Development of a bioreactor-system for pre-endothelialized cardiac patch generation with enhanced viscoelastic properties by combined collagen I compression and stromal cell culture
- Treatment of terminal heart failure still poses a significant clinical problem. Cardiac tissue engineering could offer autologous solutions for the replacement of non-functional myocardial tissue. So far, soft matrix construction and missing large scale prevascularization prevented the application of sizeable cardiac repair patches. We developed a novel bioreactor system for semi-automatic compression of a collagen I hydrogel applying 16 times higher pressure than in previous studies.
- Resistance towards compression stress was investigated for multiple cardiac-related cell types. For scaffold prevascuarization, a tubular cavity was imprinted during the compaction process. Primary cardiac-derived endothelial cells (EC) were isolated from human left atrial appendages (HLAA) and characterized by FACS and immunocytology. EC were then seeded into the preformed channel with dermal fibroblasts as interstitial cell component of the fully cellularized patch.
- After eight days of constant perfusion culture within the same bioreactor, scaffold dynamic modulus and cell viability were analyzed. Endothelial proliferation and vessel maturation were examined by immunohistochemistry and transmission electron microscopy. Our design allowed for scaffold production and dynamic culture in a one-stop-shop model.
- Enhanced compression and cell-mediated matrix remodeling induced a significant increase in scaffold stiffness while ensuring excellent cell survival. For the first time, we could isolate HLAA-derived EC with proliferative potential.
- Endothelial cells within the central channel proliferated during flow culture, continuously expressing endothelial markers (CD31) and displaying basal membrane synthesis (collagen IV, ultrastructural analysis). After seven days of culture, a complete endothelial monolayer could be observed. Covering cells aligned themselves in flow direction and developed mature cell-cell-contacts.
pharmacodane
1 mm Blank Comb, RM |
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| 2399041 | Atto | 4unit | EUR 272 |
rM IL-2 |
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| AK8323-0005 | Akron Biotech | 5µg | Ask for price |
rM IL-2 |
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| AK8323-0020 | Akron Biotech | 20µg | Ask for price |
rM IL-2 |
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| AK8323-0100 | Akron Biotech | 100µg | Ask for price |
rM IL-2 |
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| AK8323-1000 | Akron Biotech | 1mg | Ask for price |
IGF-II, human recombinant |
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| P1017-1 | ApexBio | 1 mg | EUR 1998 |
|
Description: Insulin-like Growth Factor-II (IGF-II) is a polypeptide endocrine hormone structurally similar to insulin and belongs to insulin-like growth factor family. |
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1 mm 6-well Comb, RM |
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| 2328375 | Atto | 3unit | EUR 255 |
1 mm 8-well Comb, RM |
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| 2328376 | Atto | 3unit | EUR 255 |
1 mm 20-well Comb, RM |
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| 2328422 | Atto | 3unit | EUR 311 |
1 mm 10-well Comb, RM |
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| 2328948 | Atto | 3unit | EUR 288 |
1 mm Plate Set, RM-10 |
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| 2398214 | Atto | 3unit | EUR 281 |
|
Description: 1 pai r of gl ass pl at es + 1 gask et , for 1 mm gel |
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1 mm Gaskets 3/pk, RM |
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| 2398237 | Atto | 5unit | EUR 257 |
Recombinant Human IGF-II Protein |
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| PROTP01344-1 | BosterBio | 50ug | EUR 317 |
|
Description: The IGFs are mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. IGFs are predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGFs belong to the Insulin gene family, which also contains insulin and relaxin. The IGFs are similar by structure and function to insulin, but have a much higher growth-promoting activity than insulin. IGF-II expression is influenced by placenta lactogen, while IGF-I expression is regulated by growth hormone. Both IGF-I and IGF-II signal through the tyrosine kinase type I receptor (IGF-IR), but, IGF-II can also signal through the IGF-II/Mannose-6-phosphate receptor. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions. Recombinant human IGF-I and IGF-II are globular proteins containing 70 and 67 amino acids, respectively, and 3 intra-molecular disulfide bonds. |
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Recombinant Murine IGF-I Protein |
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| PROTP05017-1 | BosterBio | 50ug | EUR 317 |
|
Description: The IGFs are mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. IGFs are predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGFs belong to the Insulin gene family, which also contains insulin and relaxin. The IGFs are similar by structure and function to insulin, but have a much higher growth-promoting activity than insulin. IGF-II expression is influenced by placenta lactogen, while IGF-I expression is regulated by growth hormone. Both IGF-I and IGF-II signal through the tyrosine kinase type I receptor (IGF-IR), but, IGF-II can also signal through the IGF-II/Mannose-6-phosphate receptor. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions. Recombinant murine IGF-I is a 7.6 kDa globular protein containing amino acids including 3 intra-molecular disulfide bonds. |
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Recombinant Human IGF-I Protein |
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| PROTP05019-1 | BosterBio | 500ug | EUR 317 |
|
Description: The IGFs are mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. IGFs are predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGFs belong to the Insulin gene family, which also contains insulin and relaxin. The IGFs are similar by structure and function to insulin, but have a much higher growth-promoting activity than insulin. IGF-II expression is influenced by placenta lactogen, while IGF-I expression is regulated by growth hormone. Both IGF-I and IGF-II signal through the tyrosine kinase type I receptor (IGF-IR), but, IGF-II can also signal through the IGF-II/Mannose-6-phosphate receptor. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions. Recombinant human IGF-I and IGF-II are globular proteins containing 70 and 67 amino acids, respectively, and 3 intra-molecular disulfide bonds. |
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Recombinant Human IGF-BP1 Protein |
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| PROTP08833-1 | BosterBio | 25ug | EUR 317 |
|
Description: IGF-BPs controls the distribution, function and activity of IGFs in various cell tissues and body fluids. Currently there are seven named IGF-BPs that form high affinity complexes with both IGF-I and IGF-II. IGF-BP1 is a 25.4 kDa cysteine-rich secreted protein expressed in liver, deciduas, and kidneys and is the most abundant IGF-BP in amniotic fluid. Levels of IGF-BP1 in serum are lowest after food. IGF-BP1 binds to both IGF-I and IGF-II with equal affinity. Phosphorylated IGF-BP1 hinders IGF actions, where as nonphosphorylated IGF-BP1 is stimulatory. Recombinant human IGF-BP1 is a 25.4 kDa protein consisting of 235 amino acid residues (Isoform A). |
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Recombinant Human IGF-BP4 Protein |
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| PROTP22692-1 | BosterBio | 20ug | EUR 317 |
|
Description: IGF-BPs control the distribution, function and activity of IGFs in various cell tissues and body fluids. IGF-BP4 is the major IGF-BP produced by osteoblasts, and is also found in the epidermis, ovarian follicles, and other tissues. IGF-BP4 inhibits the activity of IGF-I and IGF-II by binding in a manner that results in the formation of complexes with reduced ability to signal through cell surface IGF receptors. IGF-BP4 can inhibit the growth of chick pelvis cartilage and HT29 colon adenocarcinoma cells by blocking the mitogenic actions of IGFs, and has also been shown to reduce colony formation by colorectal cancer cells via an IGF independent pathway. The biological effects of IGF-BP4 can be regulated by Pregnancy Associated Plasma Protein A (PAPP-A), which reduces IGF-BP4/IGF binding affinity by proteolytically cleaving IGF-BP4. The modulation of IGF-BP4 activity by PAPP-A is an important component in the regulation of ovarian folliculogenesis and in the growth inhibition of responding ovarian cancer cells. Recombinant human IGF-BP4 is a 25.8 kDa protein consisting of 237 amino acid residues including the IGF-BP domain and thyroglobulin type-I domain. *Manufactured using (BTI-Tn-5B1-4) cells under license from the Boyce Thompson Institute for Plant Research, Inc. |
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Recombinant Human IGF-BP5 Protein |
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| PROTP24593-1 | BosterBio | 25ug | EUR 317 |
|
Description: IGF-BPs controls the distribution, function and activity of IGFs in various cell tissues and body fluids. Currently there are seven named IGF-BPs that form high affinity complexes with both IGF-I and IGF-II. IGF-BP5 is a 28.6 kDa cysteine-rich secreted protein produced by vascular smooth muscle cells. It is the major IGF-binding protein present in bone tissue and helps potentiate the action of IGF-I on smooth muscle cells, fibroblasts or osteoblasts. Data shows that IGFBP-5 acts as a growth inhibitor and pro-apoptotic agent in breast cancer cells. IGFBP-5 overexpressing mice show an increase in neonatal mortality, reduced female fertility, whole-body growth inhibition and retarded muscle development. Recombinant human IGF-BP5 is a 28.6 kDa protein consisting of 253 amino acid residues. |
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Human Insulin-like Growth Factor 1 (IGF-1) AssayMax ELISA Kit |
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| EI1001-1 | AssayPro | 96 Well Plate | EUR 477 |
Mouse Insulin-like Growth Factor 1 (IGF-1) AssayMax ELISA Kit |
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| EMI1001-1 | AssayPro | 96 Well Plate | EUR 477 |
Clips 4/pk RM |
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| 2398239 | Atto | 6unit | EUR 250 |
1mm Pressure Platen - RM |
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| 2398242 | Atto | 3unit | EUR 281 |
Anti-IGF-1R (Ab-1161) Antibody |
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| A00070-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal IGF-1R (Ab-1161) Antibody. Validated in IF, IHC, WB and tested in Human, Mouse, Rat. |
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IGF-1, Human |
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| HY-P7018 | MedChemExpress | 10ug | EUR 108 |
IGF-1, Mouse |
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| HY-P7070 | MedChemExpress | 100ug | EUR 452 |
IGF-1-Fc |
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| E13-m003-2 | EnoGene | 50μg | EUR 830 |
LR3-IGF-1 |
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| E21-032 | EnoGene | 10ug | EUR 343 |
IGF-1 Protein |
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| abx060289-100ug | Abbexa | 100 ug | EUR 411 |
IGF-1, human |
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| RC216-12 | Bio Basic | 1mg | EUR 376.25 |
IGF-1, rat |
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| RC256-12 | Bio Basic | 50ug | EUR 152.66 |
rHu IGF-1 |
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| AK8530-0020 | Akron Biotech | 20µg | Ask for price |
rHu IGF-1 |
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| AK8530-0100 | Akron Biotech | 100µg | Ask for price |
rHu IGF-1 |
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| AK8530-1000 | Akron Biotech | 1mg | Ask for price |
IGF-1 Antibody |
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| DF6096 | Affbiotech | 200ul | EUR 304 |
|
Description: IGF-1 Antibody detects endogenous levels of total IGF-1. |
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IGF- 1 Antibody |
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| ABD6096 | Lifescience Market | 100 ug | EUR 438 |
IGF- 1 Plasmid |
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| PVT7112 | Lifescience Market | 2 ug | EUR 266 |
0.75mm Plate Set,RM-075 |
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| 2393014 | Atto | 2unit | EUR 263 |
|
Description: 1 pai r of gl ass pl at es + 1 gask et , for 0.75 mm gel |
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5 mm Dummy Plate, RM |
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| 2393074 | Atto | 10unit | EUR 241 |
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Description: Pl ast i c pl at e for r unni ng 1 Pagel pr ecast gel onl y |
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2mm Plate Set,RM-22 |
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| 2393740 | Atto | 3unit | EUR 281 |
|
Description: 1 pai r of gl ass pl at es + 1 gask et , for 2 mm gel |
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7 mm Dummy Plate, RM |
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| 2398247 | Atto | 10unit | EUR 241 |
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Description: Pl ast i c pl at e for r unni ng 1 hand-cast gel onl y |
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1 mm 21-well Combs 2/pk, RM |
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| 2398255 | Atto | 5unit | EUR 272 |
IGF-1 ELISA Kit |
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| DEIA1901 | Creative Diagnostics | 96T | EUR 757 |
|
Description: The IGF-1 ELISA is an enzyme immunoassay for the quantitative in vitro diagnostic measurement of Insulin-like Growth Factor 1 (IGF-1) in serum. |
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IGF-1(M23) Antibody |
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| BNCA0220-250 | Biotium | 250uL | EUR 383 |
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Description: Primary antibody against IGF-1(M23), APC conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNCAP0220-100 | Biotium | 100uL | EUR 199 |
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Description: Primary antibody against IGF-1(M23), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNCAP0220-500 | Biotium | 500uL | EUR 544 |
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Description: Primary antibody against IGF-1(M23), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNC810220-100 | Biotium | 100uL | EUR 199 |
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Description: Primary antibody against IGF-1(M23), CF680R conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNC810220-500 | Biotium | 500uL | EUR 544 |
|
Description: Primary antibody against IGF-1(M23), CF680R conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNC880220-100 | Biotium | 100uL | EUR 199 |
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Description: Primary antibody against IGF-1(M23), CF488A conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNC880220-500 | Biotium | 500uL | EUR 544 |
|
Description: Primary antibody against IGF-1(M23), CF488A conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNCB0220-100 | Biotium | 100uL | EUR 199 |
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Description: Primary antibody against IGF-1(M23), Biotin conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNCB0220-500 | Biotium | 500uL | EUR 544 |
|
Description: Primary antibody against IGF-1(M23), Biotin conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNC550220-100 | Biotium | 100uL | EUR 199 |
|
Description: Primary antibody against IGF-1(M23), CF555 conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNC550220-500 | Biotium | 500uL | EUR 544 |
|
Description: Primary antibody against IGF-1(M23), CF555 conjugate, Concentration: 0.1mg/mL |
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IGF-I (1-3) |
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| 5-01367 | CHI Scientific | 4 x 5mg | Ask for price |
IGF-1(M23) Antibody |
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| BNC470220-100 | Biotium | 100uL | EUR 199 |
|
Description: Primary antibody against IGF-1(M23), CF647 conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNC470220-500 | Biotium | 500uL | EUR 544 |
|
Description: Primary antibody against IGF-1(M23), CF647 conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNC940220-100 | Biotium | 100uL | EUR 199 |
|
Description: Primary antibody against IGF-1(M23), CF594 conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNC940220-500 | Biotium | 500uL | EUR 544 |
|
Description: Primary antibody against IGF-1(M23), CF594 conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNCP0220-250 | Biotium | 250uL | EUR 383 |
|
Description: Primary antibody against IGF-1(M23), PerCP conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNC040220-100 | Biotium | 100uL | EUR 199 |
|
Description: Primary antibody against IGF-1(M23), CF405S conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNC040220-500 | Biotium | 500uL | EUR 544 |
|
Description: Primary antibody against IGF-1(M23), CF405S conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNC050220-100 | Biotium | 100uL | EUR 199 |
|
Description: Primary antibody against IGF-1(M23), CF405M conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNC050220-500 | Biotium | 500uL | EUR 544 |
|
Description: Primary antibody against IGF-1(M23), CF405M conjugate, Concentration: 0.1mg/mL |
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Mouse IGF-1 Antibody |
|||
| 33079-05111 | AssayPro | 150 ug | EUR 261 |
IGF-1(M23) Antibody |
|||
| BNCR0220-250 | Biotium | 250uL | EUR 383 |
|
Description: Primary antibody against IGF-1(M23), RPE conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNUB0220-100 | Biotium | 100uL | EUR 209 |
|
Description: Primary antibody against IGF-1(M23), Concentration: 0.2mg/mL |
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IGF-1(M23) Antibody |
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| BNUB0220-500 | Biotium | 500uL | EUR 458 |
|
Description: Primary antibody against IGF-1(M23), Concentration: 0.2mg/mL |
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IGF-1(M23) Antibody |
|||
| BNC680220-100 | Biotium | 100uL | EUR 199 |
|
Description: Primary antibody against IGF-1(M23), CF568 conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
|||
| BNC680220-500 | Biotium | 500uL | EUR 544 |
|
Description: Primary antibody against IGF-1(M23), CF568 conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
|||
| BNC700220-100 | Biotium | 100uL | EUR 199 |
|
Description: Primary antibody against IGF-1(M23), CF770 conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
|||
| BNC700220-500 | Biotium | 500uL | EUR 544 |
|
Description: Primary antibody against IGF-1(M23), CF770 conjugate, Concentration: 0.1mg/mL |
|||
IGF-1(M23) Antibody |
|||
| BNC800220-100 | Biotium | 100uL | EUR 199 |
|
Description: Primary antibody against IGF-1(M23), CF680 conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
|||
| BNC800220-500 | Biotium | 500uL | EUR 544 |
|
Description: Primary antibody against IGF-1(M23), CF680 conjugate, Concentration: 0.1mg/mL |
|||
IGF-1(M23) Antibody |
|||
| BNC400220-100 | Biotium | 100uL | EUR 199 |
|
Description: Primary antibody against IGF-1(M23), CF640R conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
|||
| BNC400220-500 | Biotium | 500uL | EUR 544 |
|
Description: Primary antibody against IGF-1(M23), CF640R conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
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| BNC610220-100 | Biotium | 100uL | EUR 199 |
|
Description: Primary antibody against IGF-1(M23), CF660R conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
|||
| BNC610220-500 | Biotium | 500uL | EUR 544 |
|
Description: Primary antibody against IGF-1(M23), CF660R conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
|||
| BNC430220-100 | Biotium | 100uL | EUR 199 |
|
Description: Primary antibody against IGF-1(M23), CF543 conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
|||
| BNC430220-500 | Biotium | 500uL | EUR 544 |
|
Description: Primary antibody against IGF-1(M23), CF543 conjugate, Concentration: 0.1mg/mL |
|||
IGF-1(M23) Antibody |
|||
| BNCH0220-100 | Biotium | 100uL | EUR 199 |
|
Description: Primary antibody against IGF-1(M23), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL |
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IGF-1(M23) Antibody |
|||
| BNCH0220-500 | Biotium | 500uL | EUR 544 |
|
Description: Primary antibody against IGF-1(M23), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL |
|||
IGF-1(M23) Antibody |
|||
| BNUM0220-50 | Biotium | 50uL | EUR 395 |
|
Description: Primary antibody against IGF-1(M23), 1mg/mL |
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IGF-1, human recombinant |
|||
| P1016-.02 | ApexBio | 20 µg | EUR 258 |
|
Description: Insulin-like growth factor I (IGF-1) is a polypeptide endocrine hormone structurally similar to insulin and is mainly produced in the liver when stimulated by growth hormone. IGF-1 is a growth factor that stimulates the proliferation of various cell types including muscle, bone, and cartilage tissue |
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Human IGF-1 LR3 |
|||
| SP-89927-100 | Alpha Diagnostics | 100 ug | EUR 225 |
IGF-I (1-3) |
|||
| H-2468.0250 | Bachem | 250.0mg | EUR 176 |
|
Description: Sum Formula: C12H19N3O6; CAS# [32302-76-4] |
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IGF-I (1-3) |
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| H-2468.1000 | Bachem | 1.0g | EUR 466 |
|
Description: Sum Formula: C12H19N3O6; CAS# [32302-76-4] |
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rHu IGF-1 (cGMP) |
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| AK9901-0000 | Akron Biotech | 1mg | Ask for price |
IGF-1 Blocking Peptide |
|||
| DF6096-BP | Affbiotech | 1mg | EUR 195 |
A-M310 agarGEL RM, 10/pk |
|||
| 2332200 | Atto | 2unit | EUR 351 |
|
Description: pH 3 - 10 |
|||
A-M38 agarGEL RM, 10/pk |
|||
| 2332210 | Atto | 2unit | EUR 351 |
|
Description: pH 3 - 8 |
|||
A-M58 agarGEL RM, 10/pk |
|||
| 2332220 | Atto | 2unit | EUR 351 |
|
Description: pH 5 - 8 |
|||
A-M510 agarGEL RM, 10/pk |
|||
| 2332230 | Atto | 2unit | EUR 351 |
|
Description: pH 5 - 10 |
|||
Plant cell cultures as food-aspects of sustainability and safety
Sustainability and safety aspects of plant cell cultures as food are presented. Applicability of dairy side streams as carbon source and use of natural growth enhancers in cultivation are shown. Biotechnologically produced cellular products are currently emerging to replace and add into the portfolio of agriculturally derived commodities.
Plant cell cultures used for food could supplement current food production. However, still many aspects need to be resolved before this new food concept can enter the market. Issues related to sustainability and safety for human consumption are relevant for both consumers and regulators. In this study, two plant cell cultures, deriving from arctic bramble (Rubus arcticus) and birch (Betula pendula), were cultivated using lactose-rich dairy side streams as alternative carbon sources to replace sucrose.
Biomasses were comparable to those of original plant cell culture media when up to 83% and 75% of the original sucrose was replaced by these side streams for arctic bramble and birch cell cultures, respectively. Furthermore, nutritional composition or sensory properties were not compromised. Synthetic plant growth regulators were replaced by natural components, such as coconut water and IAA for several subculture cycles.
Finally, it was shown that only trace amounts of free growth regulators are present in the cells at the harvesting point and assessment by freshwater crustaceans assay indicated that toxicity of the cells was not exceeding that of traditionally consumed bilberry fruit.
Stability of live attenuated classical swine fever cellculture vaccine virus in liquid form for developing an oral vaccine
Classical swine fever (CSF) is an important viral disease of pigs and controlled by vaccination. Unorganised backyard and wild pigs are difficult to vaccinate by needle vaccination. Here we formulated liquid vaccines using an Indian CSF cell culture vaccine virus and four stabilisers and evaluated their stability at 4 °C, 25 °C and 37 °C up to 24 h for use as oral vaccine.
The stabilisers were Lactalbumin hydrolysate-Trehalose, Lactalbumin hydrolysate-Trehalose-Gelatin, Lactalbumin hydrolysate-Lactose-Sucrose and Lactalbumin hydrolysate-Sucrose. The liquid vaccines, with or without stabilisers, were stable at 4 °C up to 24 h, whereas, a drop of one log10 titre was observed at 25 °C during the same period. At 37 °C, the virus titre diminished by only one log10 with the Lactalbumin hydrolysate-Trehalose (LT) stabiliser up to 24 h compared to two log10 losses in virus titre with other stabilisers and virus control.
We therefore conclude that for developing a CSF oral vaccine, the vaccine virus in liquid form can be used directly during the winter, whereas for developing the oral vaccine for summer, the LT stabiliser would provide maximum stability to the virus to withstand the warm temperature while maintaining adequate therapeutic titre for inducing a protective immune response.
Mouse Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit |
|||
| DLR-EG-VEGF-Mu-48T | DL Develop | 48T | EUR 435 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
|||
Mouse Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit |
|||
| DLR-EG-VEGF-Mu-96T | DL Develop | 96T | EUR 561 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
|||
Rat Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit |
|||
| DLR-EG-VEGF-Ra-48T | DL Develop | 48T | EUR 454 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Rat Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
|||
Rat Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit |
|||
| DLR-EG-VEGF-Ra-96T | DL Develop | 96T | EUR 587 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Rat Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
|||
Bovine Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit |
|||
| RD-EG-VEGF-b-48Tests | Reddot Biotech | 48 Tests | EUR 494 |
Bovine Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit |
|||
| RD-EG-VEGF-b-96Tests | Reddot Biotech | 96 Tests | EUR 684 |
Human Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit |
|||
| RD-EG-VEGF-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 317 |
Human Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit |
|||
| RD-EG-VEGF-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 431 |
Mouse Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit |
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| RD-EG-VEGF-Mu-48Tests | Reddot Biotech | 48 Tests | EUR 429 |
Mouse Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit |
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| RD-EG-VEGF-Mu-96Tests | Reddot Biotech | 96 Tests | EUR 591 |
Rat Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit |
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| RD-EG-VEGF-Ra-48Tests | Reddot Biotech | 48 Tests | EUR 450 |
Rat Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit |
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| RD-EG-VEGF-Ra-96Tests | Reddot Biotech | 96 Tests | EUR 622 |
Bovine Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit |
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| RDR-EG-VEGF-b-48Tests | Reddot Biotech | 48 Tests | EUR 516 |
Bovine Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit |
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| RDR-EG-VEGF-b-96Tests | Reddot Biotech | 96 Tests | EUR 716 |
Human Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit |
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| RDR-EG-VEGF-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 330 |
Human Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit |
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| RDR-EG-VEGF-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 450 |
Mouse Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit |
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| RDR-EG-VEGF-Mu-48Tests | Reddot Biotech | 48 Tests | EUR 447 |
Mouse Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit |
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| RDR-EG-VEGF-Mu-96Tests | Reddot Biotech | 96 Tests | EUR 618 |
Rat Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit |
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| RDR-EG-VEGF-Ra-48Tests | Reddot Biotech | 48 Tests | EUR 470 |
Rat Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit |
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| RDR-EG-VEGF-Ra-96Tests | Reddot Biotech | 96 Tests | EUR 651 |
EG-VEGF |
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| PR29003 | Neuromics | 5 ug | EUR 231 |
EG-VEGF Antibody |
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| 3489-100 | Biovision | EUR 338 | |
EG-VEGF Antibody |
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| 3489R-100 | Biovision | EUR 316 | |
EG-VEGF Antibody |
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| 3489R-30T | Biovision | EUR 146 | |
EG VEGF antibody |
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| 20R-1607 | Fitzgerald | 100 ug | EUR 673 |
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Description: Rabbit polyclonal EG VEGF antibody |
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EG VEGF antibody |
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| 70R-EG002 | Fitzgerald | 50 ug | EUR 273 |
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Description: Affinity purified Goat polyclonal EG VEGF antibody |
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EG VEGF protein |
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| 30R-AE013 | Fitzgerald | 20 ug | EUR 273 |
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Description: Purified recombinant Human EG VEGF protein |
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rHu VEGF-165 |
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| AK8295-0002 | Akron Biotech | 2µg | Ask for price |
rHu VEGF-165 |
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| AK8295-0010 | Akron Biotech | 10µg | Ask for price |
rHu VEGF-165 |
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| AK8295-0100 | Akron Biotech | 100µg | Ask for price |
rHu VEGF-165 |
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| AK8295-1000 | Akron Biotech | 1mg | Ask for price |
rHu VEGF-121 |
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| AK8331-0002 | Akron Biotech | 2µg | Ask for price |
rHu VEGF-121 |
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| AK8331-0010 | Akron Biotech | 10µg | Ask for price |
rHu VEGF-121 |
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| AK8331-0100 | Akron Biotech | 100µg | Ask for price |
rHu VEGF-121 |
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| AK8331-1000 | Akron Biotech | 1mg | Ask for price |
rHu VEGF (cGMP) |
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| AK9063-0000 | Akron Biotech | 1mg | Ask for price |
EG-VEGF Blocking Peptide |
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| 20-abx064278 | Abbexa |
|
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Polyclonal EG-VEGF Antibody |
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| APG00002G | Leading Biology | 0.1ml | EUR 484 |
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Description: A polyclonal antibody raised in Goat that recognizes and binds to Human EG-VEGF . This antibody is tested and proven to work in the following applications: |
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Polyclonal EG-VEGF Antibody |
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| APR00114G | Leading Biology | 0.1mg | EUR 484 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human EG-VEGF . This antibody is tested and proven to work in the following applications: |
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EG-VEGF, human recombinant |
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| 4571-10 | Biovision | EUR 153 | |
EG-VEGF, human recombinant |
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| 4571-1000 | Biovision | EUR 2986 | |
EG-VEGF, human recombinant |
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| 4571-50 | Biovision | EUR 403 | |
EG-VEGF Recombinant Protein |
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| 40-629 | ProSci | 5 ug | EUR 259.25 |
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Description: EG-VEGF is a secreted angiogenetic mitogen growth factor expressed in the steroidogenic glands, ovary, testis, adrenal gland, and placenta. EG-VEGF induces proliferation, migration, and fenestration (formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. The human EG-VEGF gene codes for a 105 amino acid polypeptide containing an N-terminal signal sequence of 19 amino acids. Recombinant Human EG-VEGF is a 9.6 kDa protein consisting of 86 amino acid residues, including ten cysteine residues that potentially form five pairs of intra-molecular disulfide bonds. |
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EG-VEGF Recombinant Protein |
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| 40-702 | ProSci | 5 ug | EUR 259.25 |
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Description: EG-VEGF is a secreted angiogenetic mitogen growth factor expressed in the steroidogenic glands, ovary, testis, adrenal gland, and placenta. EG-VEGF induces proliferation, migration, and fenestration (formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. The murine EG-VEGF gene codes for a 105 amino acid polypeptide containing an N-terminal signal sequence of 19 amino acids. Recombinant Murine EG-VEGF is a 9.6 kDa protein consisting of 86 amino acid residues, including ten cysteine residues that potentially form five pairs of intra-molecular disulfide bonds. |
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Sheep EG-VEGF ELISA Kit |
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| ESE0137 | Abclonal | 96Tests | EUR 521 |
Mouse EG-VEGF ELISA Kit |
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| EME0137 | Abclonal | 96Tests | EUR 521 |
Monkey EG-VEGF ELISA Kit |
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| EMKE0137 | Abclonal | 96Tests | EUR 521 |
Porcine EG-VEGF ELISA Kit |
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| EPE0137 | Abclonal | 96Tests | EUR 521 |
Rat EG-VEGF ELISA Kit |
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| ERE0137 | Abclonal | 96Tests | EUR 521 |
Rabbit EG-VEGF ELISA Kit |
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| ERTE0137 | Abclonal | 96Tests | EUR 521 |
Anserini EG-VEGF ELISA Kit |
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| EAE0137 | Abclonal | 96Tests | EUR 521 |
Goat EG-VEGF ELISA Kit |
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| EGTE0137 | Abclonal | 96Tests | EUR 521 |
Canine EG-VEGF ELISA Kit |
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| ECE0137 | Abclonal | 96Tests | EUR 521 |
Chicken EG-VEGF ELISA Kit |
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| ECKE0137 | Abclonal | 96Tests | EUR 521 |
Human EG-VEGF ELISA Kit |
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| EHE0137 | Abclonal | 96Tests | EUR 521 |
Bovine EG-VEGF ELISA Kit |
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| EBE0137 | Abclonal | 96Tests | EUR 521 |
Prokineticin 1 (EG-VEGF) Antibody |
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| 20-abx007755 | Abbexa |
|
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EG-VEGF ELISA KIT|Human |
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| EF000094 | Lifescience Market | 96 Tests | EUR 689 |
Human EG-VEGF ELISA kit |
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| LF-EK50695 | Abfrontier | 1×96T | EUR 648 |
Human EG-VEGF PicoKine ELISA Kit |
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| EK0575 | BosterBio | 96 wells | EUR 456 |
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Description: For quantitative detection of human EG-VEGF in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
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Guinea Pig EG-VEGF ELISA Kit |
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| EGE0137 | Abclonal | 96Tests | EUR 521 |
EG-VEGF ELISA Kit (Human) (OKBB00283) |
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| OKBB00283 | Aviva Systems Biology | 96 Tests | EUR 544 |
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Description: Description of target: EG-VEGF, Endocrine glandderived vascular endothelial growth factor, is a human protein encoded by by the PROKR1 gene. Its gene is mapped to chromosome 1. The 1.4-kb cDNA encodes a protein of 105 amino acids with a well defined signal sequence. The mature protein is predicted to have 86 amino acids. Northern blot analysis demonstrated expression in testis, ovary, adrenal gland, and placenta. EG-VEGF has played a vital role in inducing proliferation, migration, and fenestration in capillary endothelial cells derived from endocrine glands.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: < 7 pg/mL |
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ELISA kit for Human EG-VEGF |
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| EK5247 | SAB | 96 tests | EUR 553 |
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Description: Enzyme-linked immunosorbent assay kit for quantification of Human EG-VEGF in samples from serum, plasma, tissue homogenates and other biological fluids. |
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Human EG-VEGF ELISA kit (4X96T) |
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| LF-EK50696 | Abfrontier | 4×96T | EUR 2201 |
Human Prokineticin 1 (EG-VEGF) CLIA Kit |
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| 20-abx190217 | Abbexa |
|
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Mouse Prokineticin 1 (EG-VEGF) CLIA Kit |
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| 20-abx190506 | Abbexa |
|
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Rat Prokineticin 1 (EG-VEGF) CLIA Kit |
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| 20-abx190672 | Abbexa |
|
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Cow Prokineticin 1 (EG-VEGF) CLIA Kit |
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| 20-abx190834 | Abbexa |
|
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Human prokineticin 1(PROK1/EG-VEGF) ELISA kit |
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| CSB-E04532h-24T | Cusabio | 1 plate of 24 wells | EUR 165 |
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Description: Quantitativesandwich ELISA kit for measuring Human prokineticin 1 (PROK1/EG-VEGF) in samples from serum. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price. |
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Human prokineticin 1(PROK1/EG-VEGF) ELISA kit |
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| 1-CSB-E04532h | Cusabio |
|
|
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Description: Quantitativesandwich ELISA kit for measuring Human prokineticin 1(PROK1/EG-VEGF) in samples from serum. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits. |
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EG-VEGF ELISA Kit (Human) : 96 Wells (OKAG00013) |
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| OKAG00013 | Aviva Systems Biology | 96 Wells | EUR 596 |
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Description: Description of target: The protein encoded by this gene induces proliferation, migration, and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. It has little or no effect on a variety of other endothelial and non-endothelial cell types. Its expression is restricted to the steroidogenic glands (ovary, testis, adrenal, and placenta), is induced by hypoxia, and often complementary to the expression of vascular endothelial growth factor (VEGF), suggesting that these molecules function in a coordinated manner.;Species reactivity: Human;Application: ELISA;Assay info: Quantitative Colorimentric Sandwich ELISA;Sensitivity: 16 pg/mL |
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Recombinant Mouse EG VEGF Protein, Untagged, E.coli-1mg |
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| QP11757-1mg | EnQuireBio | 1mg | EUR 2757 |
Recombinant Mouse EG VEGF Protein, Untagged, E.coli-20ug |
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| QP11757-20ug | EnQuireBio | 20ug | EUR 201 |
Recombinant Mouse EG VEGF Protein, Untagged, E.coli-5ug |
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| QP11757-5ug | EnQuireBio | 5ug | EUR 155 |
Rat Endocrine gland vascular endothelial growth factor, EG- VEGF |
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| ELA-E0024r | Lifescience Market | 96 Tests | EUR 886 |
Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) Antibody |
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| 20-abx128904 | Abbexa |
|
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Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) Antibody |
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| 20-abx172221 | Abbexa |
|
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Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) Antibody |
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| 20-abx172222 | Abbexa |
|
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Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) Antibody |
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| 20-abx172223 | Abbexa |
|
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Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) Antibody |
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| 20-abx176250 | Abbexa |
|
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Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) Antibody |
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| 20-abx176251 | Abbexa |
|
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Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) Antibody |
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| 20-abx176252 | Abbexa |
|
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Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) Antibody |
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| 20-abx176253 | Abbexa |
|
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Active Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) |
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| 4-APA024Hu01 | Cloud-Clone |
|
|
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Description: Recombinant Human Endocrine Gland Derived Vascular Endothelial Growth Factor expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells |
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Recombinant Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) |
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| 4-RPA024Hu01 | Cloud-Clone |
|
|
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Description: Recombinant Human Endocrine Gland Derived Vascular Endothelial Growth Factor expressed in: E.coli |
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Human Recombinant EG-VEGF Protein (84aa, E. Coli), biologically active |
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| VEGF36-R-100 | Alpha Diagnostics | 100 ug | EUR 1991 |
Human Recombinant EG-VEGF Protein (84aa, E. coli), biologically active |
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| VEGF36-R-20 | Alpha Diagnostics | 20 ug | EUR 773 |
Human Recombinant EG-VEGF Protein (84aa, E. coli), biologically active |
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| VEGF36-R-5 | Alpha Diagnostics | 5 ug | EUR 324 |
EG-VEGF ELISA Kit| Rat Endocrine Gland Vascular Endothelial Grow |
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| EF017699 | Lifescience Market | 96 Tests | EUR 689 |
Recombinant Human EG-VEGF/ prokineticin-1 Protein, Untagged, E.coli-100ug |
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| QP10402-ec-100ug | EnQuireBio | 100ug | EUR 988 |
Recombinant Human EG-VEGF/ prokineticin-1 Protein, Untagged, E.coli-20ug |
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| QP10402-ec-20ug | EnQuireBio | 20ug | EUR 290 |
Recombinant Human EG-VEGF/ prokineticin-1 Protein, Untagged, E.coli-250ug |
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| QP10402-ec-250ug | EnQuireBio | 250ug | EUR 1731 |