS400028-10mg 10mg
EUR 4765

Dog leptin
MBS400028-1mg 1mg
EUR 845

Dog leptin
MBS400028-5mg 5mg
EUR 3080

Leptin N82K
cyt-1107 50µg
EUR 60
Description: Recombinant Human Leptin N82K

Leptin (FITC)
MBS6250025-01mL 0.1(mL
EUR 995

Leptin (FITC)
MBS6250025-5x01mL 5x0.1mL
EUR 4325

Human Leptin
7-01138 200µg Ask for price

Human Leptin
7-01139 1mg Ask for price

Human Leptin
7-01140 5mg Ask for price

Human Leptin
cyt-683-1mg 1mg
EUR 145

Human Leptin
cyt-683-200g 200µg
EUR 60

Human Leptin
cyt-683-5mg 5mg
EUR 450

Leptin, Human
HY-P7232 100ug
EUR 224.4

Human Leptin
GWB-CAD18F 1 mg Ask for price

Leptin mouse
JP27160 96
EUR 611

Human Leptin
MBS143680-02mg 0.2mg
EUR 240

Human Leptin
MBS143680-1mg 1mg
EUR 310

Human Leptin
MBS143680-5mg 5mg
EUR 610

Human Leptin
MBS143680-5x5mg 5x5mg
EUR 2405

Mouse Leptin
MBS691546-02mg 0.2mg
EUR 300

Mouse Leptin
MBS691546-1mg 1mg
EUR 450

Mouse Leptin
MBS691546-5x1mg 5x1mg
EUR 1725

Human Leptin
MBS691942-02mg 0.2mg
EUR 300

Human Leptin
MBS691942-1mg 1mg
EUR 450

Human Leptin
MBS691942-5x1mg 5x1mg
EUR 1725

Leptin (Human)
MO15019 500 ug
EUR 1092

Leptin-Rat
PR27100 200 ug
EUR 97.2

Leptin-Rat
PR27100-B each
EUR 184.5

Leptin-Pig
PR27137 20 ug
EUR 97.2

Leptin-Pig
PR27137-B each
EUR 184.5

Horse leptin
MBS400029-01mg 0.1mg
EUR 220

Horse leptin
MBS400029-05mg 0.5mg
EUR 545

Horse leptin
MBS400029-10mg 10mg
EUR 4765

Horse leptin
MBS400029-1mg 1mg
EUR 845

Horse leptin
MBS400029-5mg 5mg
EUR 3080

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