Comparative analysis of cell-free DNA content in culture

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Comparative analysis of cell-free DNA content in culture medium and mitochondrial DNA copy number in porcine parthenogenetically activated embryos

We examined the effect of ploidy on mitochondrial DNA (mtDNA) copy number in embryos and the amount of cell-free mitochondrial and nucleic DNA content (cf-mtDNA and cf-nDNA) in spent culture medium (SCM). Oocytes collected from the ovaries were matured, activated, incubated in medium containing cycloheximide (CHX) or CHX and cytochalasin B (CB) for 4.5 h to produce haploid or diploid embryos (H-group and D-group embryos).
These embryos were cultured for 7 days, and the blastocysts and SCM were examined. The amount of mtDNA and nDNA was determined by real-time PCR. The rate of development to the blastocyst stage was higher for the D-group than for the H-group. Moreover, D-group blastocysts had less mtDNA compared to the H-group blastocysts. After activation, the mitochondrial content was constant before the blastocyst stage in D-group embryos, but increased earlier in H-group embryos. The amount of cf-mtDNA in the SCM of D-group blastocysts was greater than that of H-group blastocysts.
However, when the cf-mtDNA in the SCM of 2 cell-stage embryos (day 2 post-activation) was examined, the amount of cf-mtDNA was greater in the H-group than in the D-group embryos. When D-group embryos were cultured for 7 days, a significant correlation was observed between the total cell number of blastocysts and cf-nDNA content in the SCM. Hence, although careful consideration is needed regarding the time point for evaluating mtDNA content in the embryos and SCM, this study demonstrates that mtDNA in the embryos and SCM was affected by the ploidy of the embryos.
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pharmacodane

Recombinant Human Oncostatin-M (rHu OSM)

MBS9419927-5x001mL 5x0.01mL
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Fast compressive lens-free tomography for 3D biological cell culture imaging

We present a compressive lens-free technique that performs tomographic imaging across a cubic millimeter-scale volume from highly sparse data. Compared with existing lens-free 3D microscopy systems, our method requires an order of magnitude fewer multi-angle illuminations for tomographic reconstruction, leading to a compact, cost-effective and scanning-free setup with a reduced data acquisition time to enable high-throughput 3D imaging of dynamic biological processes.
We apply a fast proximal gradient algorithm with composite regularization to address the ill-posed tomographic inverse problem. Using simulated data, we show that the proposed method can achieve a reconstruction speed ∼10× faster than the state-of-the-art inverse problem approach in 3D lens-free microscopy. We experimentally validate the effectiveness of our method by imaging a resolution test chart and polystyrene beads, demonstrating its capability to resolve micron-size features in both lateral and axial directions.
Furthermore, tomographic reconstruction results of neuronspheres and intestinal organoids reveal the potential of this 3D imaging technique for high-resolution and high-throughput biological applications.

Adipose Tissue from Lean and Obese Mice Induces a Mesenchymal to Epithelial Transition-Like Effect in Triple Negative Breast Cancers Cells Grown in 3-Dimensional Culture

Breast cancer is the second leading cause of cancer-related mortality among women globally with obesity being one risk factor. Obese breast cancer patients have at least a 30% increased risk of death from breast cancer compared to non-obese breast cancer patients because they present with larger tumors and generally have increased rates of metastasis.
Moreover, obese breast cancer patients respond more poorly to treatment compared to non-obese patients, particularly pre-menopausal women diagnosed with triple negative breast cancer (TNBC). To help understand the molecular mechanisms underlying the increased metastasis associated with obesity, we previously established a three-dimensional culture system that permits the co-culture of adipocytes and TNBC cells in a manner that mimics an in vivo milieu.
Using this system, we demonstrate that white adipose tissue from both lean and obese mice can induce a partial mesenchymal-to-epithelial transition (MET). Triple negative breast cancer cells adopt an epithelial morphology and have an increased expression of some epithelial markers, but they maintain the expression of mesenchymal markers, furnishing the breast cancer cells with hybrid properties that are associated with more aggressive tumors.
Thus, these data suggest that adipose tissue has the potential to promote secondary tumor formation in lean and obese women. Further work is needed to determine if targeting the partial MET induced by adipose tissue could reduce metastasis.

Leptin

E8ER1912-27 100ul
EUR 275
Description: Available in various conjugation types.

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AP78370 1mg
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Leptin

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AP84844 1mg
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AP85016 1mg
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ant-172 0.5mg
EUR 275
Description: Mouse Anti Human Leptin

LEPTIN

GWB-52C816 1 mg Ask for price

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GT15011 100 ug
EUR 631.2

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Leptin

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MBS556419-005mg 0.05mg
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MBS556419-5x005mg 5x0.05mg
EUR 2590

Human Leptin R(Leptin receptor) ELISA Kit

EH0217 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 9.375pg/ml

Human Leptin R(Leptin receptor) ELISA Kit

EKF57080-48T 48T
EUR 396.9

Human Leptin R(Leptin receptor) ELISA Kit

EKF57080-5x96T 5x96T
EUR 2693.25

Human Leptin R(Leptin receptor) ELISA Kit

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Mouse Leptin R(Leptin Receptor) ELISA Kit

EM0130 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 18.75pg/ml

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MBS517173-02mg 0.2mg
EUR 235

Leptin (LEP)

MBS517173-1mg 1mg
EUR 350

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MBS517173-2mg 2mg
EUR 445

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MBS517173-5x2mg 5x2mg
EUR 1745

OPPA01996-200UG - Leptin Human - Human Leptin Protein

OPPA01996-200UG 200ug
EUR 70

Leptin tA

cyt-352 10µg
EUR 60
Description: Recombinant Human Leptin Triple Antagonist

Leptin qA

cyt-353 10µg
EUR 60
Description: Recombinant Human Leptin Quadruple Antagonist

Leptin (AP)

MBS6248093-01mL 0.1(mL
EUR 995

Leptin (AP)

MBS6248093-5x01mL 5x0.1mL
EUR 4325

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MBS6251979-01mL 0.1(mL
EUR 995

Leptin (PE)

MBS6251979-5x01mL 5x0.1mL
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Leptin/OB

MBS200499-005mL 0.05mL
EUR 275

Leptin/OB

MBS200499-01mL 0.1mL
EUR 360

Leptin/OB

MBS200499-5x01mL 5x0.1mL
EUR 1375

Leptin-A

cyt-1109 20µg
EUR 100
Description: Recombinant Tilapia Leptin-A

Leptin-B

cyt-1110 20µg
EUR 100
Description: Recombinant Tilapia Leptin-B

Leptin His

cyt-287 5µg
EUR 60
Description: Recombinant Human Leptin, His Tag

rHu Leptin

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rHu Leptin

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rHu Leptin

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Leptin, Rat

HY-P7233 50ug
EUR 183.6

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JP27295 96
EUR 611

Rat Leptin

MBS692099-02mg 0.2mg
EUR 300

Rat Leptin

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EUR 450

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EUR 1725

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EUR 845

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EUR 3080

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EUR 4325

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Leptin (APC)

MBS6268947-5x01mL 5x0.1mL
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Leptin N82K

cyt-1107 50µg
EUR 60
Description: Recombinant Human Leptin N82K

Leptin (FITC)

MBS6250025-01mL 0.1(mL
EUR 995

Leptin (FITC)

MBS6250025-5x01mL 5x0.1mL
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7-01138 200µg Ask for price

Human Leptin

7-01139 1mg Ask for price

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7-01140 5mg Ask for price

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HY-P7232 100ug
EUR 224.4

Human Leptin

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PR27100 200 ug
EUR 229.2

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PR27137 20 ug
EUR 229.2

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JP27160 96
EUR 611

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MO15019 500 ug
EUR 1092

Leptin, human

RC412-17 5mg
EUR 588.96

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PR27100-B each
EUR 184.5

Leptin-Pig

PR27137-B each
EUR 184.5

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rAP-2360 Inquiry Ask for price

Human Leptin

cyt-683-1mg 1mg
EUR 145

Human Leptin

cyt-683-200g 200µg
EUR 60

Human Leptin

cyt-683-5mg 5mg
EUR 450

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MBS691546-02mg 0.2mg
EUR 300

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MBS691546-1mg 1mg
EUR 450

Mouse Leptin

MBS691546-5x1mg 5x1mg
EUR 1725

Human Leptin

MBS691942-02mg 0.2mg
EUR 300

Human Leptin

MBS691942-1mg 1mg
EUR 450

Human Leptin

MBS691942-5x1mg 5x1mg
EUR 1725

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MBS143680-02mg 0.2mg
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MBS143680-5x5mg 5x5mg
EUR 2405

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MBS400029-1mg 1mg
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MBS400029-5mg 5mg
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MBS400030-01mg 0.1mg
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MBS400030-05mg 0.5mg
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