Biocompliant Composite Au/pHEMA Plasmonic Scaffolds for 3D Cell Culture and Noninvasive Sensing of Cellular Metabolites
The field of 3D printing is an area of active research, with a substantial focus given to the design and construction of customized tools for applications in technology. There exists a particular need in these developing areas of opportunity for new multi-functional soft materials that are biologically compatible for the growth and directed culturing of cells. Herein, a composite material consisting of gold nanoparticles with useful plasmonic properties embedded within a highly hydrophilic poly-2-hydroxyethylmethacrylate matrix is described and characterized.
This composite material serves dual functions as both host framework scaffold for cell lines such as pre-osteoblasts as well as a plasmonic biosensor for in situ measurements of living cells. The plasmonic properties of this system are characterized as a function of the material properties and related to compositional features of the material through a proposed light-directed mechanism.
This chemistry provides a tunable, 3D printable plasmonic composite material of encapsulated gold nanoparticles in a biologically-compliant, acrylate-based hydrogel matrix. Surface-enhanced Raman scattering studies of 3D-microcultures supported by the scaffolds are carried out and the strong influence of perm-selective molecular diffusion in its analytical responses is established. Most notably, specific, largely hydrophilic, cellular metabolites are detected within the supported live cultures.
pharmacodane
anti-Activator of basal transcription 1 |
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| YF-PA18596 | Abfrontier | 50 ul | EUR 363 |
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Description: Mouse polyclonal to Activator of basal transcription 1 |
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anti-Activator of basal transcription 1 |
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| YF-PA18597 | Abfrontier | 50 ug | EUR 363 |
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Description: Mouse polyclonal to Activator of basal transcription 1 |
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anti-Activator of basal transcription 1 |
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| YF-PA18598 | Abfrontier | 50 ul | EUR 363 |
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Description: Mouse polyclonal to Activator of basal transcription 1 |
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anti-Activator of basal transcription 1 |
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| YF-PA18599 | Abfrontier | 50 ug | EUR 363 |
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Description: Mouse polyclonal to Activator of basal transcription 1 |
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Activator of Basal Transcription 1 (ABT1) Antibody |
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| 20-abx110789 | Abbexa |
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Activator Of Basal Transcription 1 (ABT1) Antibody |
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| 20-abx338734 | Abbexa |
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Activator of Basal Transcription 1 (ABT1) Antibody |
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| 20-abx004524 | Abbexa |
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Activator of Basal Transcription 1 (ABT1) Antibody |
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| abx037021-100ug | Abbexa | 100 ug | EUR 391 |
Activator of Basal Transcription 1 (ABT1) Antibody |
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| abx230056-100ug | Abbexa | 100 ug | EUR 481 |
Activator Of Basal Transcription 1 (ABT1) Antibody (HRP) |
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| 20-abx334566 | Abbexa |
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Activator Of Basal Transcription 1 (ABT1) Antibody (FITC) |
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| 20-abx334567 | Abbexa |
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Activator Of Basal Transcription 1 (ABT1) Antibody (Biotin) |
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| 20-abx334568 | Abbexa |
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Human Activator Of Basal Transcription 1 (ABT1) ELISA Kit |
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| abx385562-96tests | Abbexa | 96 tests | EUR 911 |
Bovine Activator of basal transcription 1, ABT1 ELISA KIT |
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| ELI-24538b | Lifescience Market | 96 Tests | EUR 928 |
Mouse Activator of basal transcription 1, Abt1 ELISA KIT |
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| ELI-24666m | Lifescience Market | 96 Tests | EUR 865 |
Human Activator of basal transcription 1, ABT1 ELISA KIT |
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| ELI-24776h | Lifescience Market | 96 Tests | EUR 824 |
Human Activator of basal transcription 1(ABT1) ELISA kit |
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| E01A1165-192T | BlueGene | 192 tests | EUR 1270 |
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Description: A sandwich ELISA for quantitative measurement of Human Activator of basal transcription 1(ABT1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Human Activator of basal transcription 1(ABT1) ELISA kit |
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| E01A1165-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
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Description: A sandwich ELISA for quantitative measurement of Human Activator of basal transcription 1(ABT1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Human Activator of basal transcription 1(ABT1) ELISA kit |
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| E01A1165-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
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Description: A sandwich ELISA for quantitative measurement of Human Activator of basal transcription 1(ABT1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rabbit Activator of basal transcription 1(ABT1) ELISA kit |
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| E04A1165-192T | BlueGene | 192 tests | EUR 1270 |
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Description: A sandwich ELISA for quantitative measurement of Rabbit Activator of basal transcription 1(ABT1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rabbit Activator of basal transcription 1(ABT1) ELISA kit |
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| E04A1165-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
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Description: A sandwich ELISA for quantitative measurement of Rabbit Activator of basal transcription 1(ABT1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rabbit Activator of basal transcription 1(ABT1) ELISA kit |
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| E04A1165-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
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Description: A sandwich ELISA for quantitative measurement of Rabbit Activator of basal transcription 1(ABT1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse Activator of basal transcription 1(ABT1) ELISA kit |
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| E03A1165-192T | BlueGene | 192 tests | EUR 1270 |
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Description: A sandwich ELISA for quantitative measurement of Mouse Activator of basal transcription 1(ABT1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse Activator of basal transcription 1(ABT1) ELISA kit |
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| E03A1165-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
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Description: A sandwich ELISA for quantitative measurement of Mouse Activator of basal transcription 1(ABT1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse Activator of basal transcription 1(ABT1) ELISA kit |
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| E03A1165-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
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Description: A sandwich ELISA for quantitative measurement of Mouse Activator of basal transcription 1(ABT1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Activator of basal transcription 1(ABT1) ELISA kit |
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| E02A1165-192T | BlueGene | 192 tests | EUR 1270 |
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Description: A sandwich ELISA for quantitative measurement of Rat Activator of basal transcription 1(ABT1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Activator of basal transcription 1(ABT1) ELISA kit |
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| E02A1165-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
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Description: A sandwich ELISA for quantitative measurement of Rat Activator of basal transcription 1(ABT1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Activator of basal transcription 1(ABT1) ELISA kit |
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| E02A1165-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
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Description: A sandwich ELISA for quantitative measurement of Rat Activator of basal transcription 1(ABT1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Pig Activator of basal transcription 1(ABT1) ELISA kit |
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| E07A1165-192T | BlueGene | 192 tests | EUR 1270 |
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Description: A sandwich ELISA for quantitative measurement of Porcine Activator of basal transcription 1(ABT1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Pig Activator of basal transcription 1(ABT1) ELISA kit |
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| E07A1165-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
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Description: A sandwich ELISA for quantitative measurement of Porcine Activator of basal transcription 1(ABT1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Pig Activator of basal transcription 1(ABT1) ELISA kit |
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| E07A1165-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
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Description: A sandwich ELISA for quantitative measurement of Porcine Activator of basal transcription 1(ABT1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Goat Activator of basal transcription 1(ABT1) ELISA kit |
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| E06A1165-192T | BlueGene | 192 tests | EUR 1270 |
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Description: A sandwich ELISA for quantitative measurement of Goat Activator of basal transcription 1(ABT1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Goat Activator of basal transcription 1(ABT1) ELISA kit |
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| E06A1165-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
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Description: A sandwich ELISA for quantitative measurement of Goat Activator of basal transcription 1(ABT1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Goat Activator of basal transcription 1(ABT1) ELISA kit |
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| E06A1165-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
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Description: A sandwich ELISA for quantitative measurement of Goat Activator of basal transcription 1(ABT1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog Activator of basal transcription 1(ABT1) ELISA kit |
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| E08A1165-192T | BlueGene | 192 tests | EUR 1270 |
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Description: A sandwich ELISA for quantitative measurement of Canine Activator of basal transcription 1(ABT1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog Activator of basal transcription 1(ABT1) ELISA kit |
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| E08A1165-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
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Description: A sandwich ELISA for quantitative measurement of Canine Activator of basal transcription 1(ABT1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog Activator of basal transcription 1(ABT1) ELISA kit |
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| E08A1165-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
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Description: A sandwich ELISA for quantitative measurement of Canine Activator of basal transcription 1(ABT1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Monkey Activator of basal transcription 1(ABT1) ELISA kit |
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| E09A1165-192T | BlueGene | 192 tests | EUR 1270 |
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Description: A sandwich ELISA for quantitative measurement of Monkey Activator of basal transcription 1(ABT1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Monkey Activator of basal transcription 1(ABT1) ELISA kit |
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| E09A1165-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
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Description: A sandwich ELISA for quantitative measurement of Monkey Activator of basal transcription 1(ABT1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Monkey Activator of basal transcription 1(ABT1) ELISA kit |
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| E09A1165-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
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Description: A sandwich ELISA for quantitative measurement of Monkey Activator of basal transcription 1(ABT1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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BME 100X Amino Acids for Basal Medium Eagle (Modified). W/O L-glutamine. |
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| BML02-500ML | Caisson Labs | 500 ml | EUR 104 |
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Description: 100X Amino Acids for Basal Medium Eagle (Modified). Without L-glutamine. |
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Guinea pig Activator of basal transcription 1(ABT1) ELISA kit |
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| E05A1165-192T | BlueGene | 192 tests | EUR 1270 |
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Description: A sandwich ELISA for quantitative measurement of Guinea pig Activator of basal transcription 1(ABT1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Guinea pig Activator of basal transcription 1(ABT1) ELISA kit |
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| E05A1165-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
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Description: A sandwich ELISA for quantitative measurement of Guinea pig Activator of basal transcription 1(ABT1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Guinea pig Activator of basal transcription 1(ABT1) ELISA kit |
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| E05A1165-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
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Description: A sandwich ELISA for quantitative measurement of Guinea pig Activator of basal transcription 1(ABT1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Pericyte Basal Media |
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| PGB002 | Neuromics | 475 ml | EUR 271 |
Astrocyte Basal Media |
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| PGB004 | Neuromics | 500 ml | EUR 271 |
ENDO Basal Media |
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| MED002 | Neuromics | 500 ml | EUR 207 |
Anderson's Basal Salt |
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| CP001-010 | GenDepot | 10x1L | EUR 99 |
Anderson's Basal Salt |
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| CP001-500 | GenDepot | 50L | EUR 126 |
Anderson's Basal Salt |
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| CP002-010 | GenDepot | 10X1L | EUR 99 |
Anderson's Basal Salt |
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| CP002-500 | GenDepot | 50L | EUR 126 |
500 ML GRACES'S INSECT BASAL MEDIUM (VAUGHN MOD.) WITH L-GLUTAMINE, WITHOUT INSECT HEMOLYMPH |
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| 13-200-CV | CORNING | 500 mL/pk | EUR 315 |
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Description: Specialty Media; Insect Media Products |
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M9CA Medium |
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| SD7025 | Bio Basic | 250g | EUR 71.75 |
YM Medium |
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| SD7031 | Bio Basic | 250g | EUR 70.45 |
TYGPN Medium |
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| SD7032 | Bio Basic | 250g | EUR 70.01 |
M63 Medium |
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| SD7033 | Bio Basic | 250g | EUR 71.75 |
M9 Medium |
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| SD7024 | Bio Basic | 250g | EUR 71.75 |
A Medium |
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| DJ1018 | Bio Basic | 100g | EUR 84.8 |
NeuroProgenitor Medium |
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| NM42400 | Neuromics | 125 ml | EUR 304 |
Heller's Medium |
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| CP014-010 | GenDepot | 10X1L | EUR 99 |
Heller's Medium |
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| CP014-500 | GenDepot | 50L | EUR 126 |
Hoagland's Medium |
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| CP015-010 | GenDepot | 10X1L | EUR 99 |
Hoagland's Medium |
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| CP015-500 | GenDepot | 50L | EUR 126 |
Advanced Medium |
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| C0003-04 | Addexbio | RT 500 mL Bottle | EUR 103 |
Medium 199 |
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| C0012-01 | Addexbio | RT 500 mL Bottle | EUR 105 |
DC MEDIUM |
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| D04-117-10kg | Alphabiosciences | 10 kg | EUR 1579 |
DC MEDIUM |
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| D04-117-2kg | Alphabiosciences | 2kg | EUR 382 |
DC MEDIUM |
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| D04-117-500g | Alphabiosciences | 500 g | EUR 140 |
HLP MEDIUM |
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| H08-107-10kg | Alphabiosciences | 10 kg | EUR 2278 |
HLP MEDIUM |
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| H08-107-2Kg | Alphabiosciences | 2 Kg | EUR 534 |
HLP MEDIUM |
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| H08-107-500g | Alphabiosciences | 500 g | EUR 182 |
EC MEDIUM |
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| E05-100-10kg | Alphabiosciences | 10 kg | EUR 814 |
EC MEDIUM |
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| E05-100-2Kg | Alphabiosciences | 2 Kg | EUR 216 |
EC MEDIUM |
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| E05-100-500g | Alphabiosciences | 500 g | EUR 95 |
COLIFORM MEDIUM |
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| C03-127-10kg | Alphabiosciences | 10 kg | EUR 1324 |
COLIFORM MEDIUM |
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| C03-127-2kg | Alphabiosciences | 2kg | EUR 327 |
COLIFORM MEDIUM |
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| C03-127-500g | Alphabiosciences | 500 g | EUR 125 |
SIM MEDIUM |
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| S19-110-10kg | Alphabiosciences | 10 kg | EUR 1021 |
SIM MEDIUM |
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| S19-110-2kg | Alphabiosciences | 2kg | EUR 261 |
SIM MEDIUM |
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| S19-110-500g | Alphabiosciences | 500 g | EUR 107 |
SOB MEDIUM |
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| S19-124-10kg | Alphabiosciences | 10 kg | EUR 965 |
SOB MEDIUM |
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| S19-124-2kg | Alphabiosciences | 2kg | EUR 249 |
SOB MEDIUM |
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| S19-124-500g | Alphabiosciences | 500 g | EUR 104 |
DKW Basal Salt Mixture |
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| CP007-010 | GenDepot | 10X1L | EUR 99 |
DKW Basal Salt Mixture |
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| CP007-500 | GenDepot | 50L | EUR 126 |
ExpressMax™ Basal Salts |
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| 145 | AthenaES | 500 g | EUR 86 |
Basal Body Marker antibody |
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| 10-2480 | Fitzgerald | 250 ug | EUR 492 |
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Description: Mouse monoclonal Basal Body Marker antibody |
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Basal Cell Cytokeratin antibody |
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| 10R-7959 | Fitzgerald | 100 ug | EUR 370 |
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Description: Mouse monoclonal Basal Cell Cytokeratin antibody |
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Insect Cell Medium: TNM-FH Insect Culture Medium |
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| ABP-MED-10001 | Allele Biotech | 1 liter | Ask for price |
Insect Cell Medium: Serum-Free Insect Culture Medium |
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| ABP-MED-10002 | Allele Biotech | 1 liter | Ask for price |
Insect Cell Medium: Grace?s Insect Medium (Unsupplemented) |
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| ABP-MED-10004 | Allele Biotech | 1 liter | Ask for price |
Cancer of the breast, basal-like breast carcinoma, 48 cases (1.5mm), set 1 |
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| BRC965 | Pantomics | 1 | EUR 410 |
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Description: Breast cancer tissue array, basal-like breast carcinoma, 48 cases (1.5mm), set 1, 96 cores, 48 cases, containing 35 cases basal-like, 3 cases luminal A, 3 cases luminal B, 4 cases Her2 overexpression types and 1 case Adenoid cystic carcinoma for positive control, with grading, TNM staging, and ER, PR, HER2, P53, EGFR, Ki67,CK5/6 and Nestin IHC data. |
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H Medium Broth |
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| DJ1019 | Bio Basic | 100g | EUR 60.88 |
Agarose, medium EEO |
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| GE4750-100G | Glentham Life Sciences | 100 g | EUR 166 |
Expansion of mouse primitive hematopoietic cells in three-dimensional cultures on chemically fixed stromal cell layers
To establish a practical and convenient method to expand hematopoietic cells (HCs), we applied chemically-fixed stromal cell layers formed within three-dimensional (3D) scaffolds to feeder of HC cultures. The HCs were expanded using two successive cultures. First, stromal cells were cultured within porous polymer scaffolds and formed tissue-engineered constructs (TECs); the scaffolds containing stromal cells, were fixed using aldehyde (formaldehyde or glutaraldehyde) or organic solvents (acetone, methanol or ethanol).
Second, mouse fetal liver cells (FLCs), as a source of HCs, were cultured on the TECs for 2 weeks, and the effects of fixative solutions on expansion of primitive HCs (c-kit+ and CD34+ cells) were examined. In the cultures on aldehyde-fixed TECs, primitive HCs were expanded 2.5- to 5.1-fold in the cultures on TECs fixed with glutaraldehyde, whereas no expansions were detected in those fixed with formaldehyde.
However, we achieved expansion of primitive HCs > fivefold in the cultures using TECs fixed with organic solvents. Among these solvents, the highest expansions-of roughly tenfold-were obtained using acetone fixation. Ethanol-fixed TECs also supported the expansion of the primitive HCs well (6.6- to 8.0-fold). In addition to these sufficient expansions, the procedure and storage of fixed TECs is fairly easy. Thus, HC expansion on chemically-fixed TECs may be a practical method for expanding primitive HCs.
Exploiting the potential of commercial digital holographic microscopy by combining it with 3D matrix cellculture assays
3D cell culture assays are becoming increasingly popular due to their higher resemblance to tissue environment. These provide an increased complexity compared to the growth on 2D surface and therefore allow studies of advanced cellular properties such as invasion.
We report here on the use of 3D Matrigel cell preparations combined with a particular gentle and informative type of live-cell microscopy: quantitative digital holographic microscopy (DHM), here performed by a commercial software-integrated system, currently mostly used for 2D cell culture preparations. By demonstrating this compatibility, we highlight the possible time-efficient quantitative analysis obtained by using a commercial software-integrated DHM system, also for cells in a more advanced 3D culture environment.
Further, we demonstrate two very different examples making use of this advantage by performing quantitative DHM analysis of: (1) wound closure cell monolayer Matrigel invasion assay and (2) Matrigel-trapped single and clumps of suspension cells. For both these, we benefited from the autofocus functionality of digital phase holographic imaging to obtain 3D information for cells migrating in a 3D environment. For the latter, we demonstrate that it is possible to quantitatively measure tumourigenic properties like growth of cell clump (or spheroid) over time, as well as single-cell invasion out of cell clump and into the surrounding extracellular matrix.
Overall, our findings highlight several possibilities for 3D digital holographic microscopy applications combined with 3D cell preparations, therein studies of drug response or genetic alterations on invasion capacity as well as on tumour growth and metastasis.
OryzaExp? IGF-1 LR3, Human Recombinant |
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| P1399-1 | Biovision | EUR 881 | |
1 mm Preparative Comb, RM |
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| 2328934 | Atto | 3unit | EUR 253 |
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Description: 1 sampl e wel l and 1 r efer ence wel l |
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1 mm Blank Comb, RM |
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| 2399041 | Atto | 4unit | EUR 272 |
rM IL-2 |
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| AK8323-0005 | Akron Biotech | 5µg | Ask for price |
rM IL-2 |
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| AK8323-0020 | Akron Biotech | 20µg | Ask for price |
rM IL-2 |
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| AK8323-0100 | Akron Biotech | 100µg | Ask for price |
rM IL-2 |
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| AK8323-1000 | Akron Biotech | 1mg | Ask for price |
IGF-II, human recombinant |
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| P1017-1 | ApexBio | 1 mg | EUR 1998 |
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Description: Insulin-like Growth Factor-II (IGF-II) is a polypeptide endocrine hormone structurally similar to insulin and belongs to insulin-like growth factor family. |
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1 mm 6-well Comb, RM |
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| 2328375 | Atto | 3unit | EUR 255 |
1 mm 8-well Comb, RM |
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| 2328376 | Atto | 3unit | EUR 255 |
1 mm 20-well Comb, RM |
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| 2328422 | Atto | 3unit | EUR 311 |
1 mm 10-well Comb, RM |
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| 2328948 | Atto | 3unit | EUR 288 |
1 mm Plate Set, RM-10 |
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| 2398214 | Atto | 3unit | EUR 281 |
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Description: 1 pai r of gl ass pl at es + 1 gask et , for 1 mm gel |
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1 mm Gaskets 3/pk, RM |
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| 2398237 | Atto | 5unit | EUR 257 |
Recombinant Human IGF-II Protein |
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| PROTP01344-1 | BosterBio | 50ug | EUR 317 |
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Description: The IGFs are mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. IGFs are predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGFs belong to the Insulin gene family, which also contains insulin and relaxin. The IGFs are similar by structure and function to insulin, but have a much higher growth-promoting activity than insulin. IGF-II expression is influenced by placenta lactogen, while IGF-I expression is regulated by growth hormone. Both IGF-I and IGF-II signal through the tyrosine kinase type I receptor (IGF-IR), but, IGF-II can also signal through the IGF-II/Mannose-6-phosphate receptor. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions. Recombinant human IGF-I and IGF-II are globular proteins containing 70 and 67 amino acids, respectively, and 3 intra-molecular disulfide bonds. |
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Recombinant Murine IGF-I Protein |
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| PROTP05017-1 | BosterBio | 50ug | EUR 317 |
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Description: The IGFs are mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. IGFs are predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGFs belong to the Insulin gene family, which also contains insulin and relaxin. The IGFs are similar by structure and function to insulin, but have a much higher growth-promoting activity than insulin. IGF-II expression is influenced by placenta lactogen, while IGF-I expression is regulated by growth hormone. Both IGF-I and IGF-II signal through the tyrosine kinase type I receptor (IGF-IR), but, IGF-II can also signal through the IGF-II/Mannose-6-phosphate receptor. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions. Recombinant murine IGF-I is a 7.6 kDa globular protein containing amino acids including 3 intra-molecular disulfide bonds. |
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Recombinant Human IGF-I Protein |
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| PROTP05019-1 | BosterBio | 500ug | EUR 317 |
|
Description: The IGFs are mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. IGFs are predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGFs belong to the Insulin gene family, which also contains insulin and relaxin. The IGFs are similar by structure and function to insulin, but have a much higher growth-promoting activity than insulin. IGF-II expression is influenced by placenta lactogen, while IGF-I expression is regulated by growth hormone. Both IGF-I and IGF-II signal through the tyrosine kinase type I receptor (IGF-IR), but, IGF-II can also signal through the IGF-II/Mannose-6-phosphate receptor. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions. Recombinant human IGF-I and IGF-II are globular proteins containing 70 and 67 amino acids, respectively, and 3 intra-molecular disulfide bonds. |
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Recombinant Human IGF-BP1 Protein |
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| PROTP08833-1 | BosterBio | 25ug | EUR 317 |
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Description: IGF-BPs controls the distribution, function and activity of IGFs in various cell tissues and body fluids. Currently there are seven named IGF-BPs that form high affinity complexes with both IGF-I and IGF-II. IGF-BP1 is a 25.4 kDa cysteine-rich secreted protein expressed in liver, deciduas, and kidneys and is the most abundant IGF-BP in amniotic fluid. Levels of IGF-BP1 in serum are lowest after food. IGF-BP1 binds to both IGF-I and IGF-II with equal affinity. Phosphorylated IGF-BP1 hinders IGF actions, where as nonphosphorylated IGF-BP1 is stimulatory. Recombinant human IGF-BP1 is a 25.4 kDa protein consisting of 235 amino acid residues (Isoform A). |
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Recombinant Human IGF-BP4 Protein |
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| PROTP22692-1 | BosterBio | 20ug | EUR 317 |
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Description: IGF-BPs control the distribution, function and activity of IGFs in various cell tissues and body fluids. IGF-BP4 is the major IGF-BP produced by osteoblasts, and is also found in the epidermis, ovarian follicles, and other tissues. IGF-BP4 inhibits the activity of IGF-I and IGF-II by binding in a manner that results in the formation of complexes with reduced ability to signal through cell surface IGF receptors. IGF-BP4 can inhibit the growth of chick pelvis cartilage and HT29 colon adenocarcinoma cells by blocking the mitogenic actions of IGFs, and has also been shown to reduce colony formation by colorectal cancer cells via an IGF independent pathway. The biological effects of IGF-BP4 can be regulated by Pregnancy Associated Plasma Protein A (PAPP-A), which reduces IGF-BP4/IGF binding affinity by proteolytically cleaving IGF-BP4. The modulation of IGF-BP4 activity by PAPP-A is an important component in the regulation of ovarian folliculogenesis and in the growth inhibition of responding ovarian cancer cells. Recombinant human IGF-BP4 is a 25.8 kDa protein consisting of 237 amino acid residues including the IGF-BP domain and thyroglobulin type-I domain. *Manufactured using (BTI-Tn-5B1-4) cells under license from the Boyce Thompson Institute for Plant Research, Inc. |
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Recombinant Human IGF-BP5 Protein |
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| PROTP24593-1 | BosterBio | 25ug | EUR 317 |
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Description: IGF-BPs controls the distribution, function and activity of IGFs in various cell tissues and body fluids. Currently there are seven named IGF-BPs that form high affinity complexes with both IGF-I and IGF-II. IGF-BP5 is a 28.6 kDa cysteine-rich secreted protein produced by vascular smooth muscle cells. It is the major IGF-binding protein present in bone tissue and helps potentiate the action of IGF-I on smooth muscle cells, fibroblasts or osteoblasts. Data shows that IGFBP-5 acts as a growth inhibitor and pro-apoptotic agent in breast cancer cells. IGFBP-5 overexpressing mice show an increase in neonatal mortality, reduced female fertility, whole-body growth inhibition and retarded muscle development. Recombinant human IGF-BP5 is a 28.6 kDa protein consisting of 253 amino acid residues. |
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Human Insulin-like Growth Factor 1 (IGF-1) AssayMax ELISA Kit |
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| EI1001-1 | AssayPro | 96 Well Plate | EUR 477 |
Mouse Insulin-like Growth Factor 1 (IGF-1) AssayMax ELISA Kit |
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| EMI1001-1 | AssayPro | 96 Well Plate | EUR 477 |
Clips 4/pk RM |
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| 2398239 | Atto | 6unit | EUR 250 |
1mm Pressure Platen - RM |
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| 2398242 | Atto | 3unit | EUR 281 |
Anti-IGF-1R (Ab-1161) Antibody |
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| A00070-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal IGF-1R (Ab-1161) Antibody. Validated in IF, IHC, WB and tested in Human, Mouse, Rat. |
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IGF-1, human |
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| RC216-12 | Bio Basic | 1mg | EUR 376.25 |
IGF-1, rat |
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| RC256-12 | Bio Basic | 50ug | EUR 152.66 |
IGF-1, Human |
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| HY-P7018 | MedChemExpress | 10ug | EUR 108 |
IGF-1, Mouse |
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| HY-P7070 | MedChemExpress | 100ug | EUR 452 |
LR3-IGF-1 |
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| E21-032 | EnoGene | 10ug | EUR 343 |
IGF-1-Fc |
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| E13-m003-2 | EnoGene | 50μg | EUR 830 |
IGF-1 Protein |
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| abx060289-100ug | Abbexa | 100 ug | EUR 411 |
IGF- 1 Antibody |
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| ABD6096 | Lifescience Market | 100 ug | EUR 438 |
IGF-1 Antibody |
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| DF6096 | Affbiotech | 200ul | EUR 304 |
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Description: IGF-1 Antibody detects endogenous levels of total IGF-1. |
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rHu IGF-1 |
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| AK8530-0020 | Akron Biotech | 20µg | Ask for price |
rHu IGF-1 |
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| AK8530-0100 | Akron Biotech | 100µg | Ask for price |
rHu IGF-1 |
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| AK8530-1000 | Akron Biotech | 1mg | Ask for price |
IGF- 1 Plasmid |
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| PVT7112 | Lifescience Market | 2 ug | EUR 266 |
0.75mm Plate Set,RM-075 |
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| 2393014 | Atto | 2unit | EUR 263 |
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Description: 1 pai r of gl ass pl at es + 1 gask et , for 0.75 mm gel |
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5 mm Dummy Plate, RM |
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| 2393074 | Atto | 10unit | EUR 241 |
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Description: Pl ast i c pl at e for r unni ng 1 Pagel pr ecast gel onl y |
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2mm Plate Set,RM-22 |
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| 2393740 | Atto | 3unit | EUR 281 |
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Description: 1 pai r of gl ass pl at es + 1 gask et , for 2 mm gel |
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7 mm Dummy Plate, RM |
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| 2398247 | Atto | 10unit | EUR 241 |
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Description: Pl ast i c pl at e for r unni ng 1 hand-cast gel onl y |
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1 mm 21-well Combs 2/pk, RM |
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| 2398255 | Atto | 5unit | EUR 272 |
IGF-1 ELISA Kit |
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| DEIA1901 | Creative Diagnostics | 96T | EUR 757 |
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Description: The IGF-1 ELISA is an enzyme immunoassay for the quantitative in vitro diagnostic measurement of Insulin-like Growth Factor 1 (IGF-1) in serum. |
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Mouse IGF-1 Antibody |
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| 33079-05111 | AssayPro | 150 ug | EUR 261 |
IGF-I (1-3) |
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| 5-01367 | CHI Scientific | 4 x 5mg | Ask for price |
IGF-1(M23) Antibody |
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| BNC040220-100 | Biotium | 100uL | EUR 199 |
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Description: Primary antibody against IGF-1(M23), CF405S conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNC040220-500 | Biotium | 500uL | EUR 544 |
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Description: Primary antibody against IGF-1(M23), CF405S conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNC470220-100 | Biotium | 100uL | EUR 199 |
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Description: Primary antibody against IGF-1(M23), CF647 conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNC470220-500 | Biotium | 500uL | EUR 544 |
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Description: Primary antibody against IGF-1(M23), CF647 conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNC550220-100 | Biotium | 100uL | EUR 199 |
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Description: Primary antibody against IGF-1(M23), CF555 conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNC550220-500 | Biotium | 500uL | EUR 544 |
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Description: Primary antibody against IGF-1(M23), CF555 conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNCB0220-100 | Biotium | 100uL | EUR 199 |
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Description: Primary antibody against IGF-1(M23), Biotin conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNCB0220-500 | Biotium | 500uL | EUR 544 |
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Description: Primary antibody against IGF-1(M23), Biotin conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNC810220-100 | Biotium | 100uL | EUR 199 |
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Description: Primary antibody against IGF-1(M23), CF680R conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNC810220-500 | Biotium | 500uL | EUR 544 |
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Description: Primary antibody against IGF-1(M23), CF680R conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNC700220-100 | Biotium | 100uL | EUR 199 |
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Description: Primary antibody against IGF-1(M23), CF770 conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNC700220-500 | Biotium | 500uL | EUR 544 |
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Description: Primary antibody against IGF-1(M23), CF770 conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNC880220-100 | Biotium | 100uL | EUR 199 |
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Description: Primary antibody against IGF-1(M23), CF488A conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNC880220-500 | Biotium | 500uL | EUR 544 |
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Description: Primary antibody against IGF-1(M23), CF488A conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNC940220-100 | Biotium | 100uL | EUR 199 |
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Description: Primary antibody against IGF-1(M23), CF594 conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNC940220-500 | Biotium | 500uL | EUR 544 |
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Description: Primary antibody against IGF-1(M23), CF594 conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNC680220-100 | Biotium | 100uL | EUR 199 |
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Description: Primary antibody against IGF-1(M23), CF568 conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNC680220-500 | Biotium | 500uL | EUR 544 |
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Description: Primary antibody against IGF-1(M23), CF568 conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNCA0220-250 | Biotium | 250uL | EUR 383 |
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Description: Primary antibody against IGF-1(M23), APC conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNCAP0220-100 | Biotium | 100uL | EUR 199 |
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Description: Primary antibody against IGF-1(M23), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNCAP0220-500 | Biotium | 500uL | EUR 544 |
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Description: Primary antibody against IGF-1(M23), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNUM0220-50 | Biotium | 50uL | EUR 395 |
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Description: Primary antibody against IGF-1(M23), 1mg/mL |
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IGF-1(M23) Antibody |
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| BNCP0220-250 | Biotium | 250uL | EUR 383 |
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Description: Primary antibody against IGF-1(M23), PerCP conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNC050220-100 | Biotium | 100uL | EUR 199 |
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Description: Primary antibody against IGF-1(M23), CF405M conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNC050220-500 | Biotium | 500uL | EUR 544 |
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Description: Primary antibody against IGF-1(M23), CF405M conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNC400220-100 | Biotium | 100uL | EUR 199 |
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Description: Primary antibody against IGF-1(M23), CF640R conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNC400220-500 | Biotium | 500uL | EUR 544 |
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Description: Primary antibody against IGF-1(M23), CF640R conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNCR0220-250 | Biotium | 250uL | EUR 383 |
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Description: Primary antibody against IGF-1(M23), RPE conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNUB0220-100 | Biotium | 100uL | EUR 209 |
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Description: Primary antibody against IGF-1(M23), Concentration: 0.2mg/mL |
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IGF-1(M23) Antibody |
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| BNUB0220-500 | Biotium | 500uL | EUR 458 |
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Description: Primary antibody against IGF-1(M23), Concentration: 0.2mg/mL |
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IGF-1(M23) Antibody |
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| BNC430220-100 | Biotium | 100uL | EUR 199 |
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Description: Primary antibody against IGF-1(M23), CF543 conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNC430220-500 | Biotium | 500uL | EUR 544 |
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Description: Primary antibody against IGF-1(M23), CF543 conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNCH0220-100 | Biotium | 100uL | EUR 199 |
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Description: Primary antibody against IGF-1(M23), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNCH0220-500 | Biotium | 500uL | EUR 544 |
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Description: Primary antibody against IGF-1(M23), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNC610220-100 | Biotium | 100uL | EUR 199 |
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Description: Primary antibody against IGF-1(M23), CF660R conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNC610220-500 | Biotium | 500uL | EUR 544 |
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Description: Primary antibody against IGF-1(M23), CF660R conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNC800220-100 | Biotium | 100uL | EUR 199 |
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Description: Primary antibody against IGF-1(M23), CF680 conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNC800220-500 | Biotium | 500uL | EUR 544 |
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Description: Primary antibody against IGF-1(M23), CF680 conjugate, Concentration: 0.1mg/mL |
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Human IGF-1 LR3 |
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| SP-89927-100 | Alpha Diagnostics | 100 ug | EUR 225 |
IGF-1, human recombinant |
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| P1016-.02 | ApexBio | 20 µg | EUR 258 |
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Description: Insulin-like growth factor I (IGF-1) is a polypeptide endocrine hormone structurally similar to insulin and is mainly produced in the liver when stimulated by growth hormone. IGF-1 is a growth factor that stimulates the proliferation of various cell types including muscle, bone, and cartilage tissue |
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IGF-I (1-3) |
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| H-2468.0250 | Bachem | 250.0mg | EUR 176 |
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Description: Sum Formula: C12H19N3O6; CAS# [32302-76-4] |
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IGF-I (1-3) |
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| H-2468.1000 | Bachem | 1.0g | EUR 466 |
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Description: Sum Formula: C12H19N3O6; CAS# [32302-76-4] |
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IGF-1 Blocking Peptide |
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| DF6096-BP | Affbiotech | 1mg | EUR 195 |
rHu IGF-1 (cGMP) |
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| AK9901-0000 | Akron Biotech | 1mg | Ask for price |
A-M310 agarGEL RM, 10/pk |
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| 2332200 | Atto | 2unit | EUR 351 |
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Description: pH 3 - 10 |
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A-M38 agarGEL RM, 10/pk |
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| 2332210 | Atto | 2unit | EUR 351 |
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Description: pH 3 - 8 |
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A-M58 agarGEL RM, 10/pk |
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| 2332220 | Atto | 2unit | EUR 351 |
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Description: pH 5 - 8 |
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A-M510 agarGEL RM, 10/pk |
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| 2332230 | Atto | 2unit | EUR 351 |
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Description: pH 5 - 10 |
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